Nanopore Ka lōʻihi holoʻokoʻa

ʻO ke kaʻina hana transcriptome Nanopore kahi ala ikaika no ka hoʻopili ʻana i nā cDNA holoʻokoʻa, ʻike pololei a me ka helu ʻana i nā isoforms transcript. Hoʻolālā ʻia ka BMKCloud Nanopore Full-Length Transcriptome Pipeline e kālailai i ka ʻikepili RNA-Seq i hana ʻia ma ke kahua Nanopore e kūʻē i kahi genome kuhikuhi maikaʻi-annotated kiʻekiʻe, e hāʻawi ana i ka loiloi qualitative a me quantitative ma ka pae a me ka pae transcript. Ma hope o ka hoʻomalu maikaʻi ʻana, loaʻa nā kaʻina non-chimeric (FLNC) lōʻihi piha a hoʻopaʻa ʻia nā kaʻina consensus i ka genome kuhikuhi e wehe i nā transcripts redundant. Mai kēia pūʻulu transcript, helu ʻia ka ʻōlelo a ʻike ʻia nā genes i hōʻike ʻokoʻa ʻia a ʻike ʻia nā transcripts a hoʻopaʻa ʻia ma ka hana. Hoʻokomo pū ka pipeline i ka nānā ʻana i ka polyadenylation (APA) ʻē aʻe, ka loiloi splicing ʻē aʻe, ka nānā ʻana i ke kaʻina hana maʻalahi (SSR), ka wānana o lncRNA a me nā pahuhopu e pili ana, ka wānana o nā kaʻina coding (CDS), ka nānā ʻana o ka ʻohana gene, ka helu transcription factor, ka wānana o nā genes moʻolelo. a me ka hōʻike hana o nā transcripts.