ʻO nā hale waihona pukeʻo DNbseq mua
Nā hiʻohiʻona
●Kahua:MgI-dnbseq-t7
●ʻO nā modes:Peemona
●Hoʻololi i nā hale waihona puke maʻi maʻi maʻiMgi:Hoʻomoeʻana i ka squning i nā helu data kiʻekiʻe ma ke kumukūʻai haʻahaʻa.
●Ka mana o nā hale waihona puke ma mua o ka selenencing.
●Sequencing data QC a me ka laweʻana:ʻO ka hāʻawiʻana i ka hōʻike QC a me kaʻikepili i kaʻikepili wikiwiki ma hope o ka nānāʻana ma hope o ka demultiplexing a me kāna kiʻina.
Loaʻa
●ʻO kaʻoiaʻiʻo o nā lawelawe sequence:Hiki i ka mea kūʻai ke koho i keʻano o ke ala a iʻole ka nui o nāʻike.
●Ka heluʻikepili kiʻekiʻe:1500 GB / Lanee
●KAHANA O KA LOHE ANA O NUI QC:Me nā metricits maikaʻi,ʻo kaʻikeʻikepili a me ka hana holoʻokoʻa o ka papahana seequence.
●HE KAI LOA NUI:me ka huli pōkole-a puni ka manawa.
●ʻO ka mana kūpono: Ua hoʻokō mākou i nā koi QC e hōʻoia i ka hōʻoiaʻana i ka hāʻawiʻana i nā hopena kiʻekiʻe.
Nā koi koi
| Ka helu helu (x) | Ka noʻonoʻo (qpcr / nm) | Volume | |
| 'Āpanaʻaoʻao
| X ≤ 10 gb | ≥ 1nm | ≥ 25 μl |
| 10 GB <x ≤ 50 GB | ≥ 2 nm | ≥ 25 μl | |
| 50 GB <x ≤ 100 GB | ≥ 3 nm | ≥ 25 μl | |
| X> 100 GB | ≥ 4 nm | ||
| Mea kū hoʻokahi | Ma ke ala | ≥ 1.5 nm / ka waihona puke | ≥ 25 μl / hale waihona puke |
Ma kahi o ka loaʻaʻana a me ka nui o ka nui, e koiʻia kahiʻano kūpono kūpono.
Kahakaha: Lane Sequening o nā hale waihona liʻiliʻi e pono aiʻo PHIX Spike
Manaʻo mākou e waiho i nā hale waihona puke mua ma mua o nā hiʻohiʻona. Inā makemakeʻoe iā BMKGENE e hana i ka mea hana waihona puke, eʻoluʻolu e pili i ka
Nā pono waihona waihona no ka mahele moku.
Ka waihona waihona (Peak Map)
Ponoʻo Main Peak i loko o 300-450 BP.
Pono nā hale waihona puke i hoʻokahi pākuʻi nui,ʻaʻohe adapter e kū ana aʻaʻohe dimers mua.
Haʻawina lawelawe
Hōʻike QC waihona puke
Hāʻawiʻia kahi hōʻike ma keʻano o ka waihona puke ma mua o ka seleving, e nānā ana i ka nui o ka waihona kālā, a me ka hoʻopunipuni.
Seequing qc hōʻike
Papa 1. Nā helu helu ma nā helu sequencing.
| Kōkuhi ID | BMKID | Heluhelu mai nā ana | ʻO nāʻikepili RAW (bp) | E hoʻomaʻemaʻeʻia (%) | Q20 (%) | Q30 (%) | GC (%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | Bmk_02 | 14,717,,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Kiʻi 1.ʻO ka hoʻokaʻawaleʻana o ka maikaʻi ma ka heluheluʻana i kēlā me kēia hoʻohālike
Kiʻi 2. Kūʻai Kūlana Kūlana
Kiʻi 3.ʻO ka hāʻawiʻana i nā mea heluhelu heluhelu i nāʻikepili seequing
