Exclusive Agency for Korea

BmkCloud Shiga ciki

Kaya

Single- Nuclei RNA Sequincing

Ci gaban kwayar halitta da dabarun gini na al'ada, tare da manyan kayan aiki, ya yi juyin martabar gwaji a matakin sel. Wannan ci gaba yana ba da damar zurfi da ƙarin bincike na rikitarwa da yawan ƙwayoyin cuta, shawo kan iyakokin Gene da kuma adana halaye na gaskiya a cikin waɗannan mutanen. Duk da yake yayin da aka ba da wata sequincing (CNNA-SeQ SeQ) ya sami fa'ida a cikin wasu kyallen takarda inda halittar sutturar sel guda ɗaya ke tabbatar da wahala kuma yana buƙatar sabbin abubuwa. A Bmkgene, mun magance wannan mahaukacin ne ta hanyar bayar da kayan kwalliya na RNA guda biyu (seqeus Seq) ta amfani da fasahar Fasaha ta -x 10x. Wannan hanyar fadada bakan gizo-samawu a cikin bincike na bincike a matakin sel guda ɗaya.

Ana aiwatar da keɓewa na nuclei ta hanyar ingantattun halittar cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cuta guda takwas tare da tsallakewa biyu. Within this system, gel beads incorporating barcodes, primers, enzymes, and a single nucleus are encapsulated in nanoliter-sized oil drops, forming Gel Bead-in-Emulsion (GEM). Wadannan nau'ikan daraja, sel na sumba da kuma sandararrawa ya faru a cikin kowane dutse. Bayan haka, kwayoyin daga Mrna sun ce fassarar aika rubuce rubucen CDNA, Hada Barcodes na 10X da kuma abubuwan ganowa na musamman (UMIs). Wadannan cdnas za su himmatu a cikin dakin karatu na daidaito, suna sauƙaƙe wani abu mai kyau da kuma cikakken bincike game da bayanan shaidar Gene a matakin Single-Single.

Dogara: 10 × Genomics Chromium Chromium da kuma dandamali Nalm

Tuntube mu

Bayanan sabis

Bioinformatics

Sakamakon demo

Littattafan da aka nuna

Tsarin fasaha

Ana samun kadaita na nuclei ta 10 × Genomic ™ Genomic ™, wanda ya ƙunshi tsarin microfluidar Microfluidics tare da giciye biyu. A cikin wannan tsarin, beads gel tare da barcoodes da na farko, enzymes da kuma enzymes da kuma enzymes da kuma enzymes da kuma enzymes da kuma enzymes da kuma enzymes da kuma enzymes da aka lullube a cikin dividus na nanoliter, yana samar da-sized mai-da-emulsion (Gel). Da zarar an kafa daraja, ƙwayoyin sel kuma sakin ma'aikatan ana yin su a cikin kowane dutse. Mrna ta juyar da kwayoyin halittar CDNA tare da sandacodes 10 × barcodes da Umi, wanda ke kara zama batun daidaitaccen tsarin ginin dakin aiki.

Fasas

● Shirye-shirye na dakatarwar nuclei daga kyallen kyallen

● samuwar gel bead-in-emulsion (datti) bi ta CDNA SYNthesis

● Kowanne bead a cikin dutse an ɗora shi da strimers wanda aka hada da sassan 4:

poly (DT) wutsiya ga Mista Pringing da CDNA Synthesis,

Na musamman kwayoyin cuta (UMI) don gyara ingantaccen amplification nuna

10X Barcode

ADDING SUKE SUARYA KARANTA CIGABA 1

Yan fa'idohu

Single-nucleus RNA SEQUCETS Karo ga iyakokin sequencing, ba da damar:

● Amfani da samfuran daskararre kuma ba wai kawai iyakance ga sabbin samfurori ba

Low low damuwa na sel mai sanyi lokacin da aka kwatanta da enzymatic magani na sabo ne 'sabo, nuna a cikin bayanan da aka sanya halittun tashin hankali

● Babu buƙatar cire kwalban jini

Norlimited celleter na mara iyaka

● Babban nau'ikan samfurori waɗanda suka cancanci bincike, gami da nau'ikan ƙwayar nama da ke haifar da clatsarancin clats

Samfures da ba za a iya bincika samfuran Somel Sequincing ba kuma sun cancanci yin seclei RNUBUWIGE:

