Tsarin Tsiro/Dabbobi Gabaɗayan Halitta
Siffofin Sabis
● Shirye-shiryen ɗakin karatu na iya zama daidaitattun ko PCR
● Akwai a cikin 4 tsarin dandamali: Illumina NovaSeq, MGI T7, Nanopore Promethion P48, ko PacBio Revio.
● Binciken bioinformatic ya mayar da hankali kan gano bambance-bambancen: SNP, InDel, SV da CNV
Amfanin Sabis
●Ƙwararrun Ƙwararru da Rubuce-rubucen Buga: Ƙwarewar da aka tara a cikin jerin kwayoyin halitta don fiye da nau'in 1000 ya haifar da fiye da 1000 da aka buga tare da tasiri mai tasiri fiye da 5000.
●Cikakken Binciken Bioinformatics: Haɗe da bambancin kiran kira da bayanin aiki.
● Tallafin Bayan-tallace-tallace:Alƙawarinmu ya wuce bayan kammala aikin tare da lokacin sabis na watanni 3 bayan-sayar. A wannan lokacin, muna ba da bibiyar aikin, taimako na warware matsala, da kuma zaman Q&A don magance duk wata tambaya da ta shafi sakamakon.
●Cikakken Bayani: Muna amfani da ma'ajin bayanai da yawa don yin aikin tantance kwayoyin halitta tare da bambance-bambancen da aka gano da kuma yin nazarin haɓakar haɓaka, samar da haske kan ayyukan bincike da yawa.
Ƙayyadaddun Sabis
| Bambance-bambancen da za a gano | Dabarun jeri | Shawarwari zurfin |
| SNP da InDel | Illumina NovaSeq PE150 ya da MGI T7 | 10x ku |
| SV da CNV (ƙasa daidai) | 30x ku | |
| SV da CNV (mafi daidaito) | Nanopore Prom P48 | 20x ku |
| SNPs, Indels, SV da CNV | PacBio Revio | 10x ku |
Samfuran Bukatun
| Nama ko fitar da nucleic acid | Illumina/MGI | Nanopore | PacBio
| ||
| Animal Viscera | 0.5-1 g | 3.5 g
| 3.5 g
| ||
| tsokar dabba | 5g ku
| 5g ku
| |||
| Jinin Mammaliyan | 1.5 ml | 0.5 ml na ruwa
| ruwa 5 ml
| ||
| Jinin Kaji/Kifi | 0.1 ml na ruwa
| 0.5 ml na ruwa
| |||
| Shuka- Fresh Leaf | 1-2 g | 2 g ku
| 5g ku
| ||
| Kwayoyin Al'ada |
| 1 x107
| 1 x108
| ||
| Kwarin taushi nama / Mutum | 0.5-1 g | ku 1 g
| 3g ku
| ||
| Cire DNA
| Matsakaicin hankali: ≥ 1 ng/µL Adadi: ≥ 30 ng Iyakance ko babu lalacewa ko gurɓatawa
| Hankali Adadin
OD260/280
OD260/230
Iyakance ko babu lalacewa ko gurɓatawa
| ≥ 40 ng/µL 4 µg/kwayoyin kwarara/samfuri
1.7-2.2
≥1.5 | Hankali Adadin
OD260/280
OD260/230
Iyakance ko babu lalacewa ko gurɓatawa | ≥ 50 ng/µL 10 µg/kwayoyin kwarara/samfuri
1.7-2.2
1.8-2.5 |
| Shirye-shiryen Laburare marasa PCR: Hankali ≥ 40 ng/µL Adadin ≥ 500 ng | |||||
Gudun Aikin Sabis
Samfurin bayarwa
Cire DNA
Gina ɗakin karatu
Jeri
Binciken bayanai
Isar da bayanai
Ya haɗa da bincike mai zuwa:
- Raw Data Quality Control
- Ƙididdiga na daidaitawa zuwa tunani genome
- Bambance-bambancen ganewa: SNP, InDel, SV da CNV
- Bayanin aiki na bambance-bambancen karatu
Ƙididdiga na daidaitawa don yin la'akari da kwayoyin halitta - rarraba zurfin jeri
Kiran SNP tsakanin samfurori da yawa
Gano InDel - ƙididdiga na tsawon InDel a cikin yankin CDS da yanki mai faɗin genome
Bambance-bambancen rarraba a ko'ina cikin kwayoyin halitta - makircin Circos
Bayanin aiki na kwayoyin halitta tare da bambance-bambancen da aka gano - Gene Ontology
Chai, Q. et al. (2023) 'A glutathione S-transferase GhTT19 yana ƙayyade launin furen fure ta hanyar daidaita tarin anthocyanin a cikin auduga', Plant Biotechnology Journal, 21 (2), p. 433. doi: 10.1111/PBI.13965.
Cheng, H. et al. (2023) 'Chromosome-level daji Hevea brasiliensis genome yana ba da sabbin kayan aiki don taimakon kiwo da ƙima mai mahimmanci don haɓaka yawan amfanin gona', Plant Biotechnology Journal, 21(5), shafi 1058-1072. doi: 10.1111/PBI.14018.
Li, A. et al. (2021) 'Genome of the estuarine oyster yana ba da haske game da tasirin yanayi da filastik daidaitacce', Sadarwar Halittu 2021 4: 1, 4 (1), shafi 1-12. doi: 10.1038/s42003-021-02823-6.
Zeng, T. et al. (2022) 'Bincike na genome da methylation canje-canje a cikin kajin 'yan asalin kasar Sin a tsawon lokaci yana ba da haske game da kiyaye nau'in jinsin', Sadarwar Halittu, 5 (1), shafi 1-12. doi: 10.1038/s42003-022-03907-7.
