Shuka / dabba de novo na kwarai
De novoSearquincing yana nufin gina wani nau'in halittu na gaba daya ta amfani da fasahar sequencing a babu wani tunani mai zurfi. Gabatarwa da gabatarwar samfuri na uku, wanda ke bayyana mai tsawo yana karanta, yana da haɓaka taro mai yawa ta hanyar ƙara yawan maye a tsakanin karantawa. Wannan haɓaka yana da dacewa musamman lokacin da ake ma'amala da ƙalubalen ƙarar, kamar waɗanda ke nuna abubuwan da ke tattare da abubuwa marasa kyau, ko yankuna masu tsinkaye waɗanda ake magana da su ta amfani da sequencation shi kadai.
Magani na tsayawa na daya na baya yana samar da sabis ɗin da aka haɗa da bincike da bincike mai zurfi wanda ke ba da babban inganci-inganci wanda de Novo ya tattara jiki. Binciken na kwarai da gaske tare da rashin lafiya yana ba da kimantawa girman girman girman da rikice-rikice, kuma ana amfani da wannan bayanin don jagorantar bayanan da ke gaba tare da Pacbio Hifi na gaba tare da Pacbio HIFI,de NovoMajalisar Contigs. M amfani amfani da Majalisar dokar HILLS yana bawa anchors ga ci gaba, samun babban taron chromosome. A ƙarshe, na da gaske suna da tsinkayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar halittu, suna komawa zuwa rubutun da gajeru da dogon karantawa.
Abubuwan sabis
● Haɗin haɗawa da ayyuka masu yawa da kuma ayyukan ta'addanci a cikin mafita na tsayawa:
Binciken na gaske tare da rashin lafiya don kimanta girman girman da jagoranci na gaba;
Dogon karanta sequincing forde NovoMajalisar Contigs;
Hi-c sequin don chromosome angoring;
Mista Sequincing don ambaton kwayoyin halittar;
Inganta Majalisar.
Ari a sabis ɗin da ya dace da abubuwan da keɓaɓɓen nassi ko haɓaka abubuwan gonar da ke faruwa don nau'in sha'awar.
Fa'idodi na sabis
Haɓaka dandamali dandamali da kuma bintioinformatics inde Novotaro na gaske
(Amarasinghe sl et al.,Ilmin dabbobi na asali, 2020)
●Kyakkyawar ƙwarewa da littafin Buga: Bmkgene ya tara babban kwarewa a cikin babban taro mai inganci na nau'in nau'ikan daban-daban, gami da kyawawan nau'ikan kayan kwalliya da kuma nau'ikan nau'ikan kayan polyploid. Tun shekara ta 2018, mun ba da gudummawa ga sama300 manyan wallafe-tallace, da kuma 20+ daga cikinsu ana buga su a cikin yanayin halittar yanayi.
● Tsayar da tsira: Hanyar da aka haɗa mu ta haɗu da fasahohin da yawa da kuma masu bincike na bioinformat a cikin aikin motsa jiki, suna kawo cikakkiyar ingantacciya.
●Wanda aka daidaita shi da bukatunku: Aikinmu na sabis ɗinmu yana zama kamar karbuwa don abubuwan da ke tattare da fasali dabam-dabam da takamaiman bukatun bincike. Wannan ya hada da saukar da manyan kwayoyin, dabbobin polyploD, man heterygous kwayoyin halitta, da ƙari.
●Sosai 'yan kwararrun manufofin da kuma kungiyar dakin gwaje-gwaje: Tare da kwarewa mai girma a duka gwajin da kuma manufar gaba gaban majalissar mazaje da jerin gwano da haƙƙin mallaka.
●Tallafin Post-tallace-tallace:Jinbarmu ta wuce ta wuce matakin aiki tare da lokacin 3 bayan siyarwa. A wannan lokacin, muna bayar da fa'idar bi, don taimako na matsala, da kuma zaman don magance duk wasu tambayoyin da suka shafi sakamakon.
