Eukaryonotic MRna Senenecing-Ns
Nā hiʻohiʻona
● hopu iā Poly Mrna ma mua o ka hoʻomākaukauʻana o ka waihona
● E koho i ka koho kuhikuhi TRNA SPNAL TRNA SAME e hiki ai i ka loaʻaʻana o ka loaʻaʻana o kaʻikepili stequing
● Biinformatic kiko'ī o Ge ge gegress a me keʻano transcript
Loaʻa
●ʻO kaʻike nui: Ua hoʻolahaʻia kā mākou hui ma luna o 600,000 mau hua'ōlelo ma bmkgene, nā aniani sampler sampler, nāʻano moʻomeheu e like me nāʻano cell, nāʻano kino kino, a me nā kino kino. Lawe mākou i kahi waiwai o kaʻike i kēlā me kēia papahana a ua hoʻokō maikaʻi ma luna o 100,000 mau papahana MRNA-Seq hana ma nāʻano kiko'ī likeʻole.
●ʻO ka mana kūpono: Ua hoʻokō mākou i nā wahi hoʻokele waiwai ma waena o nā wahi āpau, mai ka papa kūʻai a me nā hale kūʻai hale a me Biinforming. Hoʻomaopopo kēia meticulous i ka laweʻana i ka hāʻawiʻana i nā hopena kiʻekiʻe, e hōʻoiaʻiʻo ana iāʻoe e hilinaʻi i kāu hana.
●KA MANAO KAHAKANA: Ua hoʻohana mākou i nāʻikepili lehulehu e hana i ka hanaʻana i nāʻano likeʻole i hōʻikeʻia (degs) a hana i nā loiloi loiloi Hāʻawi kēia hele i kēia meaʻike i loko o nā'ōnaehana o ke kelepona a me nā molecular i lalo o ka paneʻana i ka transpular.
●Kākoʻo kākoʻo: Ke hoʻonui nei kā mākou hana i waho ma mua o ka hoʻopauʻana i kahi papa hana me kahi 3-mahina ma hope o ka lawelawe lawelawe. I kēia manawa, hāʻawi aku mākou i nā hana hahai, a me nā manaʻo e hoʻopiʻiʻia i kōkua, a me ke kau e pili ana i nā noi.
Nā koi koi a me ka laweʻana
| Puka Hanau | Serenencing hoʻolālā | Ua'ōleloʻia nāʻikepili | Honua mālamalama |
| Poly i kauʻia | Hoʻomālamalama pe150 Dnbseq-t7 | 6 -10 GB | Q30≥85% |
Nā Manaʻo Manaʻo:
Nucletides:
| SCA. (NG / μl) | Ka nui (μg) | ʻO Menu ka hoʻomaʻemaʻe | Huakai | |
| Hale waihona kahua | ≥ 10 | ≥ 0.2 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Palenaʻole aʻaʻohe protein a iʻole dna conceation i hōʻikeʻia ma ka gel. | No nā mea kanu: Rin≥4.0; No nā holoholona: rin≥4.5; 5.0≥28s / 18s. kaupalena a iʻole he kumu kūʻai baseline |
| Waihona Kūlana Kūlana | ≥ 10 | ≥ 0.2 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Palenaʻole aʻaʻohe protein a iʻole dna conceation i hōʻikeʻia ma ka gel. | No nā mea kanu: Rin≥4.0; No nā holoholona: rin≥4.5; 5.0≥28s / 18s. kaupalena a iʻole he kumu kūʻai baseline |
● Nā mea kanu:
All, stem or petal: 450 mg
Lau a iʻole hua: 300 mg
Nā hua: 1.2 g
●Nā holoholona:
Naʻau a iʻole nā'ōpū: 300 mg
Viscera a lolo paha: 240 mg
Muscle: 450 mg
Iwi, lauoho lauoho aʻili paha: 1g
● artoropods:
Nā mea pepeke: 6g
Crustaca: 300 mg
● koko āpau: 1 Tube
● nā cell: 106 manu
Hōʻikeʻia ka hoʻounaʻana
Nā pahu: 2 ML Cetrifuge Tube (Tin Foilʻaʻole i aʻoʻia)
Sample Labelning: Kumupa + Ka Hoʻolau'Awele iā A1, A2, A3; B1, B2, B3 ...
Hoʻouna:
1.ʻO ka maloʻo maloʻo: Pono nā specles i hoʻopaʻaʻia i nāʻeke a kanuʻia ma ka maloʻo maloʻo.
2.ʻO nā papa papa RNas: Hiki ke maloʻoʻo RNA i ka RNA Scabillization Tube (e like me rnastable®) a hoʻounaʻia i ka mahana o ka lumi.
E holo ka lawelawe lawelawe
Hoʻolālā hoʻokolohua
Nā Hoʻounaʻana
RNA extraction
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
Nāʻano bioniformatic
Emunoryotic ʻO MRNA SEQUANCING INGENTISGING PLACKEL
Nāʻano bioniformatic
ØʻO ka mana o nāʻikepili maikaʻi
ØKe kuhikuhiʻana o Genome Algenment
ØKe hoʻohanaʻana i keʻano hoʻolālā
ØKa hōʻike hōʻike
ØʻO nā loiloi hōʻikeʻokoʻa
ØHana hana a me ka hoʻonuiʻana
Ke kuhikuhiʻana o Genome Algenment
ʻO ka hōʻikeʻikeʻikeʻikeʻike
Sample correlation a me ka loiloiʻana i nā'ōlelo hou
I hōʻikeʻia i nā genes (degs)
Hana i kahi annotation o nā degs
Hana hoʻoikaika i nā degs
Eʻimi i nā mea holomua i hoʻomaikaʻiʻia e BMKGENOTOTOTOCTION NGK MRNA MRNA SEQUENING PERSQIENCING PERSQIECING O kahi hōʻiliʻili o nā paʻi.
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Wen, x. et al. (2022) 'The CHRSASATUMMUM Lavandulifium Senme a me ka molena molena i lalo o ka hale waihona puke.
Zhang, yujie et al. (2023) 'kahi mea lawe cascade no ka hoʻonuiʻana i ka hana sonodynamic ma keʻano he colner solner' antophagol stner ', 49,ʻo ia i kēia lā, 49, p. 101798. Doi: 10.1016 / J.nantod.2023.101798.
