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Mea paahana
ʻO BMKCloud kahi papahana bioinformatic alakaʻi e hāʻawi ana i ka hopena hoʻokahi no nā papahana genomic, kahi i hilinaʻi nui ʻia e nā mea noiʻi ma nā ʻano ʻano like ʻole me ka lāʻau lapaʻau, ka mahiʻai, ka nohona, a me nā mea ʻē aʻe. , nā kumuwaiwai helu helu, ʻikepili lehulehu, bioinformtic online papa, etc. BMKCloud loaʻa i nā mea hana bioinformatic i hoʻohana pinepine ʻia e pili ana i ka gene annotation, evolutionary. nā mea hana genetic, ncRNA, ka mālama ʻana i ka maikaʻi o ka ʻikepili, ka hui ʻana, ka hoʻonohonoho ʻana, ka ʻohi ʻana i ka ʻikepili, nā hoʻololi ʻana, nā helu helu, ka mea hoʻomohala kiʻi, ka nānā ʻana i ke kaʻina, etc.
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ʻO De novo Bacterial Genome Assembly
Hāʻawi mākou i kahi lawelawe hui genome bacterial piha, e hōʻoiaʻiʻo ana i 0 mau āpau. Hiki kēia ma ka hoʻohui ʻana i nā ʻenehana sequencing heluhelu lōʻihi, e like me Nanopore a me PacBio no ka ʻākoakoa a me ka heluhelu pōkole ʻana me Illumina no ka hōʻoia ʻana o ka hui a me ka hoʻoponopono hewa ʻana o nā heluhelu ONT. Hāʻawi kā mākou lawelawe i ka holo holoʻokoʻa bioinformatic piha mai ka hui ʻana, ka annotation hana, a me ka loiloi bioinformatic holomua, e hoʻokō ana i nā pahuhopu noiʻi kikoʻī. Hāʻawi kēia lawelawe i ka hoʻomohala ʻana i nā genomes kuhikuhi pololei no nā haʻawina genetic a me genomic. Hoʻohui ʻia, hana ia i kumu no nā noi e like me ka strain optimization, genetic engineering, a me ka hoʻomohala ʻenehana microbial, e hōʻoiaʻiʻo ana i ka ʻikepili genomic hilinaʻi a me ka gap-free koʻikoʻi no ka holomua ʻana i ka ʻike ʻepekema a me ka hana hou biotechnological.
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RNA liʻiliʻi
ʻO nā RNA liʻiliʻi he ʻano pōkole non-coding RNA me ka lōʻihi o 18-30 nt, me ka miRNA, siRNA a me ka piRNA. Ua hōʻike nui ʻia kēia mau RNA liʻiliʻi e pili ana i nā kaʻina hana ola like ʻole e like me ka hoʻohaʻahaʻa ʻana o ka mRNA, ka pāpā ʻana i ka unuhi, ka hoʻokumu ʻana o heterochromatin, a me nā mea ʻē aʻe. ʻO ka papa hana hoʻopaʻa ʻana kaʻina hana maʻamau a me ka ʻimi ʻikepili kiʻekiʻe. Ma ke kumu o ka ʻikepili RNA-seq, hiki i ka loiloi maʻamau ke hoʻokō i ka ʻike miRNA a me ka wānana, ka wānana gene target miRNA, annotation a me ka nānā ʻana i ka ʻōlelo. Hiki i ka loiloi holomua ka huli ʻana a me ka unuhi ʻana o ka miRNA, ka hana kiʻi Venn, ka miRNA a me ka kūkulu ʻana i ka ʻoihana gene.
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NGS-WGS (Illumina/BGI)
ʻO NGS-WGS kahi kahua hōʻano hou genome re-sequencing ana, i hoʻomohala ʻia ma ke kumu o ka ʻike waiwai ma Biomarker Technologies. Hāʻawi kēia paepae maʻalahi i ka hoʻouna wikiwiki ʻana i kahi kaila hana hoʻopili i hoʻohui ʻia ma o ka hoʻonohonoho ʻana i kekahi mau palena maʻamau, i kūpono i ka ʻikepili sequencing DNA i hana ʻia mai ka paepae Illumina a me BGI sequencing platform. Hoʻokomo ʻia kēia kahua ma luna o ka server computing hana kiʻekiʻe, e hāʻawi ana i ka mana o ka nānā ʻana i ka ʻikepili kūpono loa i ka manawa pōkole loa. Loaʻa ka mining data pilikino ma ke kumu o ka nānā ʻana maʻamau, me ka huli ʻana i nā gene mutated, PCR primer design, etc.
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mRNA(Hōʻike)
ʻO Transcriptome ka loulou ma waena o ka ʻike genetic genomic a me ka proteome o ka hana biological. ʻO ka hoʻoponopono pae transcription ka mea koʻikoʻi a ʻo ke ʻano hoʻoponopono i aʻo nui ʻia o nā meaola. Hiki i ka transcriptome sequencing ke hoʻonohonoho i ka transcriptome i kēlā me kēia manawa i ka manawa a i ʻole ma lalo o kekahi kūlana, me ka hoʻoholo pololei i ka nucleotide hoʻokahi. laʻana i nā palapala kikoʻī.
