Eukaryotic mRNA Analysis (Reference based and de novo options available)
Ke hoʻohana nei kēia pipeline i ka ʻikepili NGS RNA-Seq ma ke ʻano he hoʻokomo a hoʻopuka i nā hopena mai nā hoʻopaʻa ʻana he nui i lalo me ka ʻaʻole i kaupalena ʻia:de novoka palapala hōʻike pūnaewele, ka nānā ʻana i ka hoʻopili ʻana i nā ʻano like ʻole, ka nānā ʻana i ka ʻōlelo ʻokoʻa, ka wehewehe hana a me ka nānā ʻana i ka hoʻonui.
ʻIke RNA lōʻihi ʻole coding
ʻO nā RNA non-coding lōʻihi (lncRNA) he mau transcript non-coding me ka lōʻihi ma mua o 200 nt a ʻike ʻia e pāʻani i nā kuleana ma ka hui chromatin a me ka hoʻoponopono. Ua hoʻoikaika nā ʻenehana sequencing kiʻekiʻe a me ka bioinformatic i ko mākou ʻike ʻana i nā kaʻina lncRNA a me ka ʻike hoʻonohonoho e ʻike ai i nā lncRNA me nā hana hoʻoponopono koʻikoʻi. Hāʻawi kēia pipeline i ka loiloi lncRNA ma kahi o nā loiloi i ʻōlelo ʻia ma ka pipeline Eukaryotic mRNA Analysis.
16S/18S/ITS amplicon sequencing
Ua hoʻomohala ʻia ka pipeline ʻano ʻano like ʻole microbial Amplicon Sequencing ma muli o nā makahiki o ka ʻike i ka loiloi papahana microbial diversity. Aia i loko o ka pipeline ka loiloi kumu maʻamau, e uhi ana i ka ʻike loiloi kumu o ka noiʻi microbial o kēia manawa a me ka loiloi pilikino. He waiwai a piha ka hōʻike hōʻike, me ke koho e hana i nā ʻano loiloi pilikino like ʻole. Eia hou, hiki ke hoʻololi ʻia nā laʻana a me nā pūʻulu ma ka lele no ka hana maʻamau a me ka mana.
ʻIkepili Metagenomics pū pana
Hoʻohana ka Shotgun Metagenomic Analysis pipeline i ka ʻikepili NGS mai nā mea genomic hui ʻia i lawe ʻia mai nā laʻana kaiapuni. Hāʻawi ʻia nā kānana i ka ʻike kikoʻī e pili ana i ka ʻokoʻa a me ka nui o nā ʻano, ka hoʻonohonoho ʻana o ka heluna kanaka, ka pilina phylogenetic, nā genes hana, a me nā ʻoihana hoʻoponopono me nā mea kaiapuni.
ʻIkepili ʻokoʻa NGS-WGS
ʻO ka NGS-WGS Variant Analysis kahi pipeline ʻike ʻokoʻa i hoʻohui ʻia e hana ana i ka mana o ka maikaʻi o ka ʻikepili, ka hoʻonohonoho ʻana i ke kaʻina, ka hōʻike ʻana, a me ka nānā ʻana o ka mutation gene. Hoʻopili ka pipeline i nā hana maikaʻi loa GATK no ka ʻike SNP a me InDel a hoʻohana iā Manta no ke kelepona ʻokoʻa.
ʻO ke aʻo ʻana o ka hui ʻo Genome Wide Association (GWAS)
ʻO ka pipeline GWAS kahi kānana i lalo e lawe ana i nā faila VCF i hana mua ʻia a me nā ʻikepili phenotype e pili ana no kahi cohort o nā kānaka ma ke ʻano he hoʻokomo. Ke hoʻohana nei i nā ʻano helu helu kikoʻī, manaʻo ʻo GWAS e wehe i nā ʻokoʻa nucleotide ākea genome i hoʻopili ʻia me nā ʻokoʻa phenotypic. He kuleana koʻikoʻi ia i ka ʻimi ʻana i nā genes hana e pili ana i nā maʻi paʻakikī o ke kanaka a me nā ʻano paʻakikī o nā mea kanu a me nā holoholona.
Nānā Hoʻokaʻawale Nui (BSA)
Hoʻopili ka loiloi BSA i ka hui ʻana i nā poʻe me nā ʻano phenotypic koʻikoʻi mai kahi heluna hoʻokaʻawale. Ma ka hoʻohālikelike ʻana i ka loci ʻokoʻa ma waena o nā laʻana i hoʻohui ʻia, ʻike koke kēia ala i nā māka molekala pili pili e pili ana i nā genes. Hoʻohana nui ʻia i ka palapala ʻāina genetic o nā mea kanu a me nā holoholona, he mea waiwai ia no ka hoʻoulu ʻana i kōkua ʻia.
ʻIkepili Genetics Evolutionary
Hoʻohana ka Evolutionary Genetics Analysis workflow i ka ʻike nui o BMKGENE i nā papahana evolution genetic a loaʻa pū me ke kūkulu ʻana i nā kumulāʻau phylogenetic, linkage disequilibrium analysis, genetic diversity assessment, selective sweep identification, kinship analysis, principal component analysis, and population structure characterization.
