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Kwatanta Genomics
Kwatanta kwayoyin halitta sun haɗa da bincike da kwatanta dukkanin jerin kwayoyin halitta da sifofi a tsakanin nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan halittu ne. Wannan filin yana neman bayyana juyin halittar nau'in, yanke ayyukan kwayoyin halitta, da kuma bayyana hanyoyin sarrafa kwayoyin halitta ta hanyar gano tsare-tsaren tsare-tsaren tsare-tsare ko mabanbanta da abubuwa a cikin halittu daban-daban. Cikakken binciken kwatancen genomics ya ƙunshi nazari kamar dangin jinsin halitta, haɓakar juyin halitta, al'amuran kwafi gaba ɗaya, da tasirin matsi na zaɓi.
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Juyin Halitta
An kafa dandalin tantance yawan jama'a da juyin halitta bisa ga dimbin gogewa da aka tara a cikin tawagar BMK R&D tsawon shekaru. Kayan aiki ne mai sauƙin amfani musamman ga masu bincike waɗanda ba su da mahimmanci a cikin bioinformatics. Wannan dandali yana ba da damar ainihin ilimin halittar ɗan adam da ke da alaƙa da bincike na asali wanda ya haɗa da ginin bishiyar phylogenetic, bincike na rashin daidaituwa na haɗin gwiwa, ƙimayar bambancin kwayoyin halitta, binciken share fage, nazarin dangi, PCA, nazarin tsarin yawan jama'a, da sauransu.
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Hi-C Based Genome Assembly
Hi-C wata hanya ce da aka ƙera don ɗaukar daidaitawar chromosome ta hanyar haɗa ma'amala ta tushen kusanci da babban tsarin aiwatarwa. An yi imanin ƙarfin waɗannan hulɗar yana da alaƙa mara kyau tare da nisa ta jiki akan chromosomes. Don haka, ana amfani da bayanan Hi-C don jagorar tari, oda, da daidaita jerin abubuwan da aka taru a cikin daftarin kwayoyin halitta da kuma rataya waɗancan kan takamaiman adadin chromosomes. Wannan fasaha tana ba da ƙarfin taro na matakin chromosome a cikin rashin taswirar jinsin jama'a. Kowane genome guda ɗaya yana buƙatar Hi-C.
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Tsarin Shuka/ Dabba De Novo Genome Sequencing
Da Novosequencing yana nufin gina nau'in 'genome' gaba ɗaya ta amfani da fasahohin jeri a cikin rashi kwayoyin halitta. Gabatarwa da yaɗuwar karɓowar jerin tsararraki na ƙarni na uku, waɗanda ke nuna tsayin karatu, sun haɓaka haɗaɗɗun kwayoyin halitta sosai ta hanyar haɓaka haɗuwa tsakanin karantawa. Wannan haɓakawa yana da mahimmanci musamman lokacin da ake fuskantar ƙalubalen ƙwayoyin cuta, kamar waɗanda ke nuna babban heterozygosity, babban rabo na yankuna masu maimaitawa, polyploids, da yankuna tare da abubuwa masu maimaitawa, abubuwan da ke cikin GC mara kyau, ko babban hadaddun waɗanda galibi ba su taru ta amfani da jerin gajerun karantawa. kadai.
Maganin mu na tsayawa ɗaya yana ba da sabis na haɗaɗɗen jeri da bincike na bioinformatic wanda ke ba da ingantaccen tsarin de novo haɗe-haɗe. Binciken farko na genome tare da Illumina yana ba da ƙididdiga na girman kwayoyin halitta da rikitarwa, kuma ana amfani da wannan bayanin don jagorantar mataki na gaba na jerin dogon karantawa tare da PacBio HiFi, wanda ya biyo baya.da novotaro na contigs. Yin amfani da taron HiC na gaba yana ba da damar ɗora ƙugiya zuwa kwayoyin halitta, samun taron matakin-chromosome. A ƙarshe, ana tantance kwayar halittar ta hanyar tsinkayar kwayoyin halitta da kuma jera nau'ikan halittun da aka bayyana, da yin amfani da rubutattun rubuce-rubuce tare da gajeru da dogon karantawa.
