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Tsarin Metagenomic -NGS
Metagenome tarin jimillar kwayoyin halitta na gaurayawan al'umman halittu, kamar muhalli da metagenomes na mutum. Ya ƙunshi kwayoyin halitta na ƙwayoyin cuta da ake iya nomawa da waɗanda ba za a iya nomawa ba. Tsarin metagenomic na Shotgun tare da NGS yana ba da damar yin nazarin waɗannan ƙayyadaddun yanayin yanayin yanayin halitta wanda aka haɗa cikin samfuran muhalli ta hanyar samar da fiye da bayanan haraji, yana ba da ƙarin haske game da bambance-bambancen nau'in, haɓakar haɓaka, da hadaddun tsarin yawan jama'a. Bayan nazarin haraji, metagenomics na harbin bindiga kuma yana ba da hangen nesa mai aiki na genomics, yana ba da damar bincikar ƙwayoyin halittar da aka sanya su da rawar da suke takawa a cikin tsarin muhalli. A ƙarshe, kafa hanyoyin sadarwa tsakanin abubuwan halitta da abubuwan muhalli suna ba da gudummawa ga cikakkiyar fahimtar ma'amala tsakanin ƙananan ƙwayoyin cuta da asalinsu. A ƙarshe, jeri na metagenomic yana tsaye a matsayin kayan aiki mai mahimmanci don buɗe ɓoyayyiyar ɓarna na al'ummomi daban-daban na ƙananan ƙwayoyin cuta, yana haskaka alaƙa mai ban sha'awa tsakanin jinsin halittu da ilimin halittu a cikin waɗannan sarƙaƙƙiyar yanayin halittu.
Platform: Illumina NovaSeq da DNBSEQ-T7
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Tsarin Metagenomic-TGS
Metagenome tarin kwayoyin halitta ne na gaurayawan al'umman halittu, kamar muhalli da metagenomes na mutum. Ya ƙunshi kwayoyin halitta na ƙwayoyin cuta da ake iya nomawa da waɗanda ba za a iya nomawa ba. Tsarin metagenomic yana ba da damar yin nazarin waɗannan ƙayyadaddun yanayin yanayin yanayin halitta wanda aka haɗa a cikin samfuran muhalli ta hanyar samar da fiye da bayanan haraji. Hakanan yana ba da hangen nesa mai aiki na genomics ta hanyar binciko ɓoyayyun kwayoyin halitta da ayyukansu na sakawa a cikin tsarin muhalli. Duk da yake an yi amfani da tsarin bindigu na gargajiya tare da jerin Illumina a cikin nazarin metagenomic, zuwan Nanopore da PacBio jerin dogon karantawa sun canza filin. Fasahar Nanopore da PacBio suna haɓaka bincike na bioinformatic na ƙasa, musamman taron metagenome, yana tabbatar da ƙarin ci gaba da taruka. Rahotanni sun nuna cewa tushen Nanopore da PacBio na tushen metagenomics sun sami nasarar samar da cikakkun kuma rufaffiyar kwayoyin halittar kwayoyin cuta daga hadadden microbiomes (Moss, EL, et al., Nature Biotech, 2020). Haɗa karatun Nanopore tare da karanta Illumina yana ba da dabarar dabara don gyara kuskure, rage ƙarancin daidaiton ainihin Nanopore. Wannan haɗin haɗin gwiwar yana ba da ƙarfi na kowane dandali na jeri, yana ba da mafita mai ƙarfi don shawo kan yuwuwar iyakoki da haɓaka daidaito da amincin ƙididdigar metagenomic.
