Pacbio Ka lōʻihi lōʻihi

ʻO ka PacBio Full-Length Transcriptome Sequencing, Isoseq, hiki ke ʻike pololei i nā isoforms transcript, e hoʻomālamalama i ka polyadenylation a me ka splicing ʻē aʻe, a ke alakaʻi nei i ka hōʻike hōʻikeʻike gene pololei. Hoʻolālā ʻia ka BMKCloud PacBio Full-Length Transcriptome pipeline e kālailai i nā waihona cDNA i hoʻonohonoho ʻia ma ke ʻano Circular Consensus Sequencing (CCS) a ʻike i nā kaʻina Full-Length Non-Chimeric (FLNC), a laila hui ʻia i loko o nā transcripts non-redundant. Hoʻopili ka loiloi BUSCO i ka piha o ka hui transcriptome. Mai ka transcriptome i hōʻuluʻulu ʻia, hana ʻia nā loiloi he nui: ʻokoʻa splicing, simple sequence repeat (SSR), wānana o lncRNA a me nā genes i hoʻopaʻa ʻia, wānana i nā genes moʻokalaleo, kānana ʻohana ʻohana, transcription factor analysis, a me ka helu hana o nā transcripts.