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Tsarin Dan Adam Gabaɗaya Exome
Human Whole exome sequencing (hWES) an yarda da shi azaman hanya mai inganci mai tsada kuma mai ƙarfi don tantance maye gurbi masu haifar da cuta. Duk da kasancewar kusan kashi 1.7% na dukkan kwayoyin halitta, exons suna taka muhimmiyar rawa ta hanyar nuna bayanan ayyukan furotin kai tsaye. Musamman ma, a cikin kwayoyin halittar ɗan adam, sama da kashi 85% na maye gurbi masu alaƙa da cututtuka suna bayyana a cikin yankunan da ake shigar da furotin. BMKGENE yana ba da cikakkiyar sabis na jeri na ɗan adam mai sassauƙa tare da dabarun ɗaukar dabaru daban-daban guda biyu don cimma burin bincike daban-daban.
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Takamaiman-Locus Amplified Sequencing Sequencing (SLAF-Seq)
Babban aikin genotyping, musamman akan yawan jama'a, mataki ne na asali a cikin nazarin ƙungiyar kwayoyin halitta kuma yana ba da tushen tushen kwayoyin halitta don gano kwayoyin halittar aiki, nazarin juyin halitta, da sauransu. Maimakon zurfin tsarin sake fasalin kwayoyin halitta,Rage Wakilci Tsarin Tsarin Halittu (RRGS)galibi ana amfani da shi a cikin waɗannan karatun don rage ƙimar jeri kowane samfuri yayin da ake ci gaba da ingantaccen aiki a cikin gano alamar kwayoyin halitta. RRGS yana samun wannan ta hanyar narkar da DNA tare da ƙuntatawa enzymes da kuma mai da hankali kan takamaiman girman girman guntu, ta haka ne ke tsara juzu'i na kwayoyin halitta. Daga cikin hanyoyin RRGS iri-iri, Specific-Locus Amplified Fragment Sequencing (SLAF) tsari ne na musamman da inganci. Wannan hanyar, wacce BMKGene ta haɓaka ta kanta, tana haɓaka ƙayyadaddun enzyme da aka saita don kowane aiki. Wannan yana tabbatar da samar da adadi mai yawa na alamun SLAF (yankin 400-500 bps na genome da ake jera su) waɗanda aka rarraba iri ɗaya a cikin kwayoyin halitta yayin da suke guje wa yankuna masu maimaitawa yadda ya kamata, don haka yana tabbatar da mafi kyawun gano alamun kwayoyin halitta.
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Illumina da aka riga aka yi dakunan karatu
Fasahar sequencing na Illumina, dangane da Sequencing by Synthesis (SBS), ƙirƙira ce ta NGS wacce ta karɓi duniya gabaɗaya, alhakin samar da sama da kashi 90% na bayanan jeri na duniya. Ƙa'idar SBS ta ƙunshi ɗaukar hoto mai lakabi mai jujjuyawa mai jujjuyawa yayin da aka ƙara kowane dNTP, kuma daga baya an raba shi don ba da damar haɗa tushe na gaba. Tare da duk dNTPs guda huɗu masu jujjuyawa masu ɗaure da ke akwai a cikin kowane zagayowar zagayowar, gasa ta yanayi tana rage son haɗawa. Wannan fasaha mai jujjuyawar tana goyan bayan dakunan karatu guda-ɗaya da masu haɗa-zuwa-ƙarshe, suna ba da kewayon aikace-aikacen kwayoyin halitta. Illumina sequencing's high-fid caparfients da madaidaicin sanya shi a matsayin ginshiƙi a cikin binciken kimiyyar halittu, ƙarfafa masana kimiyya don buɗe ɓarna na kwayoyin halitta tare da cikakkun bayanai da inganci.
Sabis ɗin jeri na ɗakin karatu da aka riga aka yi yana bawa abokan ciniki damar shirya dakunan karatu daga tushe daban-daban (mRNA, gabaɗayan genome, amplicon, ɗakunan karatu na 10x, da sauransu). Daga baya, waɗannan ɗakunan karatu za a iya jigilar su zuwa cibiyoyin jerin abubuwan mu don kula da inganci da tsari a dandamalin Illumina.