Platform: Nanopore PromethION 48, Illumina da PacBio Revio
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Gabaɗaya jerin kwayoyin halitta bisulfite (WGBS)
Duka Genome Bisulfite Sequencing (WGBS) yana tsaye a matsayin tsarin daidaitaccen zinari don zurfin bincike na DNA methylation, musamman matsayi na biyar a cikin cytosine (5-mC), mai mahimmanci mai daidaita maganganun kwayoyin halitta da ayyukan salula. Ka'idar da ke ƙarƙashin WGBS ta ƙunshi jiyya na bisulfite, haifar da jujjuyawar cytosines marasa daidaituwa zuwa uracil (C zuwa U), yayin barin cytosin methylated ba canzawa. Wannan dabarar tana ba da ƙuduri mai tushe guda ɗaya, yana bawa masu bincike damar bincikar methylome gabaɗaya kuma su gano tsarin methylation mara kyau da ke da alaƙa da yanayi daban-daban, musamman kansa. Ta hanyar amfani da WGBS, masana kimiyya za su iya samun haske mara misaltuwa cikin yanayin shimfidar wurare masu faɗi na methylation, suna ba da cikakkiyar fahimta game da hanyoyin epigenetic waɗanda ke haifar da matakai da cututtuka iri-iri.
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Ƙididdiga don Canjawa-Sarfafa Chromatin tare da Babban Sabis na Sabis (ATAC-seq)
ATAC-seq babbar dabara ce ta jerin abubuwan da aka yi amfani da ita don nazarin isa ga chromatin mai faɗin kwayoyin halitta. Ana amfani da shi yana ba da zurfin fahimta game da hadaddun hanyoyin sarrafa epigenetic na duniya akan maganganun kwayoyin halitta. Hanyar tana amfani da jujjuyawar Tn5 mai ƙarfi zuwa ga guntu lokaci guda da yiwa buɗaɗɗen yankuna chromatin ta saka adaftan jeri. Sakamakon haɓakawa na PCR na gaba a cikin ƙirƙirar ɗakin karatu mai ɗorewa, wanda ke ba da damar gano cikakkiyar ganewa na yankuna chromatin da ke buɗe ƙarƙashin takamaiman yanayi-lokaci. ATAC-seq yana ba da cikakkiyar ra'ayi na shimfidar wurare na chromatin mai isa, sabanin hanyoyin da ke mayar da hankali kawai kan wuraren ɗaurin rubutu ko takamaiman yankuna da aka gyara na tarihi. Ta hanyar jera waɗannan yankuna na chromatin da ke buɗe, ATAC-seq yana bayyana yankuna mafi yuwuwar samun jerin tsari na aiki da yuwuwar abubuwan da ke ɗaure rubutu, suna ba da fa'ida mai mahimmanci game da haɓakar yanayin yanayin magana a cikin kwayoyin halitta.
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16S/18S/ITS Amplicon Sequencing-PacBio
Kwayoyin halittar 16S da 18S rRNA, tare da yankin Internal Transcribed Spacer (ITS), suna aiki a matsayin alamomin zanen yatsa na kwayoyin halitta saboda hadewarsu na yankuna da aka kiyaye sosai da masu saurin canzawa, suna sanya su kayan aiki masu mahimmanci don siffanta kwayoyin prokaryotic da eukaryotic. Ƙwaƙwalwa da jeri na waɗannan yankuna suna ba da hanyar da ba ta da keɓancewa don bincika ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun yanayin halittu daban-daban. Yayin da jerin abubuwan Illumina yawanci ke kaiwa ga gajerun yankuna masu canzawa kamar V3-V4 na 16S da ITS1, an nuna cewa ana iya samun ingantaccen bayanin haraji ta hanyar yin cikakken tsayin 16S, 18S, da ITS. Wannan cikakkiyar dabarar tana haifar da mafi girman kashi na daidaitattun jeri-jeri, cimma matakin ƙuduri wanda ya kai ga tantance nau'in. PacBio's Single-Molecule Real-Time Real-Time (SMRT) dandali na jeri ya fice ta hanyar samar da ingantaccen dogon karantawa (HiFi) wanda ke rufe cikakken amplicons, yana fafatawa da madaidaicin jerin Illumina. Wannan damar tana bawa masu bincike damar samun fa'ida mara misaltuwa - ra'ayi mai ban mamaki game da yanayin yanayin halitta. Tsawaita ɗaukar hoto yana haɓaka ƙuduri sosai a cikin bayanin nau'ikan, musamman a cikin al'ummomin ƙwayoyin cuta ko na fungal, yana ba da damar fahimtar zurfafa fahimtar rikice-rikice na yawan ƙananan ƙwayoyin cuta.
