Piha piha mrna seequence-Nanopore
Nā hiʻohiʻona
● hopuʻana iā Poly-a i ukaliʻiaʻo Mrna Synthesis a me ka hoʻomākaukauʻana
● Sequenting o nā kope piha piha
● Nā Manaʻo BiintoChetic ma muli o ke kuleana i kahiʻano e pili ana iā Genome
●ʻAʻole paʻa ka nānāʻana o BiintoChetic ma Gene lāuaʻo IsOform
Loaʻa nā lawelawe lawelawe
●Ka heluʻana o ka hōʻike ma ka pae o ka isoform: KAʻIʻO ACTERDING ID a me ka hōʻikeʻana i ka hōʻike hōʻikeʻike, weheʻoleʻia e hiki ke masked i ka wā e nānā ai i ka hōʻikeʻana o keʻano hana
●E hoʻemiʻia nā koiʻikepili:Hoʻohālikelike i nā mea hou aku o ka makahiki o ka hanauna ma hope o nā makahiki he nui.
●ʻOi aku ka pololei o ka hōʻike hōʻike: ma keʻano he nui a me keʻano
●Kaʻikeʻana i kaʻike kākau kākau inoa hou: FORTODALY POLYDENYLATALY, Fusion Genes a me Lcnrna a me kā lākou mau getes
●ʻO kaʻike nui: Ua lawe mai kā mākou hui i kahi waiwai o kaʻike i kēlā me kēia papahana, ua hoʻopauʻia ma luna o 850 nanopore piha piha i nā papa inoa lōʻihi.
●Kākoʻo kākoʻo: Ke hoʻonui nei kā mākou hana i waho ma mua o ka hoʻopauʻana i kahi papa hana me kahi 3-mahina ma hope o ka lawelawe lawelawe. I kēia manawa, hāʻawi aku mākou i nā hana hahai, a me nā manaʻo e hoʻopiʻiʻia i kōkua, a me ke kau e pili ana i nā noi.
Nā koi koi a me ka laweʻana
| Puka Hanau | Serenencing hoʻolālā | Ua'ōleloʻia nāʻikepili | Honua mālamalama |
| Poly i kauʻia | Hoʻomālamalama pe150 | 6/12 gb | ʻO ka helu kūpono kūpono: Q10 |
Nā Manaʻo Manaʻo:
Nucletides:
| SCA. (NG / μl) | Ka nui (μg) | ʻO Menu ka hoʻomaʻemaʻe | Huakai |
| ≥ 100 | ≥,0 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Palenaʻole aʻaʻohe protein a iʻole dna conceation i hōʻikeʻia ma ka gel. | No nā mea kanu: Rin≥7.0; No nā holoholona: ROC≥7.5; 5.0≥28s / 18s. kaupalena a iʻole he kumu kūʻai baseline |
● Nā mea kanu:
All, stem or petal: 450 mg
Lau a iʻole hua: 300 mg
Nā hua: 1.2 g
● nā holoholona:
Naʻau a iʻole nā'ōpū: 300 mg
Viscera a lolo paha: 240 mg
Muscle: 450 mg
Iwi, lauoho lauoho aʻili paha: 1g
● artoropods:
Nā mea pepeke: 6g
Crustaca: 300 mg
● koko āpau: 1 Tube
● nā cell: 106 manu
Hōʻikeʻia ka hoʻounaʻana
Nā pahu: 2 ML Cetrifuge Tube (Tin Foilʻaʻole i aʻoʻia)
Sample Labelning: Kumupa + Ka Hoʻolau'Awele iā A1, A2, A3; B1, B2, B3.
Hoʻouna:
1.ʻO ka maloʻo maloʻo: Pono nā specles i hoʻopaʻaʻia i nāʻeke a kanuʻia ma ka maloʻo maloʻo.
2. E hiki ke maloʻo nā tubes: Hiki ke maloʻoʻo RNA i ka smabillization rena ma rna stabillization (e like me rnastable®)
E holo ka lawelawe lawelawe
Nucletides:
Nā Hoʻounaʻana
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
E holo ka lawelawe lawelawe
Tissue:
Hoʻolālā hoʻokolohua
Nā Hoʻounaʻana
RNA extraction
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
● Ke kaʻina hanaʻikepili
● Kuhi palapala
● Nā Splincing
●ʻO ka hōʻike hōʻikeʻike i ka pae inf a me ke kiʻekiʻe
●ʻO ka hōʻike hōʻikeʻokoʻa
● Hana i ka hana a me ka hoʻonuiʻana (nā mea hana a me nā dets a me nā dets)
ʻO ka nānāʻana i ka nānāʻana
ʻO keʻano hana likeʻo Polyadylation (APA)
lncrna wānana
ʻO ka antation o nā hua'ōlelo novel
Clustering o nā dets
ʻO nā pūnaewele protein-protein i loko o nā degs
Eʻimi i nā mea holomua i hoʻonuiʻia e BMKDEREʻo BMKGEN's Nanoperne piha piha o MRNA SEQUENCING lawelawe ma kahi hōʻiliʻili o nā paʻi.
Gong, b. et al. (2023) '' OPIGENTIC a me ka EPIGNITAL O KA HUI KINOS KINOSOCT MA KE ANA I GLIODICE MA GIOGOSE MA GIOMOSE MA GIOGOCS MA GIOMOCTIES. 50 (6), PP. 422-433. Doi: 10.1016 / J.JGGG.2023.01.008.
He, z. et al. (2023) '' piha piha piha o ka lymmphocytes e pane aku ai i ka Ifmune i ka flounder) ' 108636. Doi: 10.1016 / J.fsi.2023.108636.
Ma, y. et al. (2023) 'hoʻohālikelike hoʻohālikelike o Pacbio a ont rna sequing e ala no ka nemopilema no ka nemopilema nomopics, 115 (6), p. 11009. Doi: 10.1016 / J.ygeno.2023.11009.
YU, D. Et Al. (2023) 'Nano-seq loiloi i hōʻikeʻia ma waena o nā mea hana likeʻole ma waena o nā exosoms a me nā microsic Doi: 10.1186 / s13287-023-03491-5 / papa / 6.
