De novo bacterial genome
Hāʻawi mākou i kahi lawelawe Genome Genome Genome Genome, e hōʻoiaʻiʻo ana i 0 nā gaps. Hiki i kēia ma ka hoʻopiliʻana i nāʻenehana lōʻihi e heluhelu ai, e like me ka inoaʻo NanoPore a me Pacbio no ka'ōleloʻana a me ka hoʻopunipuni Hāʻawi ka lawelaweʻoihana lawelawe i ka pā kula piha mai ka hālāwaiʻana, e hōʻike ana i ka hoʻonāukiuki, a hoʻomaikaʻiʻo Biyhionitytics i nā pahuhopu noiʻi kūikawā. Mālama kēia lawelawe i ka hoʻomohalaʻana i nā'ōlelo kiko'ī no nā genomes no nā haʻawina genetic a me genomic. Eia kekahi, hana ia i ke kumu no nā noi e like me ka hoʻolālāʻana, a me kaʻenehana loea genemic
Nā hiʻohiʻona lawelawe
● Me nā kohoʻelua e koho ai i ke kohoʻana mai ke kiʻekiʻe o ke kiʻekiʻe o Genome.
● Ke kohoʻana i nā koho Genome Genome:
● Hoʻopiha 0 GAP GENOME.
●ʻO ka lōʻihi-heluhelu i ka huakaʻi ma Nanopore Superthion 48 a iʻole pacbio redidio no Genome.
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Loaʻa nā lawelawe lawelawe
●Zero-gap genome hōʻoiaʻia: Aia kēia ma muli o ka hoʻohuiʻana o ka maʻi maʻi maʻi me ka lōʻihi o ka heluheluʻana (nanopore a iʻole ka pacbio).
●ʻO ka piha o bioinformatics piha:Hoʻokomoʻia kēia me keʻano o keʻano genome a me ka wānana o nā mea he nui he nui, hana hana i kaʻano o nā corcosication genome
●ʻO kaʻike nui: Me nā 20,000 mau genmes genomes e'ākoakoa ana,ʻo BMKgene e lawe mai ana i kahi hui o kaʻike, a me ke kākoʻoʻana o ka mea kūʻai aku.
●Kākoʻo kākoʻo:Hoʻopili kā mākou kūpaʻaʻana ma mua o ka hoʻopauʻana i kahi papa hana me kahi manawa lawelawe 3-mahina ma hope o ka lawelawe lawelawe. I kēia manawa, hāʻawi aku mākou i nā hana hahai, a me nā manaʻo e hoʻopiʻiʻia i kōkua, a me ke kau e pili ana i nā noi.
●Loaʻa i nā hoʻolālā squencing e loaʻa:No nā pahuhopu noiʻi likeʻole a me nā koi o nā pono o Genome Fillme.
Nāʻikepili lawelawe
| Hoʻokaumi | Serenencing hoʻolālā |
| Draft genome | ʻO Ihemina Pe150 100x |
| 0 Gpe Genome | Nanoopoore 100x + Hana pe150 100x Or Pacbio Hifi 30x +'Ollumina Pe150 100x (koho) |
Nā Manaʻo Manaʻo:
| Ka Manaʻo (NG / μl) | Ka nui nui (μg) | Nui (μl) | Od260 / 280 | Od260 / 230 | |
| PACBio | ≥20 | ≥1.2 | ≥20 | 1.7-2.2 | ≥1.0 |
| Nana'Ooke | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| ʻO Malomina | ≥1 | ≥0.06 | ≥20 | - | - |
· BACTERIA: ≥3.5x1010 manu
E holo ka lawelawe lawelawe
Nā Hoʻounaʻana
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
Komo i ka loiloi ma lalo nei:
● Ke nānāʻana i ka mana o kaʻikepili
●'Ōlelo Genome
●ʻO ka hoʻohālikelikeʻana o Genome Commonentis: wānana o CD a me nā mea he nui
● Hoʻomoe i ka hanaʻana me nāʻikepili he nui (hele, kegg, etc) a me nā waihona waiwai (kāleka, vfdb, etc)
● Nā Kūlana Genome
Hāʻawi mākou i ka genome i kahi kūlana wikiwiki a hiki i keʻano o ka faile a me ka fenome annote annote file (gff).
Gene Ancodation - Hele
ʻO Gene Annotation - Cazy Carbhydrates
ʻO Genome Visuarization - Circos plot
Eʻimi i ka holomua o nā mea holomua e BMKGENE GENOME SMATERSEMS OMSEMS OMSET ma kahi hōʻiliʻili o nā paʻi.
DI, W. et al. (2023) 'ike' o nā bacterodes i ka f18-22 e like me ka mea palekana a me ka mālamaʻana i ka collit collatiNā Kūlana Kūlana o Molecular o Molecular, 24) 19), p. 14669. Doi: 10.3390 / IJMS241914669 / S1.
Kang, "Ea et al. (2021) 'multidrug-resistitug-resistant mirabilis e lawe ana i ka blaoxa-1 a me blandom-1 mai ke ola olakino.Integrative Zoology, 16 (6), PP. 798-809. Doi: 10.1111 / 1749-4877.12510.
Wang, tt et al. (2017)Nūpepa o biotechnology, 260, pp. 38-41. Doi: 10.1016 / J.JBiotec.2017.09.001.
Wang, x. et al. (2021) '' o-replican resmids of klebsids of Klebiellal Mediate idiate e wehe ana i nā genes antimicrobial genes ',Mau alo ma ka microbiology, 12, p. 754931. Doi: 10.3389 / FMicb.2021.754931 / bibtex.