I kēia manawa, ua hoʻohana nui ʻia ka ʻenehana sequencing transcriptome i ka agronomy, ka lāʻau lapaʻau a me nā ʻano noiʻi ʻē aʻe, me ka hoʻoponopono ʻana i nā holoholona a me nā mea kanu, ka hoʻoponopono ʻana i ke kaiapuni, ka launa pū ʻana, ka localization gene, species genetic evolution and tumor and genetic disease detection.
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Metagenomics (NGS)
Hoʻolālā ʻia kēia kahua hoʻopaʻa inoa no ka ʻikepili ʻikepili metagenomic shotgun ma ke kumu o nā makahiki o ka ʻike. Loaʻa iā ia ke kahe hana i hoʻohui ʻia i loko o nā ʻano loiloi metagenomics maʻamau e pono ai e pili ana i ka hoʻoili ʻana i ka ʻikepili, nā haʻawina pae ʻano, nā haʻawina pae hana gene, metagenome binning, a me nā mea ʻē aʻe. , hoʻonohonoho hoʻonohonoho ʻāpana, hana kiʻi pilikino, etc.
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LncRNA
ʻO nā RNA non-coding lōʻihi (lncRNA) kekahi ʻano o nā transcript me ka lōʻihi ma mua o 200 nt, ʻaʻole hiki ke hoʻopaʻa i nā protein. Hōʻike nā hōʻike hōʻuluʻulu e hiki ke hana ka hapa nui o nā lncRNA. Hiki iā mākou ke hōʻike i nā kaʻina lncRNA a me ka hoʻonohonoho ʻana i ka ʻike a alakaʻi iā mākou e ʻike i nā lncRNA me nā hana hoʻoponopono koʻikoʻi. Haʻaheo ʻo BMKCloud e hāʻawi i kā mākou mea kūʻai aku lncRNA sequencing analysis platform e hoʻokō i ka loiloi lncRNA wikiwiki, hilinaʻi a maʻalahi.
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GWAS
ʻO ka Genome-wide association study (GWAS) ka manaʻo e ʻike i nā ʻano ʻano genetic (genotype) e pili ana i nā ʻano kikoʻī (phenotype). Ke noiʻi nei nā haʻawina GWA i nā hōʻailona genetic e hele i ka genome holoʻokoʻa o ka heluna nui o nā kānaka a wānana i nā hui genotype-phenotype ma o ka loiloi helu helu ma ka pae heluna kanaka. Hiki ke ʻike i nā ʻano genetic āpau i ka resequencing holoʻokoʻa. I ka hui pū ʻana me ka ʻikepili phenotypic, hiki ke hana ʻia ʻo GWAS e ʻike i nā SNP pili phenotype, QTL a me nā genes moho, e kākoʻo ikaika ana i ka hoʻohua holoholona/mea kanu o kēia wā. ʻO SLAF kahi hoʻolālā hoʻolālā genome maʻalahi i hoʻomohala ʻia, kahi e ʻike ai i nā māka hoʻolaha genome-ākea, SNP. ʻO kēia mau SNP, ma ke ʻano he molecular genetic markers, hiki ke hana ʻia no nā haʻawina hui me nā ʻano i kuhikuhi ʻia. He hoʻolālā kumu kūʻai i ka ʻike ʻana i nā ʻano paʻakikī e pili ana i nā ʻano like ʻole.
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Nanopore Ka lōʻihi lōʻihi
ʻO nā isoforms paʻakikī a me nā ʻano like ʻole i loko o nā mea ola he mau ʻano genetic koʻikoʻi no ka hoʻoponopono ʻana i ka hōʻike gene a me ka ʻokoʻa protein. ʻO ka ʻike pololei ʻana i nā hale transcript ke kumu no ka noiʻi hohonu ʻana i nā ʻano hoʻoponopono hoʻoponopono hōʻike gene. Ua hoʻokō maikaʻi ʻo Nanopore sequencing platform i ka haʻawina transcriptomic i ka pae isoform. Hoʻolālā ʻia kēia kahua hoʻopaʻa ʻana e kālailai i ka ʻikepili RNA-Seq i hana ʻia ma ke kahua Nanopore ma ke kumu o ka genome reference, kahi e loaʻa ai nā loiloi qualitative a me quantitative ma nā pae gene a me nā pae transcripts.
