Aloha
Hi-C he mea i hoʻolālāʻia e hopu i ka hoʻonohonoho chromosome e hoʻopili ana i ka hoʻopiliʻana i nā mea e pili ana i ka nui a me ka nui o ka heluʻana. ʻO ka ikaika o kēia mau manaʻo e manaʻoʻia e hana maikaʻiʻia me ka lōʻihi o ke kino ma nā chromosomes. No laila, hoʻohanaʻiaʻo HI-c data e alakaʻi i ka pīpī, kauoha, a me keʻano o ka hōʻiliʻiliʻana i nā haipule iā SchoomsE. Hōʻike kēiaʻenehana i kahi huiʻo Chromosome-Level Genome i ka halaʻole o ka palapala'āina genetic o kahi helu genetic. Pono kēlā me kēia genome i kahi hi-c.
Nā hiʻohiʻona lawelawe
● Holo i ka maʻi maʻi novaseq me Pe150.
● Pono ka lawelawe i nā mea i kohoʻia, ma mua o ka hoʻopiliʻana i nā maʻi nukled, e hele pū me ka formaldehyde a mālama i nā mea hana dna-protein.
● Ua pili ka hoʻokolohua Hi-c e pili ana i ka hoʻomahaʻana a me ka hopena o ka hopena o ka Sticky me Biotin, i ukaliʻia e ka piliʻana i nā pilina. A laila e hukiʻia ka DNA i lalo me nā beads storptavidin a hoʻomaʻemaʻeʻia no ka hoʻomākaukauʻana o ka hale kūʻai hale.
Loaʻa nā lawelawe lawelawe
Kaʻike o Hi-C
(Lieberman-Aiden e et al.,'Apekema hā, 2009)
●Hoʻopau i ka pono no kaʻikepili Genetic helu helu kanaka:Hi-C Rubstrated i kaʻike pono e pono ai no ka hoʻopiliʻana i ka hoʻopiʻi.
●Kālā papa hana kiʻekiʻe:e alakaʻi ana i kahi kūlana hoʻokūkū kiʻekiʻe kiʻekiʻe ma luna o 90%.
●Nā hōʻoia ikaika a me nā palapala hōʻike:Ua loaʻa iā BMKGEE ma mua o 2000 mau hihia o ka hālāwai Genome mai ka State Genome mai 1000 mauʻano likeʻole. Ma luna o 200 mau hihia i paʻiʻia he hopena accumublative accom stuff of he 2000.
●ʻO ka hui pūʻanaʻo Biinformatics Biinformatics:Me nā hale kūʻai hale a me nā polokalamu kope kope no Hi-c nā hōʻike hōʻikeʻike, ka hanaʻana i nā polokalamuʻike pilikino, a me ka hoʻihoʻiʻana.
●Kākoʻo kākoʻo:Hoʻopili kā mākou kūpaʻaʻana ma mua o ka hoʻopauʻana i kahi papa hana me kahi manawa lawelawe 3-mahina ma hope o ka lawelawe lawelawe. I kēia manawa, hāʻawi aku mākou i nā hana hahai, a me nā manaʻo e hoʻopiʻiʻia i kōkua, a me ke kau e pili ana i nā noi.
●KA MANAO KAHAKANA: Ua hoʻohana mākou i nāʻikepili he nui e hana ai i nāʻano likeʻole me nāʻano mea i hōʻikeʻia a hana i nā hana noiʻi he nui.
Nāʻikepili lawelawe
| Hale kūʻai hale kūʻai | Serenencing hoʻolālā | ʻO kaʻikeʻikepili i hōʻikeʻia | Honua mālamalama |
| Hi-c Pūnaewele | Manaʻo maoli'ovaseq Pe150 | 100x | Q30 ≥ 85% |
Nā koi koi
| Kahi | Ka nui |
| Nā holoholona holoholona | ≥ 2 g |
| ʻO nā Musole holoholona | |
| ʻO ke koko mammania | ≥ 2 ml |
| ʻO ke koko wai / iʻa | |
| Kūkulu - lau hou | ≥ 3 g |
| Nā cell creol | ≥ 1x107 |
| INSICT | ≥ 2 g |
E holo ka lawelawe lawelawe
Hoʻolālā hoʻokolohua
Nā Hoʻounaʻana
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
1) raw data qc
2) Hi-C PERS QC: Kuhi o nā hui H-C
3) Hi-C SPEPE: Clustering o nā huiʻana i nā hui, hahaiʻia e ka hoʻonohonohoʻana i ka hoʻonohonohoʻana i loko o kēlā me kēia hui
4) Hi-c loiloi
Hi-c puke qc
Aloha-C Guart - Statistics
ʻO ka loiloi post-State - Handmap o ka hōʻailona hōʻailona ma waena o nā pa
Eʻimi i nā mea holomua i hoʻomaikaʻiʻia e BMKGNET o ka lawelaweʻo BMKGENE BMKGNEY ma o kahi hōʻiliʻili o nā paʻi.
Tian, t. et al. (2023) 'Gonome hui a me keʻano Genetic o kahi hana hilahila mai kahi germant Doi: 10.1038 / s41588-023-01297-y.
Wang, zl et al. (2020) 'kahi hui chromosome-scarosome-scale o ka meli asia asia asia asian 52414040. Doi: 10.3389 / Fgene.2020.2020.00279 / Bibtex.
Zhang, f. et al. (2023)ʻO ka hoʻolahaʻana o Truolut o Tropane Alkaloid bioysnthesis ma ka nānāʻana iʻelua mau genomes Doi: 10.10338 / S4147-023-37133-4.
Zhang, x. et al. (2020) 'ʻO Genomes o ka lāʻau banyan a me ka pllinitor e hāʻawi i nā meaʻike i loko o ka coevolution', cell, pp. " Doi: 10.1016 / J.CEll.202099.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.09.0903
