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BMKMANU S3000_Spatial Transcriptome
Kū ka Spatial transcriptomics ma ke alo o ka ʻepekema ʻepekema, e hāʻawi ana i ka poʻe noiʻi e ʻimi i nā hiʻohiʻona hōʻike gene paʻakikī i loko o nā ʻiʻo me ka mālama ʻana i kā lākou pōʻaiapuni spatial. Ma waena o nā paepae like ʻole, ua hoʻomohala ʻo BMKGene i ka BMKManu S3000 Spatial Transcriptome Chip, e kaena ana i kahi hoʻonā i hoʻonui ʻia o 3.5µm, hiki i ka laulā subcellular, a hiki i nā hoʻonohonoho hoʻonā pae-nui. ʻO ka puʻupuʻu S3000, e hōʻike ana ma kahi o 4 miliona mau wahi, hoʻohana i nā microwells i hoʻopaʻa ʻia me nā pahu i hoʻopiha ʻia me nā probes hopu hopu spatially barcoded. Ua hoʻomākaukau ʻia kahi waihona cDNA, i hoʻonui ʻia me nā barcodes spatial, mai ka chip S3000 a ma hope i hoʻonohonoho ʻia ma ke kahua ʻo Illumina NovaSeq. ʻO ka hui pū ʻana o nā hōʻailona spatially barcoded a me nā UMI e hōʻoia i ka pololei a me ka kikoʻī o ka ʻikepili i hana ʻia. ʻO ka puʻupuʻu BMKManu S3000 he mea maʻalahi loa, e hāʻawi ana i nā hoʻonohonoho hoʻonā i nā pae he nui i hiki ke hoʻoponopono maikaʻi ʻia i nā ʻiʻo like ʻole a me nā pae kikoʻī i makemake ʻia. Hoʻonohonoho kēia adaptability i ka chip ma ke ʻano he koho maikaʻi loa no nā haʻawina transcriptomics spatial like ʻole, e hōʻoia ana i ka clustering spatial pololei me ka leo liʻiliʻi. ʻO ka hoʻohana ʻana i ka ʻenehana hoʻokaʻawale kelepona me BMKManu S3000 hiki ke hoʻokaʻawale i ka ʻikepili transcriptional i nā palena o nā cell, ka hopena i kahi loiloi i loaʻa ka manaʻo biological pololei. Eia kekahi, ʻoi aku ka maikaʻi o ka hoʻonā ʻana o S3000 i ka nui o nā genes a me nā UMI i ʻike ʻia i kēlā me kēia cell, e hiki ai i ka nānā pono ʻana i nā ʻano kikoʻī spatial a me ka hui ʻana o nā cell.
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Nā hale waihona puke i hana mua ʻia e DNSSEQ
ʻO DNBSEQ, i hoʻomohala ʻia e MGI, he ʻenehana NGS hou i hoʻokō i ka hoʻohaʻahaʻa ʻana i nā kumukūʻai sequencing a hoʻonui i ka throughput. ʻO ka hoʻomākaukau ʻana i nā hale waihona puke DNBSEQ e pili ana i ka ʻāpana DNA, ka hoʻomākaukau ʻana i ka ssDNA, a me ka hoʻonui ʻia ʻana o ka pōʻai no ka loaʻa ʻana o nā DNA nanoballs (DNB). Hoʻouka ʻia kēia mau mea ma luna o kahi ʻili paʻa a hoʻopaʻa ʻia e ka hui ʻana o Probe-Anchor Synthesis (cPAS). Hoʻohui ka ʻenehana DNBSEQ i nā pōmaikaʻi o ka loaʻa ʻana o ka hapa haʻahaʻa haʻahaʻa haʻahaʻa me ka hoʻohana ʻana i nā hiʻohiʻona hewa kiʻekiʻe me nā nanoballs, ka hopena i ke kaʻina me ka throughput kiʻekiʻe a me ka pololei.
ʻO kā mākou lawelawe hoʻonohonoho waihona puke i hana mua ʻia e hiki ai i nā mea kūʻai ke hoʻomākaukau i nā hale waihona puke Illumina sequencing mai nā kumu like ʻole (mRNA, genome holoʻokoʻa, amplicon, 10x libraries, a me nā mea ʻē aʻe), i hoʻohuli ʻia i nā hale waihona MGI i kā mākou laboratories e hoʻonohonoho ʻia ma DNBSEQ-T7, e hiki ai. nā huina ʻikepili kiʻekiʻe ma nā kumukūʻai haʻahaʻa.
