TGuide Smart Magnetic Plant DNA Kit
Helu helu / puʻupuʻu
| Cat. ʻAʻole | ID | Ka helu o nā preps |
| 4993548(kāleka) | DP607-DE | 48 |
ʻO ke alakaʻi koho ʻana i ka reagent no TGuide S16
| Nuclei Acid | ʻAno Laʻana | Inoa Huahana | Ka nui o ka paʻi (preps) pahu pahu/ pāpaʻi | Cat no. pahu pahu |
| DNA | Pepa Holoholona | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Meakanu & Hua | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Ka lepo/Stool | TGuide Smart Soil/Stool DNA Kit | 48 | 4993549 |
| DNA | Nā huahana Gel & PCR | ʻO TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Koko holookoa | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Maloo, etc | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Koko/Cell/Tssue | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Meakanu & Hua | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | ʻO nā wai me ka ʻole o ke kelepona (Serum, etc.) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Hoʻolauna huahana
Hoʻohana ākea: He kūpono ia no nā ʻiʻo mea kanu like ʻole, ʻoi aku ka polysaccharide- a i ʻole nā mea kanu waiwai polyphenol.
Palekana a ʻaʻohe mea ʻawaʻawa: ʻAʻohe mea ʻawaʻawa ʻawaʻawa e like me ka phenol/chloroform.
Maʻemaʻe kiʻekiʻe: He maʻemaʻe kiʻekiʻe ka DNA i loaʻa a hiki ke hoʻohana pololei ʻia no ka ʻike ʻana i ka chip, ka hoʻopili kiʻekiʻe-throughput a me nā hoʻokolohua ʻē aʻe.
Nā hiʻohiʻona
Maʻalahi loa e hoʻohana
ʻAʻohe hana paipu keu.
Hiki ke hoʻololi i ka leo elution e like me ka makemake.
Pono ka hoʻokomo a me ka hana i ka hoʻomaʻamaʻa liʻiliʻi. Me nā polokalamu i hoʻokomo mua ʻia, wehe i ka pahu pahu, koho i ka protocol a holo i kāu hoʻokolohua. Ua mākaukau nā papahana e hoʻohana, a hiki ke hoʻololi ʻia.
ʻO nā reagents i hoʻouka mua ʻia a me nā mea hoʻopau hoʻopau ʻia.
Hoʻopau ʻia ka mālama ʻana i ke kemika i ka nui loa e hōʻoia i ka palekana o ka hana.
Ka hopena Demo
ʻO Genomic DNA unuhi o ka ʻuala
Laʻana nui: 100 mg
Laʻana ma mua o ka lapaʻau ʻana: wili ʻia me ka nitrogen wai a i ʻole homogenizer kiko
ʻO ka manaʻo o ka Agarose gel: 2% (TAE)
Hoʻouka nui: 2 μl
Māka: D15000, TIANGEN S16-01, S16-02 a me S16-03 hōʻike i nā hoʻokolohua like ʻole o ka unuhi ʻana i ka DNA genomic mea kanu ma o ka hoʻohana ʻana i 3 TGuide S16 Nucleic Acid Extractors i ka manawa like.
He Hui: he brand kaulana
ʻO ka hopena hoʻokolohua: me ka hoʻohana ʻana iā TGuide S16 me ka reagent 4993548 e unuhi ʻakomi i ka DNA genomic o nā ʻuala i loaʻa i ka hua maikaʻi a me ka maʻemaʻe kiʻekiʻe, ʻo ia ka mea e like me ka maikaʻi o ka huahana unuhi o nā pahu hōʻiliʻili spin-column hoʻokūkū kaulana. Ma kēia hoʻokolohua, ua unuhi ʻia ma kahi o 11 μg o ka waikawa nucleic mai 100 mg mau ʻuala, me OD260/OD280 a puni i 1.8~1.9, a me OD260/OD230＞2.0.
ʻO Genomic DNA unuhi o ka lau soya
Laʻana nui: 100 mg
Laʻana ma mua o ka lapaʻau ʻana: wili ʻia me ka nitrogen wai a i ʻole homogenizer kiko
ʻO ka manaʻo o ka Agarose gel: 1% (TAE)
Hoʻouka nui: 2 μl
Hoailona: D15000, TIANGEN
ʻO ka hopena hoʻokolohua: me ka hoʻohana ʻana iā TGuide S16 me ka reagent 4993548 e unuhi ʻakomi i ka DNA genomic o nā lau soybean i loaʻa i ka hua maikaʻi a me ka maʻemaʻe kiʻekiʻe. Ma kēia hoʻokolohua, ma kahi o 13μg o ka nucleic acid i unuhi ʻia mai 100 mg lau soybean, me OD260/OD280 ma kahi o 1.8~1.9, a me OD260/OD230＞2.0.
ʻO Genomic DNA unuhi o nā aʻa palaoa
Laʻana nui: 100 mg
Laʻana ma mua o ka lapaʻau ʻana: ka wili ʻana me ka naikokene wai a i ʻole ka ʻili ʻana o ka ʻiʻo homogenizer
ʻO ka manaʻo o ka Agarose gel: 1% (TAE)
Hoʻouka nui: 2 μl
Hoailona: D15000, TIANGEN
He Hui: he brand kaulana
ʻO ka hopena hoʻokolohua: me ka hoʻohana ʻana iā TGuide S16 me ka reagent 4993548 e unuhi aunoa i ka genomic DNA o nā aʻa palaoa i loaʻa i ka hua maikaʻi a me ka maʻemaʻe kiʻekiʻe, ʻo ia ka mea e like me ka hoʻoulu ʻana a me ka maʻemaʻe o nā mea hoʻokūkū kaulana. Eia kekahi, ʻoi aku ka maikaʻi o ka genomic DNA ma mua o nā huahana hoʻokūkū o nā ʻoihana ʻē aʻe. Ma kēia hoʻokolohua, ma kahi o 15 μg o ka nucleic acid i unuhi ʻia mai 100 mg aʻa palaoa, me OD260/OD280 ma kahi o 1.8~1.9, a me OD260/OD230＞2.0.
