TGuide Smart Blood/Cell/Tissue RNA Kit
Helu helu / puʻupuʻu
| Cat. ʻAʻole | ID | Ka helu o nā preps |
| 4995043(pāpā) | DP661-E | 96 |
| 4993551(pahu pahu pahu) | DP661-DE | 48 |
ʻO ke alakaʻi koho ʻana i ka reagent no TGuide S16
| Nuclei Acid | ʻAno Laʻana | Inoa Huahana | Ka nui o ka paʻi (preps) pahu pahu/ pāpaʻi | Cat no. pahu pahu |
| DNA | Pepa Holoholona | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Meakanu & Hua | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Ka lepo/Stool | TGuide Smart Soil/Stool DNA Kit | 48 | 4993549 |
| DNA | Nā huahana Gel & PCR | ʻO TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Koko holookoa | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Maloo, etc | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Koko/Cell/Tssue | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Meakanu & Hua | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | ʻO nā wai me ka ʻole o ke kelepona (Serum, etc.) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Hoʻolauna huahana
E hoʻomaʻemaʻe i ka RNA piha kiʻekiʻe, kiʻekiʻe-maʻemaʻe, kiʻekiʻe mai ke koko/cell/tissue.
ʻAʻohe mea ʻawaʻawa e like me ka phenol/chloroform
Loaʻa ka maʻemaʻe kiʻekiʻe o ka RNA i loaʻa a hiki ke hoʻohana pololei ʻia no ka ʻike ʻana i ka chip, ka hoʻoiho kiʻekiʻe-throughput a me nā hoʻokolohua ʻē aʻe.
Nā hiʻohiʻona
Maʻalahi loa e hoʻohana
ʻAʻohe hana paipu keu.
Hiki ke hoʻololi i ka leo elution e like me ka makemake.
Pono ka hoʻokomo a me ka hana i ka hoʻomaʻamaʻa liʻiliʻi. Me nā polokalamu i hoʻokomo mua ʻia, wehe i ka pahu pahu, koho i ka protocol a holo i kāu hoʻokolohua. Ua mākaukau nā papahana e hoʻohana, a hiki ke hoʻololi ʻia.
ʻO nā reagents i hoʻouka mua ʻia a me nā mea hoʻopau hoʻopau ʻia.
Hoʻopau ʻia ka mālama ʻana i ke kemika i ka nui loa e hōʻoia i ka palekana o ka hana.
Ka hopena Demo
Ua hoʻohana ʻia ʻo Agilent 2100 no ka ʻike pono.
Kumu kumu: CD-1 ʻiole
Ke kūlana laʻana: ke koko holoʻokoʻa hou
Nui laʻana: 200 μl
Ka hopena hoʻokolohua: ʻo ka huina RNA i lawe ʻia mai ke koko e TGuide S16 i hoʻolako ʻia me ka reagent 4993551 he hua maikaʻi a me ka maʻemaʻe kiʻekiʻe, a hiki ke hoʻokō i nā pono o lalo.
kiʻekiʻe-throughput sequencing.
Kumu kumu: CD-1 ʻiole
Hoʻouka nui: 1 μl. Ua hoʻoheheʻe ʻia ke akepaʻa i 5 mau manawa a hoʻouka ʻia.
ʻO ka manaʻo o ka Agarose gel: 1%. Electrophoresis no 20 min ma 6 V / cm;
M: TIANGEN Marker III 1-4: naʻau 5-8:
ate 9-12: akemāmā 13-16: ʻākena
Ua unuhi ʻia nā laʻana mua ʻelua o kēlā me kēia ʻiʻo e ka pahu hōʻiliʻili spin-column, a ʻo nā mea hope ʻelua i unuhi ʻia e TGuide S16.
ʻO ka hopena hoʻāʻo: ʻo ka nui o nā holoholona holoholona RNA i unuhi ʻia e TGuide S16 i lako me ka reagent 4993551 he kiʻekiʻe ka hua a me ka maʻemaʻe, ua like ia me ka RNA i unuhi ʻia e ke kaʻina hoʻoheheʻe spin-column based extraction protocol. Hoʻokahi like ka maʻemaʻe, me OD260/OD280 a puni 2.0 ~ 2.1 a me OD260/OD230 > 2.0. I ka hopena, hiki ke hoʻohana ʻia ʻo TGuide S16 no ka unuhi ʻana i ka huina RNA mai nā ʻiʻo holoholona ma ke pani ʻana i ka hopena hoʻoneʻe e pili ana i ka spin-column.