Bayani na Sabis
| Bincike na gudu | Taro na gaske | Matsayin-matakin | Alamar Rayuwa na Gan |
| 50x clicumina Novaseq pe150
| 30x pacbio ccs HIFI ya karanta | 100x hi-c | Rna-seq olumina pe150 10 GB + (Zabi) Cikakken Tsawon RNA-SEQ Pacbio 40 GB ko Nanopore 12 GB |
Bukatar Sabis
Don binciken bincike, Majalisar Majalisar Ta Hi-C
| Nama ko kuma fitar da acid na nucleic | Bincike na gudu | Taro na gaske tare da Pacbio | Hi-C Majalisar |
| Viscera ta dabbobi | 0.5-1 g
| ≥ 3.5 g | ≥2 g |
| Tsoka | ≥ 5 g | ||
| Jinyar Mammalia | 1.5 ml
| ≥ 5 ml | ≥2 ml |
| Kaji / jini kifi | ≥ 0.5 ml | ||
| Shuka- sabo ganye | 1-2 g | ≥ 5 g | ≥ 4 g |
| Sel mai sulhu |
| ≥ 1X108 | ≥ 1X107 |
| Ƙwaro | 0.5-1 g | 3 g | ≥ 2 g |
| An fitar da DNA | Taro: ≥1 NG / μL Adadin ≥ 30 ng Iyakance ko babu lalata ko gurbatawa | Tankali: ≥ 50 ng / μl Adadin: 10 μg / Rarara Cell / Samfura OD260 / 280 = 1.7-2.2 Od260 / 230 = 1.8-2.5 Iyakance ko babu lalata ko gurbatawa |
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Don kalmar annashuwa tare da fassarar fassarar:
| Nama ko kuma fitar da acid na nucleic | Tallafi na rashin lafiya | Tallafin Pacrio | Tallafin nanopore |
| Shuka- tushen / tushe / petal | 450 mg | 600 mg | |
| Shuka - ganye / Seed | 300 mg mg | 300 mg mg | |
| Shuka - 'Ya'yan itace | 1.2 g | 1.2 g | |
| Zuciyar dabba / hanji | 300 mg mg | 300 mg mg | |
| Dabbobin kwayar halitta / kwakwalwa | 240 mg | 240 mg | |
| Tsoka | 450 mg | 450 mg | |
| Kasusuwa na dabbobi / gashi / fata | 1 g | 1 g | |
| Arthropod - kwari | 6 | 6 | |
| Arthropod -crustace | 300 mg mg | 300 mg mg | |
| Gaba daya jini | 1 bututu | 1 bututu | |
| An fitar da RNA | Tankali: ≥ 20 ng / μl Adadin ≥ 0.3 μg OD260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Zewaye≥ 6 5ET28 / 18S≥1 | Tankali: ≥ 100 ng / μl Adadin ≥ 0.75 μg OD260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Zewaye≥ 8 5ET28 / 18S≥1 | Tankali: ≥ 100 ng / μl Adadin ≥ 0.75 μg OD260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Zewaye7.5 5ET28 / 18S≥1 |
Bayar da Shawara
Akwati: 2 ml centrifuge bututu (tin capil ba da shawarar)
(Ga yawancin samfurori, muna ba da shawarar kada a adana a Ethanol.)
Allon lakabin: samfurori dole ne a bayyane kuma daidai ga ƙaddamar da bayanin bayanan samfurin.
Jirgin ruwa: bushewa-kankara: samfurori bukatar a cushe a cikin jakunkuna da farko an binne shi a cikin bushe-kankara.
