Kākāika Hāneku - Illumina
Nā hiʻohiʻona
● Rrna i hanaʻia e ka hoʻomakaʻana o ka papa inoa o ka hale kūʻai
●ʻO ka hanaʻo Biinformatic Workfy e hiki ai i ka wānana secrorna a me ka hōʻike hōʻike
Loaʻa nā lawelawe lawelawe
●ʻO nā hale waihona puke hou loa:Hoʻohana mākou i ka RRNA i kahi o ka laina RNANAR RNA i kā mākou hoʻomākaukau muaʻana, e hōʻoia i kaʻikeʻana o ka SEQUNY i kēia mau kikowaena
●ʻO ke koho koho o ka hoʻokūkū hoʻokūkū RNATogenous RNA (CERNA) Networks: Hāʻawi i nā mea nānā hohonu i loko o nā mīkini regulators
●ʻO kaʻike nui: Me kahi kiʻi kiʻi kiʻi o ka hoʻoiliʻana ma luna o 20,000 mau kumuhana ma BMKGEN,'Āpana Kūʻai, e lawe ana kā mākou hui i kahi waiwai o kēlā me kēia papahana.
●ʻO ka mana kūpono: Ua hoʻokō mākou i nā wahi hoʻokele waiwai ma waena o nā wahi āpau, mai ka papa kūʻai a me nā hale kūʻai hale a me Biinforming. Ke hōʻoia nei kēia meticulous e hōʻoia i ka hāʻawiʻana i nā hopena kiʻekiʻe kiʻekiʻe.
● Kākoʻo kākoʻo: Ke hoʻonui nei kā mākou hana i waho ma mua o ka hoʻopauʻana i kahi papa hana me kahi 3-mahina ma hope o ka lawelawe lawelawe. I kēia manawa, hāʻawi aku mākou i nā hana hahai, a me nā manaʻo e hoʻopiʻiʻia i kōkua, a me ke kau e pili ana i nā noi.
Nā koi koi a me ka laweʻana
| Puka Hanau | Kahua | ʻO kaʻikepili iʻikeʻia | Data qc |
| Ua loaʻa iā Rrna i kahi waihona waihona | Hoʻomālamalama pe150 | 16-20 gb | Q30≥85% |
Nā Manaʻo Manaʻo:
Nucletides:
| SCA. (NG / μl) | Ka nui (μg) | ʻO Menu ka hoʻomaʻemaʻe | Huakai |
| ≥ 80 | ≥ 0.8 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Palenaʻole aʻaʻohe protein a iʻole dna conceation i hōʻikeʻia ma ka gel. | Rin≥6.0; 5.0≥28s / 18s. kaupalena a iʻole he kumu kūʻai baseline |
● Nā mea kanu:
All, stem or petal: 450 mg
Lau a iʻole hua: 300 mg
Nā hua: 1.2 g
● nā holoholona:
Puʻuwai a iʻole intestine: 450 mg
Viscera a lolo paha: 240 mg
Muscle: 600 mg
Iwi, lauoho lauoho a iʻole kaʻili: 1.5g
● artoropods:
Inikini: 9g
Custaca: 450 mg
● He koko āpau:2 paila
● cells: 106 manu
● semas a plasma: 6 ml
Hōʻikeʻia ka hoʻounaʻana
Nā pahu: 2 ML Cetrifuge Tube (Tin Foilʻaʻole i aʻoʻia)
Sample Labelning: Kumupa + Ka Hoʻolau'Awele iā A1, A2, A3; B1, B2, B3.
Hoʻouna:
1.ʻO ka maloʻo maloʻo: Pono nā specles i hoʻopaʻaʻia i nāʻeke a kanuʻia ma ka maloʻo maloʻo.
2. E hiki ke maloʻoʻo Rnastable: Hiki ke maloʻoʻo RNA i ka RNA Stibalization Tube (e like me, rnastable®) a hoʻounaʻia ma ka mahana o ka lumi.
E holo ka lawelawe lawelawe
Hoʻolālā hoʻokolohua
Nā Hoʻounaʻana
RNA extraction
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
Nāʻano bioniformatic
Kuku'āinaʻo Cirstrre: Chromosalm ka hoʻoili
Hoʻohuiʻia nā cirgnas
I hōʻikeʻia i nā cirrrnas - hierarchical clustering
Hana hoʻoikaika i nā kikowaena o nā gene
Eʻimi i ka hana noiʻi noiʻi e hoʻomaʻamaʻaʻia e BMKGEE 'SCIRECNAE SEQUENCING e lawelawe ma kahi hōʻiliʻili o nā paʻi.
Wang, x. et al. (2021) 'Cpsf4 E hoʻoponopono i ka papa hana a me MIMORNA ma Mediady ma kahi o Hepatocener Carcinomalar Doi: 10.1038 / S41388-021-01867-6.
Xia, k. et al. (2023) 'X O OO-pane i hōʻikeʻia i ka hana o ka rna133 ma ka hōʻikeʻana o ka maʻi o ka laiki Doi: 10.1186 / S42483-023-00188-88 / helu / 6.
Y, h. et al. (2023) 'cpsf3 e hoʻololi i ke kaulike o ka circular a me ka laina i nā kope ma Hepatocellur Doi: 10.21203 / Rs.3.S-2418311 / V1.
Zhang, y. et al. (2023)ʻO ka loiloi loiloi o nā circnas i mua o carrhotic cardiotypathy ma mua a ma hope o keʻano o keʻano o ke olaʻana, 114, p. 109495. Doi: 10.1016 / J.intimp.202222222.109495.
