T2T GENOME ASSEMBLY, GAP FREE GENOME
1stʻElua Raiki Genomes1
Title: Hui a me ka hōʻoia ʻana o ʻelua Gap-free Reference Genomes no Xian/indica Rice e hōʻike ana i nā ʻike i ka Plant Centromere Architecture
Doi:https://doi.org/10.1101/2020.12.24.424073
Ka manawa i kau ʻia: Ianuali 01, 2021.
Institute: Huazhong Agricultural University, Kina
Nā mea waiwai
O. sativa xian/indicaʻano laiki 'Zhenshan 97 (ZS97)' a me 'Minghui 63 (MH63)
Hoʻolālā kaʻina
Heluhelu NGS + Helu HiFi + Heluhelu CLR + BioNano + Hi-C
ʻIkepili:
ZS97: 8.34 Gb（~23x）Heluhelu iā HiFi + 48.39 Gb (~131x) Heluhelu CLR + 25 Gb(~69x) NGS + 2 pūnaewele BioNano Irys
MH63: 37.88 Gb (~103x) HiFi heluhelu + 48.97 Gb (~132x) CLR heluhelu + 28 Gb(~76x) NGS + 2 BioNano Irys pūnaewele
Kiʻi 1 ʻElua mau ʻano lāʻau lapaʻau ʻole o ka laiki (MH63 a me ZS97)
2ndMaiʻa Genome2
Title: Telomere-to-telomere gapless chromosomes o ka maiʻa me ka hoʻohana ʻana i ke kaʻina nanopore
Doi:https://doi.org/10.1101/2021.04.16.440017
Ka manawa i kau ʻia: ʻApelila 17, 2021.
Ke kula: Université Paris-Saclay, Palani
Nā mea waiwai
haploid pāluaʻO Musa acuminatasppmalaccensis(DH-Pahang)
Hoʻolālā kaʻina a me ka ʻikepili:
ʻO ke ʻano HiSeq2500 PE250 + Minion/PromethION (93Gb,~200X)+ palapala ʻāina Optical (DLE-1+BspQ1)
Papa 1 Hoʻohālikelike o nā hui genome Musa acuminata (DH-Pahang).
Kiʻi 2 Musa genomes hoʻohālikelike hoʻohālikelike
3rdPhaeodactylum tricornutum genome3
Title: Telomere-to-telomere genome hui oP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Ka manawa i kau ʻia: Mei 04, 2021
Institute: Ke Kulanui o Western, Kanada
Nā mea waiwai
Phaeodactylum tricornutum(Culture Collection of Algae and Protozoa CCAP 1055/1)
Hoʻolālā kaʻina a me ka ʻikepili:
ʻO 1 Oxford Nanopore minION pūnaewele kahe + he 2×75 paired-end mid-output NextSeq 550 holo
Kiʻi 3 Kaʻina hana no ka hui genome telomere-a-telomere
4thKanaka CHM13 genome4
Title: ʻO ke kaʻina piha o kahi genome kanaka
Doi:https://doi.org/10.1101/2021.05.26.445798
Ka manawa i kau ʻia: Mei 27, 2021
Institute: National Institutes of Health (NIH), USA
Mea lako: laina kelepona CHM13
Hoʻolālā kaʻina a me ka ʻikepili:
30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), BioNano maps optical, a me Strand-seq
Papa 2 Hoʻohālikelike o GRCh38 a me T2T-CHM13 hui genome kanaka
Kuhikuhi
1.Sergey Nurk et al. ʻO ke kaʻina piha o kahi genome kanaka. bioRxiv 2021.05.26.445798; doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser et al. Telomere-a-telomere gapless chromosomes o ka maiʻa me ka hoʻohana ʻana i ke kaʻina nanopore. bioRxiv 2021.04.16.440017; doi:https://doi.org/10.1101/2021.04.16.440017
3. Daniel J. Giguere et al. Telomere-to-telomere genome hui o Phaeodactylum tricornutum. bioRxiv 2021.05.04.442596; doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Song et al. ʻO ka hui ʻana a me ka hōʻoia ʻana o ʻelua Gap-free Reference Genomes no Xian/indica Rice e hōʻike ana i nā ʻike i ka Plant Centromere Architecture. bioRxiv 2020.12.24.424073; doi:https://doi.org/10.1101/2020.12.24.424073
Ka manawa hoʻouna: Jan-06-2022