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BMKMANU S3000_Spatial Transcriptome
Ƙididdigar sararin samaniya yana tsaye a kan gaba na ƙirƙira ƙididdiga, yana ƙarfafa masu bincike don zurfafa cikin ƙirƙira tsarin bayyana kwayoyin halitta a cikin kyallen takarda yayin da suke kiyaye yanayin sararin samaniya. Tsakanin dandamali daban-daban, BMKGene ya haɓaka BMKManu S3000 Spatial Transcriptome Chip, yana alfahari da ingantaccen ƙuduri na 3.5µm, yana isa kewayon salula, da kunna saitunan ƙuduri masu yawa. Guntuwar S3000, wacce ke nuna kusan tabo miliyan 4, tana ɗaukar microwells ɗin da aka lulluɓe tare da beads ɗin da aka ɗora tare da ɓangarorin ɗaukar hoto. An shirya ɗakin karatu na cDNA, wanda aka wadatar da lambobin sirri, daga guntuwar S3000 kuma daga baya aka yi ta kan dandalin Illumina NovaSeq. Haɗuwa da samfuran barcoded na sararin samaniya da UMI suna tabbatar da daidaito da ƙayyadaddun bayanan da aka samar. Guntuwar BMKManu S3000 tana da matuƙar dacewa, tana ba da saitunan ƙuduri masu yawa waɗanda za'a iya daidaita su zuwa kyallen takarda daban-daban da matakan da ake so na daki-daki. Wannan daidaitawar tana sanya guntu a matsayin zaɓi na musamman don nazarin kwafi daban-daban na sararin samaniya, yana tabbatar da madaidaicin tari tare da ƙaramar amo. Amfani da fasahar rabuwar tantanin halitta tare da BMKManu S3000 yana ba da damar iyakance bayanan kwafi zuwa iyakokin sel, yana haifar da bincike wanda ke da ma'anar ilimin halitta kai tsaye. Bugu da ƙari, ingantacciyar ƙuduri na sakamakon S3000 a cikin mafi girman adadin kwayoyin halitta da UMI da aka gano ta tantanin halitta, yana ba da damar ingantaccen bincike na tsarin kwafin sararin samaniya da tari na sel.
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DNBSEQ dakunan karatu da aka riga aka yi
DNBSEQ, wanda MGI ya haɓaka, sabuwar fasaha ce ta NGS wacce ta yi nasarar rage raguwar farashin jeri da haɓaka kayan aiki. Shirye-shiryen dakunan karatu na DNBSEQ ya ƙunshi rarrabuwar DNA, shirye-shiryen ssDNA, da haɓaka da'ira don samun DNA nanoballs (DNB). Ana ɗora waɗannan a kan ƙaƙƙarfan wuri kuma daga baya ana yin su ta hanyar haɗakar Binciken-Anchor Synthesis (cPAS). Fasahar DNBSEQ ta haɗu da fa'idodin samun ƙarancin ƙarar kuskuren haɓakawa tare da yin amfani da ƙirar ƙima mai yawa tare da nanoballs, yana haifar da jeri tare da mafi girma kayan aiki da daidaito.
Sabis ɗin jeri na ɗakin karatu da aka riga aka yi yana bawa abokan ciniki damar shirya ɗakunan karatu na Illumina daga mabambanta daban-daban (mRNA, dukkan kwayoyin halitta, amplicon, ɗakunan karatu na 10x, da sauransu), waɗanda aka canza zuwa ɗakunan karatu na MGI a cikin dakunan gwaje-gwajenmu don a jera su a cikin DNBSEQ-T7, suna ba da damar. babban adadin bayanai a ƙananan farashi.
