mRNA-seq (NGS) - De novo

Hiki i ka mRNA sequencing ke hoʻopili ʻana i nā transcripts mRNA āpau i nā cell ma lalo o nā kūlana kikoʻī a he ʻenehana i hoʻohana nui ʻia i nā wahi noiʻi like ʻole. Hoʻolālā ʻia ka pipeline BMKCloud De novo mRNA-seq e kālailai i nā hale waihona puke sequencing poly-A ke loaʻa ʻole ka genome kuhikuhi. Hoʻomaka ka pipeline me ka mana maikaʻi, a ma hope ode novohui transcript a me ke koho unigene set. Hōʻike ka loiloi unigene structure i ka coding sequence (CDS) a me ka hoʻihoʻi hou ʻana i ke kaʻina maʻalahi (SSR). Ma hope mai, ʻike ʻia ka ʻikepili hōʻike ʻokoʻa i nā genes i hōʻike ʻia (DEG) ma waena o nā kūlana i hoʻāʻo ʻia, a ukali ʻia e ka annotation hana a me ka hoʻonui ʻana i nā DEG e unuhi i nā ʻike olaola.