Nā genetics genetics
Loaʻa nā lawelawe lawelawe
Takagi et al.,ʻO ka mea hoʻohana nui, 2013
●Ke nānā neiʻo Bioinmetic Gainmeticicic.hiki i ka manaʻo o keʻano Genetic
●Koho koho koho: e like me keʻano o ka manaʻo o ka manawa divergence a me ka wikiwiki e pili ana i nā loli ma nāʻano likeʻole a me ka pae amino.
●Nā hōʻike loea a me nā moʻolelo paʻi: Ua hōʻiliʻiliʻo BMKGENE i kaʻike nui ma ka heluna a me nā hana he 1000 kiʻekiʻe, a laweʻia ma luna o nāʻano he 1000 kiʻekiʻe
● akamai akamai bioinformatics a me ka pōkole pōkole pōkole: Me kaʻike nui i ka loiloi genomes genomes, e hāʻawi ana i ka hui o BMKGENE BMKGENT i nā loiloi i hoʻopihaʻia me kahi manawa swift turnaround.
● Kākoʻo kākoʻo:Hoʻopili kā mākou kūpaʻaʻana ma mua o ka hoʻopauʻana i kahi papa hana me kahi manawa lawelawe 3-mahina ma hope o ka lawelawe lawelawe. I kēia manawa, hāʻawi aku mākou i nā hana hahai, a me nā manaʻo e hoʻopiʻiʻia i kōkua, a me ke kau e pili ana i nā noi.
Nā mea lawelawe a me nā koi
| ʻAno o ka selencing | Hoʻonohonohoʻia ka heluʻana | Serenencing hoʻolālā | Nā koi likeʻole |
| ʻO ka squencing genome | ≥ 30 mau kānaka, me ≥ 10 mau kānaka mai kēlā me kēia subgroup
| 10X | Ke kūleʻa: ≥ 1 NG / μl Ka huina holoʻokoʻa. 30ng Palenaʻole a iʻole ka degradation a iʻole ka hopena |
| ʻO nā'āpana kiko'ī kiko'ī kiko'ī kiko'ī (slaf) | E hoʻopaʻaʻia: 10X Ka helu o nā hōʻailona: <400 MB: Ua kauohaʻia nā WGS <1GB: 100K Tags 1GB > 2GB: 300K Tags Max 500k Tags | Kaʻikeʻana ≥ 5 NG / μl Huina nui ≥ 80 ng Nanodrop od260 / 280 = 1.6-2.5 Agarose Gel:ʻAʻole a iʻole a iʻole ka palena palena
|
E holo ka lawelawe lawelawe
Hoʻolālā hoʻokolohua
Nā Hoʻounaʻana
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
ʻO ka lawelawe me ka loiloiʻana o ka heluʻana o ka lehulehu lehulehu Hiki i ka lawelawe ke komo pū me ka loiloi maʻamau (e like me ka manawa divergence manawa, gelow wai).
*ʻO nā hopena demo i hōʻikeʻia ma aneʻi mai nā mea āpau mai Genom i paʻiʻia me BMKGENE
Ua loaʻa i nā loiloi o ka loiloi o Phylogenetic lāʻau, ka hoʻolālāʻana o ka lehulehu a me ka PCA e pili ana i nāʻano Genetic.
He lāʻauʻo Phylohagenethic hey i hōʻikeʻia heʻauhau kālā a me nā pilina ho'āʻo i waena o nā 'ōlelo me Genestor.
ʻO PCA AFIMS eʻike i ka hoʻopiliʻana ma waena o nā helu sub-faiculations.
Hōʻike ka hoʻolālāʻana o ka poʻe lehulehu i ka heleʻana o keʻano o keʻano o keʻano o keʻano o keʻano o nā'ōlelo a Allele maʻamau.
Chen, et. al.Pnas, 2020
2.selelectiv
Ua kohoʻia nā mea kōkua e koho i ke kaʻina hana ma kahi o ka pūnaewele kūpono e koho ai a me nā'ōnaehana o nā kikowaena piliʻole a me nā pūnaewele i hoʻololiʻia.
ʻO Genome-ākea kaʻikeʻana i nā wahi koho koho e hoʻopiliʻia e ka heluʻana i nā helu genetic geetic (10 kb).
ʻO nā meaʻokoʻaʻole (π)
Tajaimi's d
Ka hoʻonohonoho kuhikuhiʻana (FST)
Wu, et. al.KA KAILLO MOLLA, 2018
3. E kahe ana
Wu, et. al.KA KAILLO MOLLA, 2018
4.Demographic mōʻaukala
Zhang, et. al.Naʻanoʻona / evolution, 2021
ʻO ka manawa 5.ddivergrence
Zhang, et. al.Naʻanoʻona / evolution, 2021
Eʻimi i nā mea holomua i hoʻomaikaʻiʻia e nā lawelawe a BMKGENE BMKGENE BMKDERE ma o ka hōʻiliʻili o nā paʻi.
ʻO Hassanyar, ak et al. (2023) 'ike' o nā hōʻailona molena snor Moolecular a me nā moho moho i pili i ka srana cerana cerana ma Apis ceranaNā Kūlana Kūlana o Molecular o Molecular, 24 (7). Doi: 10.3390 / IJMS24076238.
CII, J. et al. (2022) 'Accessylelo' o kahi mea hihiu, General Chinese Giamander Giant Giamander e hana i nā manawa o ka mālamaʻana i nā manawaʻO ka noiʻi zoological, 2022, Vol. 43, Kauoha 3,ʻAoʻao: Nāʻaoʻao: 469-480, 43 (3), Pp.. 469-480. Doi: 10.24272 / J.issn.2095-8137.2022.101.
Han, M. et al. )Mau mea i loko o kaʻepekemaʻepekema, 13, p. 882601. Doi: 10.3389 / FPls.20222.882601 / Bibtex.
Wang, j. et al. (2022) nā meaʻike genomic i loko o Longan Evolution mai kahi huiʻo Chromosome-Level a me ka nui o nā hanana GenomHōʻike Huakaʻi Hōʻailona, 9. Doi: 10.1093 / Hr / Uhac021.