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Hi-C tushen hulɗar Chromatin
Hi-C wata hanya ce da aka ƙera don ɗaukar daidaitawar kwayoyin halitta ta hanyar haɗa ma'amala ta tushen kusanci da babban tsarin aiwatarwa. Hanyar ta dogara ne akan chromatin crosslinking tare da formaldehyde, biye da narkewa da sake sakewa ta hanyar da kawai gutsuttsura waɗanda ke da alaƙa da haɗin gwiwa zasu samar da samfuran ligation. Ta hanyar tsara waɗannan samfuran ligation, yana yiwuwa a yi nazarin ƙungiyar 3D na genome. Hi-C yana ba da damar nazarin rarraba sassan kwayoyin halittar da aka cika da sauƙi (A compartments, euchromatin) kuma mafi kusantar yin aiki a rubuce, da kuma yankunan da suka fi cunkoso (B compartments, Heterochromatin). Hakanan ana iya amfani da Hi-C don nunawa Topologically Associated Domains (TADs), yankuna na genome waɗanda suka naɗe da tsarin kuma suna iya samun nau'ikan maganganu iri ɗaya, da kuma gano madaukai na chromatin, yankuna DNA waɗanda sunadaran sunada alaƙa da su kuma waɗanda suke. sau da yawa wadatar da abubuwa masu tsari. Sabis ɗin jerin abubuwan Hi-C na BMKGene yana ba masu bincike damar bincika sararin sararin samaniya na genomics, buɗe sabbin hanyoyi don fahimtar ƙa'idodin kwayoyin halitta da tasirinsa a cikin lafiya da cuta.
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TGuide Smart Magnetic Shuka RNA Kit
TGuide Smart Magnetic Shuka RNA Kit
Tsarkake jimlar RNA mai inganci daga kyallen shuka
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TGuide Smart Blood/Cell/Tissue RNA Kit
TGuide Smart Blood/Cell/Tissue RNA Kit
Kayan da aka cika da harsashi / farantin reagent kit don tsarkakewa mai girma, tsafta, inganci mai inganci, mara hanawa gabaɗaya RNA daga naman dabba/cell/sabon jini gaba ɗaya.
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TGuide Smart Magnetic Shuka DNA Kit
TGuide Smart Magnetic Shuka DNA Kit
Tsarkake DNA mai inganci mai inganci daga kyallen shuka iri-iri
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TGuide Smart Soil / Kit ɗin DNA
TGuide Smart Soil / Kit ɗin DNA
Yana tsarkake DNA marar hana hanawa na tsafta da inganci daga ƙasa da samfuran stool
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TGuide Smart DNA Tsaftace Kit
Yana dawo da ingantaccen DNA daga samfurin PCR ko gels agarose.
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TGuide Smart Blood Genomic DNA Kit
TGuide Smart Blood Genomic DNA Kit
Kayan da aka cika da harsashi / farantin reagent kit don tsarkakewar DNA daga jini da rigar buffy
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TGuide Smart Magnetic Tissue DNA Kit
Kayan da aka cika da harsashi / farantin reagent kit don fitar da kwayoyin halittar DNA daga kyallen dabba
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TGuide Smart Universal DNA Kit
Kayan da aka cika kwandon / farantin reagent kit don tsarkakewa kwayoyin halittar DNA daga jini, busasshen tabo na jini, kwayoyin cuta, sel, yau, swabs na baka, kyallen dabbobi, da sauransu.
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TGuide S16 Nucleic Acid Extractor
TGuide S16 Nucleic Acid Extractor
Kayan aikin Benchtop mai sauƙin amfani, Samfura 1-8 Ko 16 A lokaci guda
Lambar kasida / marufi
Cat. a'a
ID
No na preps
OSE-S16-AM
1 saiti
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PacBio 2+3 Cikakken Tsawon mRNA Magani
Yayin da tsarin mRNA na tushen NGS kayan aiki ne mai dacewa don ƙididdige maganganun kwayoyin halitta, dogaro da gajeriyar karantawa yana taƙaita tasirin sa a cikin hadaddun nazarin kwafin rubutu. A gefe guda, PacBio sequencing (Iso-Seq) yana amfani da fasahar dogon karantawa, yana ba da damar jeri cikakken kwafin mRNA. Wannan tsarin yana sauƙaƙe bincike mai zurfi na madadin splicing, gene fusions, da poly-adenylation, ko da yake ba shine zaɓi na farko don ƙididdige maganganun kwayoyin halitta ba. Haɗin 2 + 3 yana ƙaddamar da rata tsakanin Illumina da PacBio ta hanyar dogaro da PacBio HiFi yana karantawa don gano cikakken saitin isoforms na kwafi da jerin NGS don ƙididdige isoforms iri ɗaya.
Platform: PacBio Sequel II/ PacBio Revio da Illumina NovaSeq;