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16S/18S/ITS Amplicon Sequencing-NGS
Amplicon sequencing tare da fasahar Illumina, musamman niyya 16S, 18S, da alamomin kwayoyin halitta ITS, hanya ce mai ƙarfi don buɗe phylogeny, taxonomy, da nau'ikan nau'ikan halittu a cikin al'ummomin ƙananan ƙwayoyin cuta. Wannan hanya ta ƙunshi jerin yankuna masu saurin canzawa na alamomin kwayoyin halitta. Asali an gabatar dashi azaman sawun yatsa na kwayoyin halitta taWahala et ala cikin 1977, wannan dabarar ta canza bayanin martabar microbiome ta hanyar ba da damar yin nazari mara keɓewa. Ta hanyar jeri na 16S (kwayoyin cuta), 18S (fungi), da Internal Transcribed Spacer (ITS, fungi), masu bincike za su iya gano ba kawai nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau’in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in), wadanda ba a san su ba da kuma wadanda ba a tantance su ba. An karɓe shi sosai azaman kayan aiki mai mahimmanci, jerin amplicon ya zama kayan aiki don gano nau'ikan ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun abubuwa a wurare daban-daban, gami da bakin ɗan adam, hanji, stool, da ƙari.
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Bacterial da Fungal Dukan Halittun Halittu Sake-Sequencing
Bacterial da fungal dukan-genome re-sequencing ayyukan suna da mahimmanci don haɓaka ƙananan ƙwayoyin cuta ta hanyar ba da damar kammalawa da kwatanta ƙwayoyin ƙwayoyin cuta. Wannan yana sauƙaƙe aikin injiniya na fermentation, haɓaka hanyoyin masana'antu, da kuma bincika hanyoyin metabolism na biyu. Bugu da ƙari, sake-sake tsarin naman gwari da ƙwayoyin cuta yana da mahimmanci don fahimtar daidaita yanayin muhalli, haɓaka nau'ikan iri, da bayyanar da yanayin juyin halitta, tare da fa'ida mai fa'ida a cikin magani, aikin gona, da kimiyyar muhalli.
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PacBio-Full-tsawon 16S/18S/ITS Tsarin Amplicon
Amplicon (16S / 18S / ITS) dandamali an haɓaka shi tare da shekaru na gwaninta a cikin nazarin ayyukan bambance-bambancen microbial, wanda ya ƙunshi daidaitaccen bincike na asali da bincike na keɓaɓɓu: bincike na asali ya ƙunshi babban abun ciki na bincike na bincike na microbial na yanzu, abun ciki na bincike yana da wadata kuma cikakke. kuma ana gabatar da sakamakon bincike ta hanyar rahotannin aikin; Abubuwan da ke cikin keɓaɓɓen bincike sun bambanta. Za'a iya zaɓar samfurori kuma ana iya saita sigogi cikin sassauƙa bisa ga ainihin rahoton bincike da manufar bincike, don gane keɓaɓɓen buƙatun. Tsarin aiki na Windows, mai sauƙi da sauri.
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Rubutun Rubutun-cikakken-PacBio (Ba Nasiha ba)
Ɗaukar bayanan jeri na Biosciences na Pacific (PacBio) Isoform a matsayin shigarwa, wannan App ɗin yana da ikon gano jerin cikakkun bayanan rubutu (ba tare da taro ba). Ta hanyar yin taswirar cikakken tsayin daka akan kwayoyin halitta, ana iya inganta kwafin ta hanyar sanannun kwayoyin halitta, kwafi, yankuna masu coding, da sauransu. Binciken haɗin gwiwa tare da bayanan bayanan kwafi na NGS yana ba da damar ƙarin cikakkun bayanai da ƙarin ƙididdige ƙididdigewa a cikin magana a matakin kwafi, wanda ya fi fa'ida fa'ida ta asali da kuma nazarin ayyuka.
