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BMKMANU S3000_Spatial Transcriptome
Ƙididdigar sararin samaniya yana tsaye a kan gaba na ƙirƙira ƙididdiga, yana ƙarfafa masu bincike don zurfafa cikin ƙirƙira tsarin bayyana kwayoyin halitta a cikin kyallen takarda yayin da suke kiyaye yanayin sararin samaniya. Tsakanin dandamali daban-daban, BMKGene ya haɓaka BMKManu S3000 Spatial Transcriptome Chip, yana alfahari da ingantaccen ƙuduri na 3.5µm, yana isa kewayon salula, da kunna saitunan ƙuduri masu yawa. Guntuwar S3000, wacce ke nuna kusan tabo miliyan 4, tana ɗaukar microwells ɗin da aka lulluɓe tare da beads ɗin da aka ɗora tare da ɓangarorin ɗaukar hoto. An shirya ɗakin karatu na cDNA, wanda aka wadatar da lambobin sirri, daga guntuwar S3000 kuma daga baya aka yi ta kan dandalin Illumina NovaSeq. Haɗuwa da samfuran barcoded na sararin samaniya da UMI suna tabbatar da daidaito da ƙayyadaddun bayanan da aka samar. Guntuwar BMKManu S3000 tana da matuƙar dacewa, tana ba da saitunan ƙuduri masu yawa waɗanda za'a iya daidaita su zuwa kyallen takarda daban-daban da matakan da ake so na daki-daki. Wannan daidaitawar tana sanya guntu a matsayin zaɓi na musamman don nazarin kwafi daban-daban na sararin samaniya, yana tabbatar da madaidaicin tari tare da ƙaramar amo. Amfani da fasahar rabuwar tantanin halitta tare da BMKManu S3000 yana ba da damar iyakance bayanan kwafi zuwa iyakokin sel, yana haifar da bincike wanda ke da ma'anar ilimin halitta kai tsaye. Bugu da ƙari, ingantacciyar ƙuduri na sakamakon S3000 a cikin mafi girman adadin kwayoyin halitta da UMI da aka gano ta tantanin halitta, yana ba da damar ingantaccen bincike na tsarin kwafin sararin samaniya da tari na sel.
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Single-Nuclei RNA Sequencing
Haɓaka kamun cell guda ɗaya da dabarun gina ɗakin karatu na al'ada, haɗe tare da babban tsari, ya kawo sauyi na nazarin maganganun kwayoyin halitta a matakin tantanin halitta. Wannan ci gaban yana ba da damar yin zurfafa da cikakken bincike game da hadaddun yawan adadin tantanin halitta, da shawo kan iyakokin da ke da alaƙa da matsakaicin magana akan dukkan sel da kuma kiyaye ainihin bambance-bambancen tsakanin waɗannan al'ummomi. Yayin da jerin RNA-cell guda ɗaya (scRNA-seq) yana da fa'idodi da ba za a iya musantawa ba, yana fuskantar ƙalubale a wasu kyallen takarda inda ƙirƙirar dakatarwar tantanin halitta guda ɗaya ke da wahala kuma yana buƙatar sabbin samfura. A BMKGene, muna magance wannan ƙalubalen ta hanyar ba da jerin RNA guda ɗaya (snRNA-seq) ta amfani da fasahar zamani ta 10X Genomics Chromium fasaha. Wannan hanya tana faɗaɗa nau'ikan samfuran da za a iya amfani da su don nazarin kwafi a matakin cell-ɗaya.
Keɓewar ƙwayoyin cuta ana samun su ta hanyar sabon guntu na 10X Genomics Chromium, wanda ke nuna tsarin microfluidics na tashoshi takwas tare da ketare biyu. A cikin wannan tsarin, gel beads wanda ya haɗa da barcodes, primers, enzymes, da kuma tsakiya guda ɗaya suna kunshe a cikin nau'in man fetur na nanoliter, suna samar da Gel Bead-in-Emulsion (GEM). Bayan samuwar GEM, sel lysis da sakin lambar sirri suna faruwa a cikin kowane GEM. Daga baya, kwayoyin mRNA suna juyar da rubutu zuwa cDNAs, suna haɗa lambobin barcode 10X da Abubuwan Gano Kwayoyin Halitta na Musamman (UMIs). Waɗannan cDNAs ana sa su zuwa daidaitattun ginin ɗakin karatu, suna sauƙaƙe bincike mai ƙarfi da cikakken bincike na bayanan bayanan kwayoyin halitta a matakin cell-guda.
Platform: 10× Genomics Chromium da Illumina NovaSeq Platform
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10x Genomics Visium Spatial Transcriptome
Fassarar fasikanci fasaha ce mai yanke hukunci wacce ke ba masu bincike damar bincika tsarin maganganun kwayoyin halitta a cikin kyallen takarda yayin da suke kiyaye yanayin sararin samaniya. Ɗayan dandamali mai ƙarfi a cikin wannan yanki shine 10x Genomics Visium haɗe tare da jerin Illumina. Ka'idar 10X Visium ta ta'allaka ne akan guntu na musamman tare da yankin da aka keɓe inda aka sanya sassan nama. Wannan yanki na kama yana ƙunshe da ɓangarorin ɓangarorin, kowanne ya yi daidai da wani wuri na musamman a cikin nama. Kwayoyin RNA da aka kama daga nama ana yi musu lakabi da kebantattun abubuwan gano kwayoyin halitta (UMIs) yayin aiwatar da rubutun baya. Waɗannan tabobin da aka ƙera da UMI suna ba da damar taswirar taswirar sararin samaniya da ƙididdige yawan magana a cikin ƙudurin tantai ɗaya. Haɗuwa da samfuran barcoded na sararin samaniya da UMI suna tabbatar da daidaito da ƙayyadaddun bayanan da aka samar. Ta amfani da wannan fasaha ta Spatial Transcriptomics, masu bincike za su iya samun zurfin fahimta game da tsarin sararin samaniya na sel da hadaddun hulɗar kwayoyin da ke faruwa a cikin kyallen takarda, suna ba da haske mai mahimmanci game da hanyoyin da ke tattare da tsarin ilimin halitta a fannoni da yawa, ciki har da oncology, neuroscience, ilmin halitta na ci gaba, rigakafi. , da kuma nazarin halittu.
Platform: 10X Genomics Visium da Illumina NovaSeq
