Chromatin immuncrecipitation serenencing sreequing (chip-seq)
Loaʻa nā lawelawe lawelawe
●ʻO ka loiloi bioinnextic kiʻekiʻe a me ka hōʻoiaʻana i ka annotation.Hoʻohana mākou i nāʻikepili lehulehu e hana i ka hanaʻana i nā hua'ōlelo e pili ana i nā inoa o ka profile-dna binding, e lawe ana i nā kaʻina hana ma lalo o ka huiʻana.
●Kākoʻo kākoʻo:Hoʻopili kā mākou kūpaʻaʻana ma mua o ka hoʻopauʻana i kahi papa hana me kahi manawa lawelawe 3-mahina ma hope o ka lawelawe lawelawe. I kēia manawa, hāʻawi aku mākou i nā hana hahai, a me nā manaʻo e hoʻopiʻiʻia i kōkua, a me ke kau e pili ana i nā noi.
●Kaʻike nui:Me kahi papa mele e hoʻokō pono ai i nā papahana chip-seq e loaʻa ana, e lawe mai kā mākou hui ma luna o kahi nui o ka papa. ʻO kā mākou hui loiloi koʻikoʻi, ua hui pū me nāʻike kiko'ī a me nā kākoʻo o nā mea kūʻai aku, e hōʻoia i ka kūleʻa o kāu mau papahana.
● Hoʻonani maikaʻi loa: Ua hoʻokō mākou i nā wahi hoʻokele waiwai ma waena o nā wahi āpau, mai ka papa kūʻai a me nā hale kūʻai hale a me Biinforming. Ke hōʻoia nei kēia meticulous e hōʻoia i ka hāʻawiʻana i nā hopena kiʻekiʻe kiʻekiʻe.
Nāʻikepili lawelawe
| Puka Hanau | Serenencing hoʻolālā | ʻO kaʻikeʻikepili i hōʻikeʻia | Honua mālamalama |
| Wehe i ka dna ma hope o ka imguncrecipition | Hoʻomālamalama pe150 | 10GB | Q30≥85% Cisulfite hoʻololi> 99% Mspi cution effing effiency> 95% |
Nā koi koi
Ka nui nui: ≥10 NG
ʻO ka hoʻokaʻawaleʻana i ka nui o ka nui: 100-750 bps
E holo ka lawelawe lawelawe
Nā Hoʻounaʻana
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
Komo i ka loiloi ma lalo nei:
● Ke Kahu Pūnaewele Pūnaewele
● Ke kāheaʻana i ke kauʻana ma luna o ka mapping e kuhikuhi i ka genome
● annotation o nā genes pili
●ʻO keʻano hoʻohālike
● Activeial Peak Opecty a me ka anNontation
ʻO ka loiloi o ka enrichment ma kahi kokoke i nā pūnaewele hoʻomaka (TSSs)
ʻO Genome-ākea ākea o nā pīkī chip
ʻO ka papa inoa o nā wahi kiʻekiʻe
Hana hoʻoikaika e pili ana i nā genes e pili ana i nā genes (Kegg)