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Hoʻopili Chromatin ma muli o Hi-C
ʻO Hi-C kahi ala i hoʻolālā ʻia e hopu i ka hoʻonohonoho genomic ma o ka hoʻohui ʻana i nā pilina pili pili kokoke a me ke kaʻina hana kiʻekiʻe. Hoʻokumu ʻia ke ʻano ma luna o ka chromatin crosslinking me formaldehyde, a ukali ʻia e ka ʻai ʻana a me ka re-ligation ma ke ʻano o nā ʻāpana i hoʻopili covalently e hana i nā huahana ligation. Ma ka hoʻonohonoho ʻana i kēia mau huahana ligation, hiki ke aʻo i ka hui 3D o ka genome. Hiki iā Hi-C ke aʻo i ka māhele ʻana o nā ʻāpana o ka genome i hoʻopili māmā ʻia (A compartments, euchromatin) a ʻoi aku ka ikaika o ka transcriptionally, a me nā wahi i paʻa paʻa loa (B compartments, Heterochromatin). Hiki ke hoʻohana ʻia ʻo Hi-C no ke kuhikuhi ʻana i nā Topologically Associated Domains (TADs), nā ʻāpana o ka genome i hoʻopili ʻia nā hale a loaʻa paha nā ʻano hōʻike like, a e ʻike i nā puka lou chromatin, nā ʻāpana DNA i hoʻopaʻa ʻia e nā protein a ʻo ia hoʻi. hoʻonui pinepine ʻia i nā mea hoʻoponopono. Hāʻawi ka lawelawe ʻo Hi-C sequencing ʻo BMKGene i ka poʻe noiʻi e ʻimi i nā ʻāpana spatial o ka genomics, e wehe ana i nā ala hou no ka hoʻomaopopo ʻana i ka hoʻoponopono genome a me kona hopena i ke olakino a me nā maʻi.
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TGuide Smart Magnetic Plant RNA Kit
TGuide Smart Magnetic Plant RNA Kit
E hoʻomaʻemaʻe i ka RNA piha kiʻekiʻe mai nā ʻiʻo mea kanu
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TGuide Smart Blood/Cell/Tissue RNA Kit
TGuide Smart Blood/Cell/Tissue RNA Kit
ʻO ka pahu pahu pahu / paʻi reagent i hoʻopiha mua ʻia no ka hoʻomaʻemaʻe ʻana i nā hua kiʻekiʻe, kiʻekiʻe-maʻemaʻe, kiʻekiʻe, kiʻekiʻe, inhibitor-free total RNA mai ka ʻiʻo holoholona / cell / koko holoʻokoʻa hou.
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TGuide Smart Magnetic Plant DNA Kit
TGuide Smart Magnetic Plant DNA Kit
E hoʻomaʻemaʻe i ka DNA genomic kiʻekiʻe mai nā ʻiʻo mea kanu like ʻole
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TGuide Smart Soil / Stool DNA Kit
TGuide Smart Soil / Stool DNA Kit
Hoʻomaʻemaʻe i ka DNA inhibitor-ʻole o ka maʻemaʻe kiʻekiʻe a me ka maikaʻi mai ka lepo a me nā mea hoʻohālike
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ʻO TGuide Smart DNA Purification Kit
Hoʻihoʻi hou i ka DNA kiʻekiʻe mai ka huahana PCR a i ʻole nā gel agarose.
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TGuide Smart Blood Genomic DNA Kit
TGuide Smart Blood Genomic DNA Kit
ʻO ka pahu pahu i hoʻopiha mua ʻia / pahu reagent kit no ka hoʻomaʻemaʻe DNA genomic mai ke koko a me ke kapa buffy
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TGuide Smart Magnetic Tissue DNA Kit
ʻO ka pahu pahu hoʻopiha piha / pahu reagent no ka lawe ʻana i ka DNA genomic mai nā ʻiʻo holoholona
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TGuide Smart Universal DNA Kit
ʻO ka pahu pahu pahu / paʻi reagent kit no ka hoʻomaʻemaʻe genomic DNA mai ke koko, kahi koko maloʻo, bacteria, cell, saliva, swabs waha, ʻiʻo holoholona, etc.
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TGuide S16 Nucleic Acid Extractor
TGuide S16 Nucleic Acid Extractor
Mea hoʻohana maʻalahi Benchtop, 1-8 a i ʻole 16 mau laʻana i ka manawa like
Helu helu / puʻupuʻu
Cat. ʻAʻole
ID
Ka helu o nā preps
OSE-S16-AM
1 hoʻonohonoho
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PacBio 2+3 Hoʻonā mRNA piha piha
ʻOiai ʻo NGS-based mRNA sequencing he mea hana maʻalahi no ka helu ʻana i ka hōʻike gene, ʻo kona hilinaʻi ʻana i nā heluhelu pōkole e kaupalena i kona pono i nā loiloi transcriptomic paʻakikī. Ma ka ʻaoʻao ʻē aʻe, hoʻohana ʻo PacBio sequencing (Iso-Seq) i ka ʻenehana heluhelu lōʻihi, e hiki ai i ke kaʻina o nā transcripts mRNA holoʻokoʻa. Hoʻomaʻamaʻa kēia ala i ka ʻimi holoʻokoʻa o ka splicing ʻē aʻe, ka hui pū ʻana, a me ka poly-adenylation, ʻoiai ʻaʻole ia ke koho mua no ka helu ʻana i nā ʻano gene. Hoʻopili ka hui 2+3 i ka ʻokoʻa ma waena o Illumina a me PacBio ma ka hilinaʻi ʻana i ka heluhelu ʻana o PacBio HiFi e ʻike i ka pūʻulu holoʻokoʻa o nā isoforms transcript a me NGS sequencing e helu i nā isoforms like.
Nā Papahana: PacBio Sequel II/ PacBio Revio a me Illumina NovaSeq;