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pōʻai-RNA
ʻO Circular RNA(circRNA) kahi ʻano o ka RNA non-coding, i ʻike ʻia i kēia manawa e pāʻani i ke kuleana koʻikoʻi i nā ʻoihana hoʻoponopono e pili ana i ka hoʻomohala ʻana, ke kū ʻana o ke kaiapuni, a me nā mea ʻē aʻe. Hoʻohui ʻia nā ʻaoʻao o ka circRNA e hana i kahi ʻano pōʻai, e hoʻopakele iā lākou mai ka ʻeli ʻana o exonuclease a ʻoi aku ka paʻa ma mua o ka hapa nui o ka RNA linear. Ua ʻike ʻia ʻo CircRNA he mau hana like ʻole i ka hoʻoponopono ʻana i ka hōʻike gene. Hiki i ka CircRNA ke hana ma ke ʻano he ceRNA, nāna e hoʻopaʻa i ka miRNA me ka hoʻokūkū, i kapa ʻia ʻo miRNA sponge. Hāʻawi ka CircRNA sequencing analysis platform i ka hoʻolālā circRNA a me ka nānā ʻana i ka hōʻike, ka wānana pahuhopu a me ka loiloi hui pū me nā ʻano mole RNA ʻē aʻe.
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BSA
Loaʻa i ka paena Bulked Segregant Analysis kahi ʻano hoʻohālikelike maʻamau hoʻokahi a me ka nānā ʻana kiʻekiʻe me ka hoʻonohonoho hoʻonohonoho maʻamau. ʻO ka BSA kahi ʻenehana i hoʻohana ʻia e ʻike koke i nā hōʻailona genetic pili phenotype. Aia i loko o ke kaʻina hana nui o BSA: 1. ke koho ʻana i ʻelua pūʻulu kanaka me nā phenotypes kūʻē loa; 2. ka hoʻohui ʻana i ka DNA, RNA a i ʻole SLAF-seq (Hoʻokumu ʻia e Biomarker) o kēlā me kēia kanaka e hana i ʻelua nui o ka DNA; 3. ka ʻike ʻana i nā kaʻina like ʻole e pili ana i ka genome kuhikuhi a i ʻole ma waena, 4. wānana i nā wahi i hoʻopili ʻia e ka moho e ED a me SNP-index algorithm; 5. Ka nānā 'ana a me ka ho'onui 'ana i nā genes ma nā wahi moho, a me nā mea 'ē a'e. Loa'a nō ho'i ka 'eli 'ana i ka 'ikepili me ka nānā 'ana i ka genetic marker a me ka ho'olālā kumu.
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Amplicon (16S/18S/ITS)
Hoʻokumu ʻia ka paepae Amplicon (16S/18S/ITS) me nā makahiki o ka ʻike ʻana i ka noiʻi papahana microbial diversity, kahi i hoʻopaʻa ʻia i ka nānā ʻana maʻamau a me ka loiloi pilikino: uhi ʻia ka loiloi kumu i ka ʻike loiloi kumu o ka noiʻi microbial o kēia manawa, waiwai a piha ka ʻikepili. a hōʻike ʻia nā hopena o ka nānā ʻana ma ke ʻano o nā hōʻike papahana; He ʻokoʻa ka ʻike o ka loiloi pilikino. Hiki ke koho ʻia nā laʻana a hiki ke hoʻonohonoho pono ʻia nā ʻāpana e like me ka hōʻike loiloi kumu a me ke kumu noiʻi, e ʻike ai i nā koi pilikino. ʻO ka ʻōnaehana hana Windows, maʻalahi a wikiwiki.
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Nā Genetics Evolutionary
ʻO ka Evolutionary Genetics kahi lawelawe hoʻonohonoho holoʻokoʻa i hoʻolālā ʻia e hāʻawi i kahi wehewehe ʻike o ka evolution i loko o kahi pūʻulu nui o nā kānaka, e pili ana i nā ʻano like ʻole, me nā SNP, InDels, SVs, a me CNVs. Hoʻopili kēia lawelawe i nā loiloi koʻikoʻi a pau e pono ai e wehewehe i nā loli evolutionary a me nā hiʻohiʻona genetic o ka heluna kanaka, me ka loiloi o ka hoʻonohonoho ʻana o ka heluna kanaka, ka ʻokoʻa genetic, a me nā pilina phylogenetic. Eia kekahi, ʻimi ʻo ia i nā haʻawina e pili ana i ke kahe ʻana o ke ʻano, e hiki ai i nā manaʻo o ka nui o ka heluna kanaka kūpono a me ka manawa ʻokoʻa. Hāʻawi nā haʻawina genetics Evolutionary i nā ʻike waiwai i ke kumu a me ka hoʻololi ʻana o nā ʻano.
Ma BMKGENE, hāʻawi mākou i ʻelua ala no ka hoʻokō ʻana i nā haʻawina genetics evolutionary ma nā heluna nui: hoʻohana i ka holoʻokoʻa-genome sequencing (WGS) a i ʻole ke koho ʻana i kahi ʻano hoʻohālikelike genome sequencing hōʻemi, ka Specific-Locus Amplified Fragment (SLAF) i kūkulu ʻia i loko o ka hale. ʻOiai ua kūpono ka WGS i nā genome liʻiliʻi, ua puka mai ʻo SLAF ma ke ʻano he kumu kūʻai kūpono no ke aʻo ʻana i nā heluna nui me nā genome lōʻihi, e hōʻemi pono ana i nā kumukūʻai sequencing.
