10 x Genomics Viscium spatial transcriptome
Cov txheej txheem technical
Nta
● Kev daws teeb meem: 100 μm
● Haiv taws inch: 55 μm
● Cov naj npawb ntawm pob: 4992
Kev Tshawb Fawb: 6.5 x 6.5 hli
Txhua qhov chaw bar barcoded yog thauj khoom nrog cov ntawv tshaj tawm ntawm 4 ntu:
- Poly (DT) tus Tsov tus tw rau mrna priming thiab CdNA synthesis
- Cim cov cim qhia ntau dua (UMI) los kho qhov tsis ncaj ncees
- spatial barcode
- Kev kho kab ua ntu zus ntawm kev nyeem ib nrab 1 ua ntu zus
H & E qhov chaw ntawm ntu
Qhov Zoo
●Kev Pabcuam One-Nres: Muab txhua yam kev kawm paub thiab cov txheej txheem kev txawj ntse, suav nrog Cryo-socking, spatial barcoding, chaw npaj cov tsev qiv ntawv, ua ntu zus thiab bioinformics.
● Cov pab pawg muaj txuj ci siab heev: Nrog kev paub dhau 250 cov nqaij hom thiab 100+ hom suav nrog rau tib neeg, nas, tsiaj, ntses thiab nroj tsuag.
●Lub sijhawm hloov tshiab ntawm tag nrho txoj haujlwm: Nrog kev tswj xyuas tag nrho ntawm kev sim ua kev sim.
●Cov txheej txheem Bioinformformics:Pob suav nrog 29 kev tshuaj xyuas thiab 100+ cov nuj nqis siab.
●Customized cov ntaub ntawv tsom xam thiab kev pom: muaj rau kev tshawb fawb sib txawv.
●Kev xaiv sib koom tes kev txheeb xyuas nrog ib leeg ntawm Cell MRNA Exportensing
Cov lus qhia
| Qauv Yuav Tsum Tau | Lub tsev qiv ntawv | Seitencing Lub Tswv Yim | Cov ntaub ntawv pom zoo | Kev Tswj Xyuas Zoo |
| Os-embedded Cryo Cov Qauv (Kev ua siab ntau tshaj: kwv yees. 6x6x6 mm³) 2 blocks ib qho piv txwv | 10x Visium CDNA Lub Tsev Qiv Ntawv | ICATINA PE150 | 50K PE nyeem rau ib qho chaw (60gb) | Rin> 7 |
Yog xav paub ntxiv txog cov qauv kev npaj thiab kev pabcuam workflow, thov koj tham nrog aBMKGENE CART
Kev Pabcuam Workflow
Hauv cov qauv npaj theem, ib qho pib RNA extraction sim yog ua kom pom zoo RNA tuaj yeem tau txais. Nyob rau hauv daim ntaub ua kom nce qib ntu yog stained thiab pom thiab permeagilization tej xwm txheej rau mrna tso tawm ntawm cov nqaij yog qhov zoo. Tom qab cov qauv zoo li qub tau thov thaum lub tsev qiv ntawv siv, ua raws kev ua ntu zus thiab cov ntaub ntawv tsom xam.
Qhov kev pabcuam ua tiav ua haujlwm koom nrog lub sijhawm tshiab thiab cov neeg siv tau lees paub los tswj cov vaj lug kub ua kom tiav, ua kom tiav txoj haujlwm du.
Xws li kev tsom xam hauv qab no:
Cov ntaub ntawv zoo tswj:
o Cov ntaub ntawv tawm thiab cov qhab nia zoo
o noob nrhiav kom pom kev
O nqaij npog
Cov kev txheeb xyuas sab hauv:
o gene kev nplua nuj
o Saib clustering, suav nrog txo qhov ntsuas kom tsawg
o Kev txheeb xyuas kev sib txawv ntawm cov pawg: kev txheeb xyuas cov cim cim
o Cov ntawv tshaj tawm ua haujlwm thiab cov khoom siv hluav taws xob ntxiv
Kev Ntsuam Xyuas Kev Tshawb Fawb
o Rov ua ke ntawm cov pob me me los ntawm ob qho hnoos qeev (piv txwv li muaj mob thiab tswj) thiab rov ua dua
o Kev txheeb xyuas cov cim cim rau txhua pawg
o Cov ntawv tshaj tawm ua haujlwm thiab cov khoom siv hluav taws xob ntxiv
o Cov kev qhia sib txawv ntawm tib pawg ntawm pawg
Sab hauv-qauv tshuaj ntsuam
Cov nqaij sawv
Cim Cov Lus Cim Cov Lus Qhia thiab Kev faib tawm spatial
Kev Ntsuam Xyuas Kev Tshawb Fawb
Cov ntaub ntawv sib xyaw los ntawm ob pawg thiab rov ua dua
Cim Cov Av ntawm Cov Txheej Txheem Tshiab
Tshawb nrhiav cov kev nce qib uas yooj yim los ntawm BMKGAE lub spatial transcriptomics los ntawm 10 x Visium hauv cov ntaub ntawv tshaj tawm no:
Chen, D. li al. (2023) 'Mthl1, lub peev xwm Drosophila homologue Gampalian adhesion GPCRS, yog koom nrog cov tshuaj tiv thaiv kab mob rau txhaj tshuaj rau hauv yoov',Kev hais plaub ntawm National Academy ntawm kev tshawb fawb ntawm Tebchaws Meskas, 120 (30), p. E2303462120. Doi: /10.1073/pnas.23034626220
Chen, Y. li al. (2023) 'Cov hlau ua kom cov kev daws teeb meem siab ntawm cov ntaub ntawv spatioLevoral',Cov lus qhia hauv Bioinform, 24 (2), pp. 1-10. DOI: 10.1093 / Bib / BBAD068.
Liu, C. li al. (2022) 'lub spatioCerempendentalal atlas ntawm Organogenesis hauv kev txhim kho ntawm Orchid Paj',Kev tshawb fawb nucleic acids, 50 (17), pp. 9724-9737. DOI: 10.1093 / nar / gkac773.
Wang, J. li al. (2023) '' '' '' Nucleus RNA excortomics qhia txog cov tswv yim kho tau rau uterine leiomyoma ',Ntiaj Teb Xov Xwm ntawm Biological Sciences, 19 (8), pp. 2515-2530. DOI: 10.7150 / iJbs.83510.