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Rage Matsayin Bisulfite Sequencing (RRBS)
Rage Wakilci Bisulfite Sequencing (RRBS) ya fito a matsayin mai inganci mai tsada da ingantaccen madadin Tsarin Tsarin Halittu na Halitta (WGBS) a cikin binciken DNA methylation. Yayin da WGBS ke ba da cikakkun bayanai ta hanyar nazarin dukkan kwayoyin halitta a ƙudurin tushe guda ɗaya, ƙimar sa mai girma na iya zama abin iyakancewa. RRBS da dabara yana rage wannan ƙalubalen ta hanyar zaɓin nazarin sashin wakilci na kwayoyin halitta. Wannan dabarar ta dogara ne akan haɓaka yankuna masu wadatar tsibiri na CpG ta hanyar tsagewar MspI wanda ke biye da girman zaɓi na 200-500/600 bps. Sakamakon haka, kawai yankuna masu kusanci zuwa tsibiran CpG ne aka jera su, yayin da waɗanda ke da tsibiran CpG masu nisa ba a cire su daga binciken. Wannan tsari, haɗe tare da bisulfite sequencing, yana ba da damar gano babban ƙuduri na DNA methylation, da kuma tsarin tsarin, PE150, yana mai da hankali musamman a kan ƙarshen abubuwan da aka saka maimakon tsakiya, yana ƙara haɓakar haɓakar methylation. RRBS kayan aiki ne mai mahimmanci wanda ke ba da damar bincike na DNA methylation mai tsada mai tsada da haɓaka ilimin hanyoyin epigenetic.
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Prokaryotic RNA Sequencing
Jeri na RNA yana ba da ikon cikakken bayanin duk bayanan RNA a cikin sel a ƙarƙashin takamaiman yanayi. Wannan fasaha mai yanke-yanke tana aiki azaman kayan aiki mai ƙarfi, yana buɗe bayanan bayanan ƙwayoyin cuta masu rikitarwa, tsarin halittar halitta, da hanyoyin ƙwayoyin ƙwayoyin cuta masu alaƙa da nau'ikan hanyoyin nazarin halittu. An karɓe shi sosai cikin bincike na asali, bincike na asibiti, da haɓaka magunguna, jerin RNA yana ba da haske game da ɓarna na haɓakar salon salula da ka'idojin kwayoyin halitta. Samfurin samfurin mu na prokaryotic RNA an keɓance shi don kwafi na prokaryotic, wanda ya haɗa da raguwar rRNA da shirye-shiryen ɗakin karatu na jagora.
Platform: Illumina NovaSeq
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Metatranscriptome Sequencing
Yin amfani da fasahar sequencing na Illumina, BMKGENE's metatranscriptome sequencing sabis yana buɗewa da ƙarfin magana mai ƙarfi na nau'ikan microbes iri-iri, yaɗa eukaryotes zuwa prokaryotes da ƙwayoyin cuta, a cikin yanayin yanayi kamar ƙasa, ruwa, teku, stool, da hanji. Cikakken sabis ɗinmu yana ƙarfafa masu bincike don zurfafa cikin ƙayyadaddun bayanan bayanan ƙwayoyin ƙwayoyin cuta masu rikitarwa. Bayan nazarin lissafin haraji, sabis ɗin mu na metatranscriptome yana sauƙaƙe bincike cikin haɓaka aiki, yana ba da haske akan nau'ikan da aka bayyana daban-daban da kuma matsayinsu. Gano ɗimbin fa'idodin ilimin halitta yayin da kuke zagayawa cikin rikitattun shimfidar wurare na maganganun jinsi, bambancin haraji, da haɓaka aiki a cikin waɗannan ɗimbin mahalli.
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De novo Fungal Genome Assembly
BMKGENE yana ba da mafita iri-iri don kwayoyin fungal, yana biyan buƙatun bincike iri-iri da cikar kwayoyin halittar da ake so. Yin amfani da ɗan gajeren karanta jerin Illumina kaɗai yana ba da damar ƙirƙirar daftarin kwayoyin halitta. Gajerun karantawa da jerin dogon karantawa ta amfani da Nanopore ko Pacbio an haɗa su don ƙarin ingantaccen kwayar halittar fungi mai tsayi mai tsayi. Bugu da ƙari, haɗa jerin abubuwan Hi-C yana ƙara haɓaka iyawa, yana ba da damar samun cikakkiyar matakin chromosome-genome.