Wakoki
Aikin aikin sabis
Tsarin gwaji
Samfura
Hakar DNA
Kungiyar Kwadadi
Kazalala
Bincike na bayanai
Bayan Siyarwa
Cikakken bincike na Bioin normorgator, rabu a matakai 4:
1) Bincike na gudu, dangane da nazarin K-Mer na bincike tare da ngs karanta:
Kimanta girman girman
Kimantawa na heterozygnity
Kimanta yankuna masumaitawa
2) Majalisar Gentome tare da PACBOO HIFI:
De Novotaro
Gwajin taro
3) HI-C Majalisar:
Hi-C Library Qc: kimanta ingantattun Hi-C
Majalisar Hi-C
Hi-C Euraliation
4) Rayuwar Gasana:
Hasashen Non-Cheoding RNA
Maimaita jerin shaidar (transpoosons da Tandem ya maimaita)
Hasashen Gene
§De Novo: AB TAFIYA ALGORIHMS
§ tushen ilimin ikiti
§ An dogara da lasisi, tare da dogon lokaci da gajeren karanta: karanta sunede NovoTaro ko tsara shi zuwa daftarin gaske
§ faduwar halittu masu tarin yawa tare da bayanai da yawa
1) na kwayar halitta - bincike na K-da
2) Majalisar Gunduma
2) Majalisar Gailuwa - Pacbio HIFI tana karanta taswirar taswira zuwa Majalisar
2) Majalisar Hi-C - kimanta Hi-C ingantacciyar hulɗa da nau'i-nau'i
3) Hi-C Post-Majalisar Dokar
4) Rayuwar Rayuwa mai zurfi - Haɗin halittar annabta
4) Annotation na asali - kalmar annabta
Bincika cigaban da aka sauƙaƙe ta hanyar Bmkgene na Majalisar De Novo na Novo ta hanyar tarin littattafan da aka zage su:
Li, C. et al. (2021) 'jerin' yan ƙasa suna bayyana hanyoyin da ba su dace ba kuma suna ba da shawarar Convertarancin kwayoyin halittar a cikin Saturly Juyin Halitta ', Tattalin sadarwa, 12 (1). Doi: 10.1038 / s41467-021-21379-x.
Li, Y. et al. (2023) 'Manyan canje-canje na Chromosomal suna haifar da canji na ainihi, daidaitawa na muhalli, da kuma fannoni a cikin Gayal (Bos Frontalis)', ilimin ƙwayoyin cuta da juyin halitta, 40 (1). doi: 10.1093 / molbev / msad006.
Tian, T. et al. (2023) Majalisa na Gentome da kuma kwayoyin halittar sanannen shahararren fari-resistication masara gerpmplasm ', yanayin halittu 2023 55: 3, 55 (3), PP. 496-506, PP. 496-506, PP. 496-506. Doi: 10.1038 / s41588-01297-y.
Zhang, F. et al. (2023) 'Bayyanar da juyin halitta na alkootane na biosynthesis ta nazarin Genomes biyu a cikin dangin Solanaceae a cikin dangin Solanaceae, Yanayin Sadarwa 2023 14: 1, 14: 1, 14 (1), PP. 1-18. Doi: 10.1038 / s41467-023-37133-4.
Kalubalantar kararraki:
Telomere-to-Telomere:Fu, a. A. et al. (2023) 'Telomerey-zuwa-mai-hazo a cikin Delon mai ɗaci mai ɗorewa (Momorrate Chantio L. var. Abbrevia Sert.) Bayyana Haɓakar' Ya'yan itace ', Binciken Gasetic, 10 (1). Doi: 10.1093 / HR / UHAC228.
HAPLotype Ciki:Hu, W. et al. (2021) 'Alale-da aka ayyana Gwal ya bayyana da bambancin Biallleuwa lokacin Tassiva Juyin halitta', inji kwayoyin halitta, 14 (6), PP. 851-854. doi: 10.1016 / j.molp.2020..044.
Babban Majalisar Dinama:Yuan, J. et al. (2022) 'GASKIYA IN GIGA-Chromosomes da Giga-Irmiya na Iton Peony Peony Peony OSII, Yanayin sadarwa 2022 13: 1, 13 (1), PP. 1-16. doi: 10.1038 / s41467-025063-1.
PolyploD na Gasar Provel:Zhang, Q. et al. (2022) 'Gashin-kirkire'in da aka samu a cikin ragewar jakar jakar jakarwar kwanan nan don Saccharum Sponan', Tsarin halittar 2022 54: 6, 54: 64 (6), PP. 885-896. doi: 10.1038 / s41588-022-02084-1.
