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Nā hale waihona puke i hana mua ʻia e DNSSEQ
ʻO DNBSEQ, i hoʻomohala ʻia e MGI, he ʻenehana NGS hou i hoʻokō i ka hoʻohaʻahaʻa ʻana i nā kumukūʻai sequencing a hoʻonui i ka throughput. ʻO ka hoʻomākaukau ʻana i nā hale waihona puke DNBSEQ e pili ana i ka ʻāpana DNA, ka hoʻomākaukau ʻana i ka ssDNA, a me ka hoʻonui ʻia ʻana o ka pōʻai no ka loaʻa ʻana o nā DNA nanoballs (DNB). Hoʻouka ʻia kēia mau mea ma luna o kahi ʻili paʻa a hoʻopaʻa ʻia e ka hui ʻana o Probe-Anchor Synthesis (cPAS). Hoʻohui ka ʻenehana DNBSEQ i nā pōmaikaʻi o ka loaʻa ʻana o ka hapa haʻahaʻa haʻahaʻa haʻahaʻa me ka hoʻohana ʻana i nā hiʻohiʻona hewa kiʻekiʻe me nā nanoballs, ka hopena i ke kaʻina me ka throughput kiʻekiʻe a me ka pololei.
ʻO kā mākou lawelawe hoʻonohonoho waihona puke i hana mua ʻia e hiki ai i nā mea kūʻai ke hoʻomākaukau i nā hale waihona puke Illumina sequencing mai nā kumu like ʻole (mRNA, genome holoʻokoʻa, amplicon, 10x libraries, a me nā mea ʻē aʻe), i hoʻohuli ʻia i nā hale waihona MGI i kā mākou laboratories e hoʻonohonoho ʻia ma DNBSEQ-T7, e hiki ai. nā huina ʻikepili kiʻekiʻe ma nā kumukūʻai haʻahaʻa.
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Nā hale waihona puke i hana mua ʻia e Illumina
ʻO ka ʻenehana Sequencing Illumina, i hoʻokumu ʻia ma Sequencing by Synthesis (SBS), he hana hou NGS i kākoʻo ʻia i ka honua, kuleana no ka hoʻomohala ʻana ma luna o 90% o ka ʻikepili sequencing o ka honua. ʻO ke kumumanaʻo o SBS e pili ana i ke kiʻi ʻana i ka fluorescently labeled reversible terminator i ka hoʻohui ʻia ʻana o kēlā me kēia dNTP, a ma hope i hoʻokaʻawale ʻia e ʻae i ka hoʻohui ʻana i ka waihona aʻe. Me nā dNTP i hoʻopaʻa ʻia e hoʻololi hou ʻia e loaʻa i kēlā me kēia pōʻaiapili kaʻina, hoʻemi ka hoʻokūkū kūlohelohe i ka manaʻo hoʻohui. Kākoʻo kēia ʻenehana maʻalahi i nā hale waihona puke heluhelu hoʻokahi a i hoʻopaʻa ʻia, e mālama ana i nā ʻano hana genomic. Hiki i ka Illumina sequencing ke kiʻekiʻe a me ka pololei ke hoʻonoho iā ia ma ke ʻano he pōhaku kihi i ka noiʻi genomics, e hāʻawi i ka mana i nā ʻepekema e wehe i nā mea paʻakikī o nā genomes me nā kikoʻī like ʻole a me ka maikaʻi.
ʻO kā mākou lawelawe hoʻonohonoho waihona puke i hana mua ʻia e hiki ai i nā mea kūʻai ke hoʻomākaukau i nā hale waihona puke mai nā kumu like ʻole (mRNA, genome holoʻokoʻa, amplicon, 10x libraries, a me nā mea ʻē aʻe). A laila, hiki ke hoʻouna ʻia kēia mau hale waihona puke i kā mākou mau kikowaena sequencing no ka hoʻokele maikaʻi a me ka hoʻonohonoho ʻana i nā kahua ʻo Illumina.
