Karamin RNA Sequecting-Illumina
Fasas
● Shirye-shiryen laburare ya haɗa da matakan zaɓi
● Bioin normormatic nazarin da ke tsakiya a kusa da Merna Hasashen da makasudinsu
Fa'idodi na sabis
●Cikakken bincike na bincike:Sanya shaidar tantance na biyu da aka sani da kuma tantance maƙasudin gungun Mirnas, da kuma daidaitawa da bayanan aiki mai amfani da yawa (Kogg, Go)
●Tsauri mai inganci: Muna aiwatar da wuraren sarrafawa a duk faɗin duk matakai, daga samfurin da ɗakin karatu zuwa sequincing da kuma yin amfani da biinhormatics. Wannan sauke mai ban sha'awa yana tabbatar da isar da sakamako mai inganci.
●Tallafin Bayanan Wasanni: Jagorarmu ta shimfida bayan kammala aikin tare da lokacin 3 bayan sayar da sabis. A wannan lokacin, muna bayar da fa'idar bi, don taimako na matsala, da kuma zaman don magance duk wasu tambayoyin da suka shafi sakamakon.
●Mai yawan ƙwarewa: Tare da rikodin rikodin rikodin ya dawo kan ayyukan da yawa na Srna da yawa a cikin nau'ikan bincike daban-daban, ƙungiyarmu tana kawo ɗanɗan da yawa ga kowane aiki.
Bukatar samfuri da isarwa
| Leburare | Tukuba | Bayanan da aka ba da shawarar | Qc |
| Girman zaba | Illumina SE50 | 10m-20m karanta | Q30≥85% |
Bukatun Samfura:
Nucheotides:
| Conc. (NG / μL) | Adadin (μg) | M | Kirki |
| ≥ 80 | ≥ 0.8 | OD260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Iyakantacce ko babu furotin ko gurbataccen DNA wanda aka nuna akan gel. | Rinai6.0; 5.0≥28s / 18S≥1.0; iyakantacce ko babu wani yanki |
● tsire-tsire:
Tushen, kara ko petal: 450 mg
Ganye ko iri: 300 mg
'Ya'yan itace: 1.2 g
● dabba:
Zuciya ko hanji: 450 mg
Viscera ko kwakwalwa: 240 mg
Tsoka: 600 mg
Kasusuwa, gashi ko fata: 1.5g
● arthropods:
Kwari: 9G
Crustacea: 450 MG
Laifi gaba daya: 2 Tambaye
Kwayoyin sel: 106 sel
● Serum da plasma:6 ml
Bayar da Shawara
Akwati: 2 ml centrifuge bututu (tin capil ba da shawarar)
Allalan Samfura: Group + ya dace da misali a1, A2, A3; B1, B2, B3.
Jirgin ruwa:
1. Bushe-kankara: samfures bukatar a cushe a cikin jaka da binne a bushe-kankara.
2. Tumbunan rniastable: Ana iya bushewa sasƙarin RNA a cikin bututun RNA, og rnastable®) kuma an shigo cikin zazzabi a ɗakin.
Aikin aikin sabis
Tsarin gwaji
Samfura
Hakar RNA
Kungiyar Kwadadi
Kazalala
Bincike na bayanai
Bayan Siyarwa
Bioinformatics
Ikon ingancin bayanai
Classigfication ● Srna
Al'umma ga ainihin ambaton
● Shaida da aka sani da kuma ishara mirgina
Fiasearin bayani na Merna
● ANAMIN NA FARKO
Shaida Mirna: Tsarin da zurfi
Bambancin magana ta Mirna - Heciyadical
Bayanin aiki na manufa na bambanci na banbancin Mirnas
Bincika ayyukan bincike na bincike wanda Bmkgene 'Srna ta sauƙaƙe ta hanyar samar da tarin abubuwa ta hanyar tarin littattafan.
Chen, H. et al. (2023) 'Idan akwai cututtukan hoto na hoto na saponin biosynthesis da panyhynthessg', tsire-tsire na kwakwalwa da nazarin halittu, 203, p. 108038. Doi: 10.1016 / J.playz.2023.108038.
Li, H. et al. (2023) 'Itace FY Domain-mai dauke da kayan adon' yan kasuwa kyauta tare da abubuwan da aka gyara microprocessor don tsage ', rahotannin Embo, 24 (1). Doi: 10.15522 / Effr.0225037 / evel0255035503550355037 -p.
Yu, J. et al. (2023) 'MiciSna Ame-Bantam-3P tana sarrafa ci gaban dan Uvalmal ci gaban ci gaban farko-kamar (MEGF8) a cikin zuma na episled, mujallar APIS Mellifera, APIS Mellifera', Melis Mellivivera, 24 (6), p . 5726. Doi: 10.3390 / ijms24065726 / s1.
Zhang, M. et al. (2018) 'Haɗin bincike na Mirna da kwayoyin halitta suna da alaƙa da ingancin nama wanda GGA-Mir-140-33, PP. 2421-2433. doi: 10.1159 / 000489649.
