16S/18S/ITS Amplicon Sequencing-NGS
Kev Pabcuam Nta
● Sequencing platform: Illumina NovaSeq.
● Amplification ntawm luv luv cheeb tsam ntawm 16S, 18S thiab ITS, ntawm lwm yam amplification lub hom phiaj.
● Kev xaiv yooj yim ntawm amplicon.
● yav dhau los qhov kev paub dhau los nrog ntau lub hom phiaj nthuav dav.
Kev Pabcuam Zoo
●Tsis muaj kev cais tawm:Kev txheeb xyuas sai ntawm microbial muaj pes tsawg leeg hauv cov qauv ib puag ncig.
●Kev daws teeb meem siab: Hauv cov khoom qis-ntau ntau hauv cov qauv ib puag ncig.
●Siv dav dav: Ntau hom kev tshawb fawb hauv zej zog microbial.
●Comprehensive Bioinformatic Analysis: Qhov tseeb QIIME2 pob (ntau qhov kev pom rau hauv microbial ecology) nrog ntau yam kev soj ntsuam ntawm cov ntaub ntawv, annotation, OTU / ASV.
●Kev txawj ntse: Nrog 150 txhiab amplicon sequencing tej yaam num ua txhua xyoo, BMKGENE coj ntau tshaj kaum xyoo ntawm kev paub, pab pawg neeg soj ntsuam zoo heev, cov ntsiab lus dav, thiab kev txhawb nqa tom qab muag zoo heev.
Service Specifications
| Lub tsev qiv ntawv | Sequence Strategy | Cov ntaub ntawv pom zoo |
| Amplicon | Illumina PE 250 | 50/100/300K tags (Nyeem Pairs) |
Kev Pabcuam
| Concentration (ng / μL) | Tag nrho cov nyiaj (ng) | Ntim (µL) |
| ≥1 | ≥200 | ≥ 20 |
● Av / sludge: 1-2g
● Cov ntsiab lus hauv plab-tsiaj: 0.5-2g
● Cov ntsiab lus hauv plab-kab: 0.1-0.25g
● Cog nto (enriched sediment): 0.1-0.5g
● Fermentation broth enriched sediment): 0.1-0.5g
● Faeces (cov tsiaj loj): 0.5-2g
● Faeces (nas): 3-5grains
● Pulmonary alveolar lavage kua: lim ntawv
● Vaginal swab: 5-6 swabs
● Daim tawv nqaij / qhov chaw mos swab / qaub ncaug / qhov ncauj mos cov ntaub so ntswg / pharyngeal swab / rectal swab: 2-3 swabs
● Cov kab mob saum npoo av: lim ntawv
● Waterbody/air/biofilm: lim ntawv
● Endophytes: 1-2g
● Hniav Plaque: 0.5-1g
Kev Pabcuam Ua Haujlwm Flow
Qauv xa tuaj
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
Xws li cov kev txheeb xyuas hauv qab no:
- Raw cov ntaub ntawv zoo tswj
- OTU pawg / De-noise (ASV)
- OTU annotation
- Alpha diversity tsom xam: ntau qhov ntsuas, suav nrog Shannon, Simpson thiab ACE.
- Kev txheeb xyuas kev sib txawv ntawm Beta
- Inter-pab pawg tsom xam
- Kev txheeb xyuas kev sib txheeb: nruab nrab ntawm ib puag ncig yam thiab OUT muaj pes tsawg leeg thiab ntau haiv neeg
- 16S kev ua haujlwm noob kwv yees
Histogram ntawm taxonomic faib
taxonomic abundance clustering tshav kub daim ntawv qhia
Alpha diversity tsom xam: rarefaction nkhaus
beta diversity analysis: NMDS
Kev tsom xam Intergroup: LEFSE biomarker nrhiav pom
Tshawb nrhiav cov kev nce qib uas tau pab los ntawm BMKGene cov kev pabcuam amplicon sequencing nrog Illumina los ntawm kev sau cov ntawv tshaj tawm.
Dong, C. et al. (2022) 'Assembly, Core Microbiota, and Function of the Rhizosphere Av and Bark Microbiota in Eucommia ulmoides', Frontiers in Microbiology, 13. doi: 10.3389/FMICB.2022.855317/FULL.
Li, Y. et al. (2023) 'Synthetic bacterial consortia transplantation for the treatment of Gardnerella vaginalis-induced bacterial vaginosis in nas', Microbiome, 11(1), pp. 1–14. doi: 10.1186/s40168-023-01497-y
Yang, J., Fu, Y. thiab Liu, H. (2022) 'Microbiomes ntawm huab cua plua plav sau thaum lub sij hawm hauv av kaw bioregenerative life support sim "Lunar Palace 365"', Environmental Microbiomes, 17(1), pp. 1–20. ib: 10.1186/S40793-022-00399-0/FIGURES/8.
Yin, S. et al. (2022) 'Feedstock-dependent abundance of functional genes related to nitrogen transformation controlled nitrogen loss in composting', Bioresource Technology, 361, p. 127678. doi: 10.1016/J.BIORTECH.2022.127678.
