TGuide Smart Magnetic Plant RNA Kit
Helu helu / puʻupuʻu
| Cat. ʻAʻole | ID | Ka helu o nā preps |
| 4993552( pahu pahu) | DP662-DE | 48 |
ʻO ke alakaʻi koho ʻana i ka reagent no TGuide S16
| Nuclei Acid | ʻAno Laʻana | Inoa Huahana | Ka nui o ka paʻi (preps) pahu pahu/ pāpaʻi | Cat no. pahu pahu |
| DNA | Pepa Holoholona | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Meakanu & Hua | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Ka lepo/Stool | TGuide Smart Soil/Stool DNA Kit | 48 | 4993549 |
| DNA | Nā huahana Gel & PCR | ʻO TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Koko holookoa | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Maloo, etc | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Koko/Cell/Tssue | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Meakanu & Hua | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | ʻO nā wai me ka ʻole o ke kelepona (Serum, etc.) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Hoʻolauna huahana
E hoʻomaʻemaʻe i ka RNA piha kiʻekiʻe, kiʻekiʻe-maʻemaʻe, kiʻekiʻe mai ke koko/cell/tissue.
ʻAʻohe mea ʻawaʻawa e like me ka phenol/chloroform
Loaʻa ka maʻemaʻe kiʻekiʻe o ka RNA i loaʻa a hiki ke hoʻohana pololei ʻia no ka ʻike ʻana i ka chip, ka hoʻoiho kiʻekiʻe-throughput a me nā hoʻokolohua ʻē aʻe.
Nā hiʻohiʻona
Maʻalahi loa e hoʻohana
ʻAʻohe hana paipu keu.
Hiki ke hoʻololi i ka leo elution e like me ka makemake.
Pono ka hoʻokomo a me ka hana i ka hoʻomaʻamaʻa liʻiliʻi. Me nā polokalamu i hoʻokomo mua ʻia, wehe i ka pahu pahu, koho i ka protocol a holo i kāu hoʻokolohua. Ua mākaukau nā papahana e hoʻohana, a hiki ke hoʻololi ʻia.
ʻO nā reagents i hoʻouka mua ʻia a me nā mea hoʻopau hoʻopau ʻia.
Hoʻopau ʻia ka mālama ʻana i ke kemika i ka nui loa e hōʻoia i ka palekana o ka hana.
Ka hopena Demo
Huapalaoa huina RNA unuhi
Laʻana nui: 100 mg
Laʻana pretreatment: wili me ka wai nitrogen a haʻahaʻa wela wela homogenizer lapaʻau
ʻO ka manaʻo o ka Agarose gel: 2%(TAE)
Hoʻouka nui: 2 μl
Hoailona: Marker IV, TIANGEN
Ka hopena hoʻokolohua: me ka hoʻohana ʻana iā TGuide S16 i loaʻa me ka reagent 4993552 e unuhi aunoa i ka huina RNA o nā aʻa palaoa i loaʻa ka hua maikaʻi a me ka maʻemaʻe kiʻekiʻe. Ma kēia hoʻokolohua, ma kahi o 20 μg o ka nucleic acid i unuhi ʻia mai 100 mg aʻa palaoa, me OD260/OD280 ma kahi o 2.0~2.1, a me OD260/OD230＞2.0.
Huina RNA unuhi mai na mea kanu like ole
Laʻana nui: 100 mg
Laʻana pretreatment: haʻahaʻa-mehana homogenizer
ʻO ka manaʻo o ka Agarose gel: 1% (TAE)
Hoʻouka nui: 1 μl
M: Marker III, TIANGEN
1-4: hua kulina 5-8: lau kulina 9-12: aʻa kāpeti 13-16: soya
Ua unuhi ʻia nā laʻana mua ʻelua e ka pahu hōʻiliʻili spin-column a ua unuhi ʻia nā laʻana ʻelua hope e TGuide S16.
Ka hopena hoʻokolohua: ʻoi aku ka like a i ʻole ʻoi aku ka maikaʻi o ka loaʻa ʻana o ka mea kanu RNA e TGuide S16 me ka reagent 4993552 ma mua o ka pahu hōʻiliʻili spin-column. ʻO ka maʻemaʻe a me ka pololei keʻano like. No laila, hiki ke hoʻohana ʻia ʻo TGuide S16 no ka unuhi ʻana i ka RNA a pau o ka mea kanu ma ke pani ʻana i ka hopena hoʻoneʻe e pili ana i ka spin-column.