Cell / nama	Dalili
Fruresh mai narkewa	An kasa samun sabbin kungiyoyi masu yawa ko da aka ajiye
Sashin tsoka, megararyocyte, mai ...	Duniyar tantanin halitta yayi yawa don shiga kayan aiki
Hearce ...	Too mai rauni don karya, ba zai iya bambance ƙwayoyin guda ɗaya ba
Cellar neuron, kwakwalwa ...	Mafi hankali, mai sauƙin damuwa, zai canza sakamakon takalmin
Pancreas, thyroid ...	Mawadaci a cikin enzensuus enzymes, wanda ya shafi samar da guda ɗaya

--Nucleus vs seadel

Guda-nucleus	Guda-sel
Diamici da Unlimited Cell Diamiter	Pel Duhun: 10-40 μm
Kayan za su iya zama nama mai sanyi	Kayan dole ne sabo ne sabo
Low damuwa na sel mai sanyi	Jiyya na Enzyme na iya haifar da karawar tantancewa
Babu sel mai launin ja da ake bukatar cire shi	Sel jini suna buƙatar cire
Nukiliya ta bayyana batun batun siyasa	Dukkanin sel ya bayyana batun bioinformation

Muhawara

Bukatun Samfura

Leburare

Tsarin Sequining

Bayanai da shawarar

Iko mai inganci

Lafiyar dabbobi ≥ 200 MG

Shuka nama ≥ 400 mg

10X Genomics sn cdna laburare

Illumina Pe150

100k pe Karanta a kowace tantanin halitta

(100-200 GB)

700-1200 nuclei / μL da nuclei mutuntaka lura a karkashin microscope

Don ƙarin cikakkun bayanai game da shirye-shiryen yin shiri da aikin motsa jiki, don Allah jin daɗin magana daMasanin BMkgene

Aikin aikin sabis

A baya: Shuka / dabbobi duk nau'in sequencing na gaske

Next: Kungiyar ta asali

Ya hada da bincike mai zuwa:

Ikon iko: Yawan sel, gano halittar sel, daidaitattun sel, kwayoyin sel da kuma Rana Kalmomi

● A cikin Bincike Imet:

Tsarin tantanin halitta da tari

Bayani daban-daban na bincike: Shaida na Dogs a cikin Gungu

Bayani mai aiki da wadatar da gungiyoyin cluster

Binciken Inter-Stations:

Hade bayanai

Bayani daban-daban na bincike: Shaida na Dogs a cikin kungiyoyi

Bayani mai aiki da wadatar da dogs na rukuni

Nazarin ci gaba:

Bincike na tantanin halitta

Bincike na Magana

Binciken Sadarwa (Selelphonedb)

Gane na kafa nazarin Ingantaccen bincike (GSEA)

Bincike na ciki

Cikin tantanin halitta:

Bayani na Bincike na Bincike: Dokokin Cung

Binciken Inter-Stration

Bayani na bincike daban-daban: Dogsungiyoyin rukuni

Bincike mai zurfi:

Bincike na Magane:

Binciken Celli:

Bincika ayyukan ci gaba da sabis na Bmkgene na RNA na RNOLEUUS By 10x Chromium a cikin wadannan littattafan da aka nuna:

Wang, L. et al. (2021) 'Binciken sirri na tantanin halitta ya nuna yanayin tsabtace na huhu a cikin Steroid-tsayayya da asmaAyyukan Kwalejin Kasa na Kimiyya ta Amurka ta Amurka, 118 (2), p. E2005590118. Doi: 10.1073 / pnas.2005590118

Zheng, H. et al. (2022) 'Cibiyar sadarwa ta duniya don Dyykulated Gene da kuma mahaukaciyar siginar rayuwa a cikin sel na rigakafi a cikin microenvoto na tsaran kursya',M a cikin ilimin rigakafi, 13, p. 879824. Doi: 10.3389 / Fimmu.20228.879824 / BOTTAX.

Tian, H. et al. (2023) 'Tallafin sihiri na Singercripters da Amintattun Leukocytes bayan alurar rigakafi Edwaration tare da Olichthys Olivaceus)',Ɗakin maliki, 566, p. 739238. Doi: 10.1016 / jam.aquacult.023.739238.

Yu, Y. et al. (2023) 'Tafiya ta Photodamic yana inganta sakamakon kawar da wuraren shakatawa ta hanyar rigakafi na togor a cikin marassa lafiya tare da cutar kansaGastic kamshi, 26 (5), PP. 798-813. doi: 10.1007 / s10120-023-01409-x / awo.