Gabaɗaya Tsarin Rubutu - Illumina
Siffofin
● Laburare biyu don jera cikakken rubutun: Ragewar rRNA tare da shirye-shiryen ɗakin karatu na PE150 da zaɓin girman da ke biye da shirye-shiryen ɗakin karatu na SE50.
● Cikakken nazarin bioinformatics na mRNA, lncRNA, circRNA, da miRNA a cikin rahotannin bioinformatics daban-daban.
● Binciken haɗin gwiwa na duk maganganun RNA a cikin rahoton haɗin gwiwa, gami da nazarin cibiyoyin sadarwa na ceRNA.
Amfanin Sabis
●Bincike mai zurfi na hanyoyin sadarwa na tsari: Ana yin nazarin hanyar sadarwar ceRNA ta hanyar haɗin haɗin gwiwar mRNA, lncRNA, circRNA, da miRNA kuma ta hanyar ingantaccen aiki na bioinformatic.
●Cikakken Bayani: Muna amfani da bayanai da yawa don yin bayanin aikin Halittun Halittu (DEGs) da kuma yin ƙididdigar haɓaka daidai, samar da fahimta cikin tsarin salon salula da tsarin ƙwayoyin cuta waɗanda ke ƙarƙashin amsawar kwafin.
●Ƙwararren Ƙwararru: Tare da rikodin waƙa na nasarar rufe sama da 2100 gabaɗayan ayyukan kwafi a cikin yankuna daban-daban na bincike, ƙungiyarmu tana kawo ƙwararrun ƙwarewa ga kowane aiki.
●Tsananin Kula da Inganci: Muna aiwatar da mahimman abubuwan sarrafawa a duk matakai, daga samfurin da shirye-shiryen ɗakin karatu zuwa jerin abubuwa da bioinformatics. Wannan saka idanu mai kyau yana tabbatar da isar da sakamako mai inganci akai-akai.
●Tallafin Bayan Talla: Alƙawarinmu ya wuce bayan kammala aikin tare da lokacin sabis na watanni 3 bayan-sayar. A wannan lokacin, muna ba da bibiyar aikin, taimako na warware matsala, da kuma zaman Q&A don magance duk wata tambaya da ta shafi sakamakon.
Samfuran Bukatun da Bayarwa
| Laburare | Dabarun jeri | An ba da shawarar bayanai | Kula da inganci |
| rRNA ya ƙare | Farashin PE150 | 16 gb | Q30≥85% |
| Girman da aka zaɓa | Illumina SE50 | 10-20M karatu |
Samfuran Bukatun:
Nucleotides:
| Conc.(ng/μl) | Adadin (μg) | Tsafta | Mutunci |
| ≥ 80 | ≥ 1.6 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Iyakance ko babu furotin ko gurɓataccen DNA da aka nuna akan gel. | RIN≥6.0 5.0≥28S/18S≥1.0; iyakance ko babu girma daga tushe |
Isar da Samfurin Nasiha
Kwantena: 2 ml bututu centrifuge (Ba a ba da shawarar foil tin ba)
Alamar samfur: Ƙungiya+ kwafi misali A1, A2, A3; B1, B2, B3.
Kawo:
1. Busasshen ƙanƙara: Ana buƙatar samfurori a cikin jaka kuma a binne su a bushe-kankara.
2. RNAstable tubes: Ana iya bushe samfuran RNA a cikin bututun daidaitawa na RNA (misali RNAstable®) kuma a tura su a zafin jiki.
Gudun Aikin Sabis
Gwajin ƙira
Samfurin bayarwa
RNA cirewa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
Bioinformatics
Bayanin magana na RNA
Ƙwayoyin Halittu Daban-daban
ceRNA analysis
MiRNAs da aka bayyana daban-daban da RNA masu alaƙa
Bincika ci gaban binciken da BMKGene ya sauƙaƙe ta sabis ɗin jeri na kwafi ta hanyar tarin wallafe-wallafe.
Dai, Y. (2022) 'Mahimman bayanan bayanan mRNAs, lncRNAs da miRNAs a cikin cutar Kashin-Beck da aka gano ta hanyar RNA-sequencing', Molecular Omics, 18(2), shafi 154-166. doi: 10.1039/D1MO00370D.
Liu, N. nan et al. (2022) 'Cikakken bayanan kwafi na nazarin juriya na sanyi na Apis cerana a cikin tsaunin Changbai a lokacin wuce gona da iri.', Gene, 830, shafi 146503-146503. doi: 10.1016/J.GENE.2022.146503.
Wang, XJ et al. (2022) 'Multi-Omics Integration-Bassed Pribased of Gasar Ƙarfin Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru', Ƙwararrun Ƙwararru a Oncology, 12, p. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xu, P. et al. (2022) 'Harfafa bincike na lncRNA/circRNA-miRNA-mRNA bayanan bayanan martaba yana bayyana sabbin ra'ayoyi game da hanyoyin da za a iya amfani da su don mayar da martani ga tushen-ƙulli a cikin gyada', BMC Genomics, 23(1), shafi 1-12. doi: 10.1186/S12864-022-08470-3/FIGURES/7.
Yan, Z. et al. (2022) 'Tsarin RNA gabaɗaya yana ba da haske kan hanyoyin kwayoyin da ke da alaƙa da kiyaye ingancin girbi a cikin broccoli ta hanyar hasken haske na LED', Biology Biology and Technology, 188, p. 111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.
