● Muaj ob txoj hauv kev xaiv los ntawm nyob ntawm qhov xav tau qib ntawm genome tiav.
● Draft Genome Option: Kev nyeem luv luv nrog Illumina NovaSeq PE150.
● Ua kom tiav 0 qhov sib txawv genome.
● Kev nyeem ntev ntev ntawm Nanopore PromethION 48 lossis PacBio Revio rau kev sib dhos genome.
● Kev nyeem luv luv ntawm Illumina NovaSeq rau genome validation thiab kho qhov yuam kev (Nanopore) los yog tsim kom muaj ib daim ntawv genome.
●Zero-Gap Genome Guaranteed: Qhov no yog vim kev koom ua ke ntawm Illumina sequencing nrog ntev nyeem sequencing (Nanopore los yog PacBio).
●Ua tiav Bioinformatics Workflow:Qhov no suav nrog kev sib dhos genome thiab kev twv ua ntej ntawm ntau lub ntsiab lus genomic, cov lus piav qhia txog cov noob caj noob ces, thiab kev pom genome xws li Circos zaj duab xis.
●Kev txawj ntse: Nrog ntau tshaj 20,000 microbial genomes sib sau ua ke, BMKGENE coj ntau tshaj kaum xyoo ntawm kev paub, pab pawg kws tshuaj ntsuam xyuas zoo, cov ntsiab lus nthuav dav, thiab kev txhawb nqa zoo tom qab muag.
●Kev them nyiaj yug tom qab muag:Peb qhov kev cog lus txuas ntxiv dhau qhov kev ua tiav nrog rau 3 lub hlis tom qab kev muag khoom. Nyob rau lub sijhawm no, peb muab cov phiaj xwm taug qab, kev pab daws teeb meem, thiab Q&A ntu los daws cov lus nug ntsig txog cov txiaj ntsig.
●Muaj ntau lub tswv yim Sequencing muaj:Rau cov hom phiaj tshawb fawb sib txawv thiab cov kev xav tau ntawm genome tiav.
Kev pabcuam | Sequence Strategy |
Draft Genome | Illumina PE 150 100x |
0 Gap Genome | Nanopor 100x + Illumina PE 150 100x Or Pacbio HiFi 30x + Illumina PE150 100x (yeem) |
Concentration (ng / μL) | Tag nrho cov nyiaj (µg) | Ntim (µL) | OB 260/280 | OB 260/230 | |
PacBio | ≥ 20 | ≥1.2 | ≥ 20 | 1.7-2.2 | ≥1.0 |
Nanopor | ≥40 | ≥ 2 | ≥ 20 | 1.7-2.2 | 1.0-3.0 Nws |
Illumina | ≥1 | ≥0.06 | ≥ 20 | - | - |
· Cov kab mob: ≥3.5x1010 hlwb
Xws li Kev Tshawb Fawb Hauv qab no:
● Sequencing Data Quality Control
● Genome Assembly
● Genome Component Analysis: Kev kwv yees ntawm CDS thiab ntau yam genomic
● Cov lus piav qhia ua haujlwm nrog ntau cov ntaub ntawv dav dav (GO, KEGG, thiab lwm yam) thiab cov ntaub ntawv siab heev (CARD, VFDB, thiab lwm yam)
● Genome Visualization
Peb muab cov genome nyob rau hauv ib qho yooj yim nkag tau fasta hom ntawv thiab cov ntaub ntawv genome annotation (gff).
Gene annotation – GO
Gene annotation - CAZY carbohydrates
Genome visualization - Circos zaj dab neeg
Tshawb nrhiav cov kev nce qib uas tau pab txhawb los ntawm BMKGene cov kev pabcuam kab mob genome los ntawm kev sau cov ntawv tshaj tawm.
Dai, W. et al. (2023) 'Discovery of Bacteroides uniformis F18-22 as a Safe and Novel Probiotic Bacterium for the Treatment of Ulcerative Colitis from the Healthy Human Colon',International Journal of Molecular Sciences, 24(19), p. 14669. doi: 10.3390/IJMS241914669/S1.
Kang, Q. et al. (2021) 'Multidrug-resistant Proteus mirabilis cais nqa blaOXA-1 thiab blaNDM-1 los ntawm cov tsiaj qus hauv Suav teb: nce kev noj qab haus huv rau pej xeem',Integrative Zoology, 16(6), pp. 798–809. doi: 10.1111/1749-4877.12510 ib.
Wang, TT et al. (2017) 'Complete genome sequence of endophyte Bacillus flexus KLBMP 4941 qhia nws txoj kev loj hlob ntawm cov nroj tsuag txhawb nqa mechanism thiab caj ces hauv paus rau ntsev kam rau siab',Phau ntawv Journal of Biotechnology, 260, pp. 38–41. doi: 10.1016/J.JBIOTEC.2017.09.001.
Wang, X. et al. (2021) 'Multiple-Replicon Resistance Plasmids of Klebsiella Mediate Extensive Dissemination of Antimicrobial Genes',Frontiers hauv Microbiology, 12, ib. 754931. doi: 10.3389/FMICB.2021.754931/BIBTEX.