De novo fungal genome genome
Hāʻawiʻo BMKGEE i nā hoʻonā Venetalile no nā genomes fungal, e pono ai e mālama i nā noiʻi noiʻi. Hoʻohanaʻo ia i ka'ōlelo i hiki ke heluheluʻia i ka'ōleloʻana i ka fequina i ka hanauna o kahi braft genome. ʻO nā mea pōkole a me ka lōʻihi a me ka lōʻihi o ka heluheluʻana e hoʻohana ai i ka inoaʻo Nanopore a iʻole macbio i hui pūʻia no kahi genome hou aku i ka genome hou. Eia kekahi, hoʻohui hou iā Hi-C Sequencing i nā hiki
Nā hiʻohiʻona lawelawe
Me nā koho kohoʻekolu e koho ai mai ke kumu i makemakeʻia ma keʻano o keʻano o Genome.
● Ke kohoʻana i nā koho Genome Genome:
● E kohoʻo Genome Genome Genome:
Nā hoʻokolohua kūlanaʻo Stanme:'Ollumina Novaseq Pe150.
Genome Stors: Pacbio Revoino
● Kohu-chromosome-pae gungal genome:
Nā hoʻokolohua kūlanaʻo Stanme:'Ollumina Novaseq Pe150.
Genome Stors: Pacbio Revoino
Ka hoʻohālikelikeʻana me ka hui hi-c hui.
Loaʻa nā lawelawe lawelawe
●Loaʻa i nā hoʻolālā senquing i loaʻa: No nā pahuhopu noiʻi likeʻole a me nā koi o nā pono o Genome
●ʻO ka piha o bioinformatics piha:E pili ana kēia i ka hālāwai hālāwaiʻo Genome a me ka wānana o nā hanana genomple he nui, hana i ka hoʻonāukiukiʻana, a me ka hoʻopiʻiʻana.
●ʻO kaʻike nui: Me nā mea he 12,000 mau makahikiʻo Microbial, e lawe mai iā ia ma luna o kahi mau makahiki, kahi kūlana kiʻekiʻe loa, a me ke kākoʻoʻana i ke kiʻi.
●Kākoʻo kākoʻo:Hoʻopili kā mākou kūpaʻaʻana ma mua o ka hoʻopauʻana i kahi papa hana me kahi manawa lawelawe 3-mahina ma hope o ka lawelawe lawelawe. I kēia manawa, hāʻawi aku mākou i nā hana hahai, a me nā manaʻo e hoʻopiʻiʻia i kōkua, a me ke kau e pili ana i nā noi.
Nāʻikepili lawelawe
| Hoʻokaumi | Serenencing hoʻolālā | Honua mālamalama |
| Draft genome | ʻO Ihemina Pe150 100x | Q30≥85% |
| Maikaʻi genome | GENONOM KĀNUI SITESE: ELLUMINA Pe150 50 x Spice: Pacbio Hifi 30x a iʻole Nanopore 100x | contig n50 ≥14Mb (pacbio unicelular) contig n50 ≥2mb (ont unicelular) concig n50 ≥500kb (kekahi) |
| Chromosome-pae genome | GENONOM KĀNUI SITESE: ELLUMINA Pe150 50 x Spice: Pacbio Hifi 30x a iʻole Nanopore 100x Hi-c hālāwai 100x | E hoʻokūkū i ka hoʻohuiʻana i ka hoʻohuiʻana> 90%
|
Nā lawelawe no ka lawelawe
| Ka Manaʻo (NG / μl) | Ka nui nui (μg) | Nui (μl) | Od260 / 280 | Od260 / 230 | |
| PACBio | ≥20 | ≥2 | ≥20 | 1.7-2.2 | ≥1.6 |
| Nana'Ooke | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| ʻO Malomina | ≥1 | ≥0.06 | ≥20 | - | - |
Unicellar fungus: ≥3.5x1010 manu
Macro fungus: ≥10 g
E holo ka lawelawe lawelawe
Nā Hoʻounaʻana
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
Komo i ka loiloi ma lalo nei:
GENOME Story:
- ʻO ka mālamaʻana i ka mana o kaʻikepili
- ʻO Genome Kuhi:ʻO ka nui, heterozygossity, nā mea hou
ʻO ka hālāwai hālāwaiʻo Genome:
- ʻO ka mālamaʻana i ka mana o kaʻikepili
- De nomvoKāhea
- ʻO Genome Commonitentis messagesis: wānana o nā CD a me nā mea he nui
- Hoʻolaha i kahi annotation me nāʻikepili lehulehu (hele, kegg, etc.) a me nā waihona waiwai
Hi-C Huipu:
- Hi-C waihona loiloi.
- E hoʻohālikelike ana i nā clustering alchoring, kauoha a me ka orrienting
- Ka loiloi o Hi-C Pai: E pili ana ma ke kuhikuhiʻana o GenoMe a me ka pākaukau wela
Genome Survey: K-me ka hoʻokaʻawaleʻana
Genome Speph: Gene Wane Homologous Annologous Ancoation (NR Data Database)
Genome Sisp Session: Hana i ka hana GenoNotation (hele)
Eʻimi i nā mea holomua i hoʻomaikaʻiʻia e BMKGEMEʻo BMKDEM o GENMOMEME o GENMOMEM ma kahi o ka hōʻiliʻiliʻana o nā paʻi.
Hao, j. et al. (2023) 'hoʻohui hoʻohuiʻana o Omic e hōʻike ana i ka onotus bushomus i loko o ka'ōpū ma lalo o nā kūlana i lalo o nā kūlana i laloBmc genoics, 24 (1), PP. 1-12. Doi: 10.1186 / S12864-023-093-09656-Z / 3.
L, L. Et ALT AL. (2023) 'genome sequence e hōʻike ana i ka Evolution a me Pathogenic i nā hana o ka What Sharp Prizotton ChizotnoniaʻO ka puke CROP, 11 (2), PP. 405-416. Doi: 10.1016 / J.CJ.20222.07.024.
Zhang, H. et al. (2023) 'ʻO nā kumuwaiwai Genome noʻehā mau kiʻi clarireedia e hana ana i ke kālā kālā e pili ana i nā turfgrasses turfgrasses'.Mea kanu maʻi, 107 (3), pp. 929-934. Doi: 10.1094 / PDIS-08-22-1921-A
Zhang, ss et al. (2023) 'genetic a me nā hōʻike molecular a me nā molecular i hōʻikeʻia i kahi'ōnaehana tetrapolar ma ka mushopsNūpepa o ka fungi, 9 (10), p. 959. Doi: 10.3390 / Jof9100959 / S1.
