16s / 18s / kona mau amplidon seequence-NGS
Nā hiʻohiʻona lawelawe
● Nā Kūlana Kūlana:ʻO Allimuina Novaseq.
●ʻO ka hoʻohuiʻana o nā'āpana pōkole o 16s, 18s a me kāna, i waena o nā mea hou aʻe.
● Nā koho hoʻololi o Amplican.
● Loaʻa i nā hanana papahana ma mua me nā pahuhopu he nui.
Loaʻa nā lawelawe lawelawe
●E hoʻokaʻawale-manuahiʻole:Ka hōʻikeʻana o ka rapid o ka microbal e pili ana i nā hiʻohiʻona o ka honua.
●NA HUI: Ma nā'āpana haʻahaʻa haʻahaʻa ma nā hiʻohiʻona o nā kaiāulu.
●Pili loa: Nā haʻawina kaiāuluʻo Duet Selebial.
●ʻO ka nānāʻana o Bioinia Companintisticictic:ʻO ka uku QIME2 pākuʻi (ʻike nui loa i loko o ka ecology microbial) me nā mea kiko'ī e pili ana i ka waihona pūnaewele, Otuv.
●ʻO kaʻike nui: Me 150 mau tausani hana amplicon seaquencing i hana i kēlā me kēia makahiki, lawe maiʻo BMKgene i kahi kūlana kiʻekiʻe, a me ke kākoʻo maikaʻi loa.
Nāʻikepili lawelawe
| Puka Hanau | Serenencing hoʻolālā | Ua'ōleloʻia nāʻikepili |
| Amplion | ʻO Ifluina Pe250 | 50/100 / 300k Tags (heluhelu i nā pālua) |
Nā koi lawelawe
| Ka Manaʻo (NG / μl) | Ka nui nui (NG) | Nui (μl) |
| ≥1 | ≥200 | ≥20 |
● lepo / sludge: 1-2g
● Nā 'ikepili Instest. holoholona: 0.5-2g
● Nā Kūlana Instestinal: 0.1-0.25G
● Nā Hana Kūʻai (hoʻonuiʻiaʻo SEDITES): 0.1-0.5g
● FUBETATION BROTHITED SEDITED SEDIMENT): 0.1-0.5g
● nā mea aloha (nā holoholona nui): 0.5-2g
● nā mea aloha (kiʻi): 3-5Grains
● pulmonary alveolar livage flud: pepa pepa
● Swaginal Swab: 5-6 Swabs
● E kāwili i kaʻili / genital swab / sheava / orral stisue / pharyngeal swab / rectal swab: 2-3 swabs
● Nā Mīkini Micororanisms: Nā pepa kānana
● Kau wai / nor / biofilm: pepa pepa
● E hoʻohuli: 1-2G
● Opelika Deal: 0.5-1g
E holo ka lawelawe lawelawe
Nā Hoʻounaʻana
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
Komo i ka loiloi ma lalo nei:
- ʻO ka mana o nāʻikepili maikaʻi
- ʻO Otu clustering / de-wire (asv)
- Otu anvotation
- Hōʻikeʻike Alpha Mesversies: Nā helu helu he nui,ʻo Shanon, me Simson, Simpson a me Ace.
- Beta i ka hoʻohālikelikeʻana
- Ka loiloi hui waena
- ʻO ka loiloi Correlation: ma waena o nā mea kūlohelohe a me ka nohoʻana
- 16s hana hana
Kānana o ka hoʻolahaʻana o kaʻauhauʻauhau
ʻO ka nui o nā palapala uila
Alpha Mesversity: Rarefction Curve
ʻO Beta i ka nānāʻana i ka hoʻohuiʻana: NMDS
ʻO ka loiloi Intergroup: Lefse biomarker i loaʻa
Eʻimi i nā mea holomua i hoʻomaikaʻiʻia e BMKLINON GEQILING SEQUNING FEQUENCING me nā maʻi maʻi ma o kahi hōʻiliʻili o nā paʻi.
Dong, c. et al. (2022) Lā Kūke,ʻo Coririota, a me ka hanaʻana o ke anaonaʻo Rhizerota malomakes, 13. Dai: 8553 0 piha.
Li, y. et al. (2023) 'Synthetic Bacterial Consortia Modentia no ka mālamaʻana i ka Vaginalis Vaginasis Vaginalis-indiecel, 11 (1), PP. Doi: 10.1186 / S4018-023-01497-Y
Yeng, j., fe, y. a li li lie o ka lani 1-20. Doi: 10.1186 / S407793-0222-00399-099-0 / kiʻi / 8.
Yin, s. et et al. (2022) '' ōlelo hānai i ka nui o nā genel genel e pili ana i kaʻenehanaʻo nitrogen 127678. Doi: 10.1016 / J.Borort.20222228678.
