Gabaɗaya jerin kwayoyin halitta bisulfite (WGBS)
Duka Genome Bisulfite Sequencing (WGBS) yana tsaye a matsayin tsarin daidaitaccen zinari don zurfin bincike na DNA methylation, musamman matsayi na biyar a cikin cytosine (5-mC), mai mahimmanci mai daidaita maganganun kwayoyin halitta da ayyukan salula. Ka'idar da ke ƙarƙashin WGBS ta ƙunshi jiyya na bisulfite, haifar da jujjuyawar cytosines marasa daidaituwa zuwa uracil (C zuwa U), yayin barin cytosin methylated ba canzawa. Wannan dabarar tana ba da ƙuduri mai tushe guda ɗaya, yana bawa masu bincike damar bincikar methylome gabaɗaya kuma su gano tsarin methylation mara kyau da ke da alaƙa da yanayi daban-daban, musamman kansa. Ta hanyar amfani da WGBS, masana kimiyya za su iya samun haske mara misaltuwa cikin yanayin shimfidar wurare masu faɗi na methylation, suna ba da cikakkiyar fahimta game da hanyoyin epigenetic waɗanda ke haifar da matakai da cututtuka iri-iri.
Siffofin Sabis
● Yana buƙatar nau'in kwayar halitta.
● Ana ƙara DNA na Lambda don saka idanu da ingantaccen jujjuyawar bisulfite.
● Sequencing akan Illumina NovaSeq.
Amfanin Sabis
●Matsayin Zinariya don Binciken Methylation DNA: Wannan balagagge methylation fasahar sarrafa canji yana da babban daidaito da kuma mai kyau reproducibility.
●Faɗin Rufewa da Ƙimar Tushe Guda:gano wuraren methylation a matakin genome-fadi.
●Cikakken Dandali:samar da kyakkyawan sabis na tsayawa ɗaya daga sarrafa samfurin, ginin ɗakin karatu, jeri zuwa nazarin bioinformatics.
●Ƙwararren Ƙwararru: tare da jerin ayyukan WGBS da aka samu nasarar kammala su a cikin nau'i-nau'i daban-daban, BMKGENE ya kawo fiye da shekaru goma na gwaninta, ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararru .
●Yiwuwar Haɗawa tare da Binciken Rubutu: ba da izinin haɗaɗɗen bincike na WGBS tare da sauran bayanan omics kamar RNA-seq.
Ƙayyadaddun Misali
| Laburare | Dabarun Jeri | Fitowar bayanai da aka ba da shawarar | Kula da inganci |
| Bisulfite da aka yi | Farashin PE150 | 30x zurfin | Q30 ≥ 85% Canjin Bisulfite> 99% |
Samfuran Bukatun
| Hankali (ng/µL) | Jimlar adadin (µg) | Ƙarin buƙatun | |
| Genomic DNA | ≥ 5 | ≥ 400 ng | Ƙayyadaddun lalacewa ko gurɓatawa |
Gudun Aikin Sabis
Samfurin bayarwa
Cire DNA
Gina ɗakin karatu
Jeri
Binciken bayanai
Isar da bayanai
Ya haɗa da bincike mai zuwa:
● Raw sequencing ingancin kula;
● Yin taswira zuwa ga kwayoyin halitta;
● Gano sansanonin methylated 5mC;
● Nazarin rarraba methylation da annotation;
● Binciken Yankunan Methylated Daban-daban (DMRs);
● Bayanin aiki na kwayoyin halitta masu alaƙa da DMRs.
5mC ganowar methylation: nau'ikan wuraren methylated
Taswirar methylation. 5mC methylation genome-fadi rarraba
Bayanin yankuna masu methylated sosai
Yankunan Methylated daban-daban: kwayoyin halitta masu alaƙa
Yankunan Methylated daban-daban: bayanin kwayoyin halitta masu alaƙa (Gene Ontology)
Bincika ci gaban bincike ta hanyar BMKGene gabaɗayan hidimomin bisulfite na genome ta hanyar tarin wallafe-wallafe.
Fan, Y. et al. (2020) 'Bincike bayanan bayanan DNA methylation yayin haɓakar tsokar tumaki ta hanyar amfani da tsarin bisulfite gabaɗaya',BMC Genomics, 21 (1), shafi na 1–15. doi: 10.1186/S12864-020-6751-5.
Zhao, X. et al. (2022) 'Novel deoxyribonucleic acid methylation perturbations a cikin ma'aikatan da aka fallasa ga vinyl chloride',Toxicology da Lafiyar Masana'antu, 38 (7), shafi na 377-388. doi: 10.1177/07482337221098600
Zuo, J. et al. (2020) 'Dangantaka tsakanin genome methylation, matakan RNAs marasa coding, mRNAs da metabolites a cikin ripening 'ya'yan tumatir',Jaridar Shuka, 103 (3), shafi na 980-994. doi: 10.1111/TPJ.14778.
