Hoʻopili Chromatin ma muli o Hi-C
Nā hiʻohiʻona lawelawe
● Ka hoʻonohonoho ʻana ma Illumina NovaSeq me PE150.
● Pono ka lawelawe i nā laʻana kiko, ma kahi o nā ʻakika nucleic i unuhi ʻia, e hoʻokuʻi me ka formaldehyde a mālama i nā pilina DNA-protein.
● Aia ka hoʻokolohua Hi-C i ka hoʻopaʻa ʻana a me ka hoʻopau ʻana i ka hoʻoponopono ʻana i nā wēlau paʻa me ka biotin, a ukali ʻia e ka circularization o nā hopena blunt i ka wā e mālama ai i nā pilina. A laila huki ʻia ka DNA me nā streptavidin beads a hoʻomaʻemaʻe ʻia no ka hoʻomākaukau ʻana i ka waihona.
Pono lawelawe
●Hoʻolālā Enzyme hoʻopaʻa maikaʻi loa: e hōʻoia i ka hana kiʻekiʻe o ka Hi-C ma nā ʻano ʻano like ʻole a hiki i ka 93% mau pilina pili pono.
●ʻIke Nui a me nā moʻolelo hoʻolaha:He ʻike nui ʻo BMKGene me >2000 Hi-C sequencing projects mai 800 mau ʻano ʻano like ʻole a me nā patent like ʻole. ʻOi aku ma mua o 100 mau hihia i paʻi ʻia me kahi kumu hopena o ka 900.
●Pūʻulu Bioinformatics akamai loa:me nā patent i loko o ka hale a me nā kope kope polokalamu no nā hoʻokolohua Hi-C a me ka nānā ʻana i ka ʻikepili a me kahi lako polokalamu ʻike pilikino i kūkulu ʻia.
●Kākoʻo ma hope o ke kūʻai ʻana:ʻO kā mākou kūpaʻa e ʻoi aku ma mua o ka hoʻopau ʻana o ka papahana me kahi 3 mau mahina ma hope o ke kūʻai aku ʻana. I kēia manawa, hāʻawi mākou i ka hahai ʻana o ka papahana, kōkua hoʻoponopono, a me nā hālāwai Q&A e hoʻoponopono i nā nīnau pili i nā hopena.
●Hōʻikeʻike piha: hoʻohana mākou i nā ʻikepili he nui no ka hoʻopuka pono ʻana i nā genes me nā ʻano like ʻole i ʻike ʻia a hana i ka loiloi hoʻonui kūpono, e hāʻawi ana i nā ʻike no nā papahana noiʻi lehulehu.
Nā kikoʻī lawelawe
| Hale Waihona Puke | Hoʻolālā Sequencing | Hoʻopuka ʻia ka ʻikepili i manaʻo ʻia | Hoʻoholo hōʻailona Hi-C |
| Hale waihona Hi-C | Illumina PE150 | Loko Chromatin: 150x TAD: 50x | Loop Chromatin: 10Kb TAD: 40Kb |
Nā Koina lawelawe
| ʻAno laʻana | Ka nui i makemakeia |
| ʻiʻo holoholona | ≥2g |
| Koko holookoa | ≥2mL |
| nā haʻihaʻi | ≥1g |
| Mea kanu- ʻōpiopio | 1g/aliquot, 2-4 aliquote i paipai ʻia |
| Nā Pūnaehana Moʻomeheu | ≥1x107 |
Loaʻa i ka ʻikepili ma lalo nei:
● QC ʻikepili maka;
● Palapala palapala a me ka waihona Hi-C QC: nā hui pilina kūpono a me Interaction Decay Exponents (IDEs);
● Genome-wide Interaction Profiling: cis/trans analysis and Hi-C interaction map;
● Ka nānā 'ana o ka pu'unaue A/B;
● Hoʻomaopopo i nā TAD a me nā puka lou chromatin;
● Ke kālailai ʻokoʻa i nā mea hoʻolālā chromatin 3D ma waena o nā laʻana a me ka hōʻike kikoʻī hana o nā genes pili.
ʻO Cis a me ka hāʻawi like ʻana
Heatmap o ka pilina chromosomal ma waena o nā laʻana
ʻO ka māhele Genome-ākea o nā keʻena A/B
Hoʻohelehele genome-ākea o nā puka lou chromatin
ʻIke ʻana i nā TAD
E ʻimi i nā holomua noiʻi i hoʻomaʻamaʻa ʻia e BMKGene's Hi-C sequencing services ma o ka hōʻiliʻili ʻana o nā puke.
Meng, T. et al. (2021) 'Hoʻomaopopo ka hoʻohālikelike ʻana i nā multi-omics analysis i ka CA2 ma ke ʻano he pahuhopu hou no ka chordoma',Neuro-Oncology, 23(10), pp. 1709–1722. doi: 10.1093/NEUONC/NOAB156.
Xu, L. et al. (2021) 'O ka disorganization 3D a me ka hoʻonohonoho hou ʻana o ka genome e hāʻawi i nā ʻike i ka pathogenesis o NAFLD e ka hui ʻana o Hi-C, Nanopore, a me RNA sequencing',ʻO Acta Pharmaceutica Sinica B, 11(10), pp. 3150–3164. doi: 10.1016/J.APSB.2021.03.022.
