Mrna-Seq (NSG) tare da na kwarai

Mrna-Seq (NSG) tare da na kwarai

Rna-Seq shine daidaitaccen kayan aiki a cikin rayuwa da ƙimar ƙimar gona, suna jujjuya rata tsakanin halittu da kariya. Styarfinta ya ta'allaka ne a cikin gano sabbin shafukan da qufita furucinsu a cikin assday. Ana amfani da shi sosai don karatun mai hankali, zubar da haske akan kwayoyin halittu masu alaƙa da halaye daban-daban ko bayyanar da bayyanar kwayoyin halitta a ƙarƙashin takamaiman yanayi. BMkClcoud mna (tunani) App yana haɗa da kalmar faɗi, ra'ayoyi daban-daban (deg), da kuma ambaliyar tsarin da ke cikin ƙarfi na Mrna-seq (ngs) da ƙarfi na Mrna-seq (ngs) da ƙarfi na software irin, tabbatar da dacewa da aboki-mai amfani. Masu amfani za su iya loda bayanan su RNA-SeQ game da gajimare, inda app ɗin yana ba da cikakkiyar fahimta, mafita-dakatarwa mai tsayayye na tantancewa. Ari ga haka, yana da kwarewar abokin ciniki, bayar da ayyukan keɓaɓɓen ayyukan da aka kera wa takamaiman bukatun masu amfani. Masu amfani na iya saita sigogi da ƙaddamar da opline manufa da kansu, duba bayanan / zane-zane da kuma kammala ma'adinai / kamar: gyaran riga, custering aiki iri, da sauransu, da sauransu, da sauransu, da sauransu.

Sakamakon demo

Bayanai

Bukata

Babban bincike

Takardar shaida

Sakamakon demo

Bayanai

Bukata

Dogara:Illumina, MGI
Dabarun:Rna-seq
Aikin shirya fuloti: Paried, mai tsabta-bayanai.
Nau'in laburare:Fr-unstraided, Fr-Farkon Ko FR-Securd
Karanta tsawon:150 BP
Nau'in fayil:*. * .fq, * .GUQ.GZ ko * .fq.gz. Tsarin zaita atomatik ya haɗa da fayilolin.misali * _1.faff ada da * ._ 2. 2.FikQ.
Yawan samfurori:Babu hani a kan lambarSamu samfurori, amma lokacin bincike zai kara yawan adadinsamfuran suna girma.
Adadin bayanan da aka ba da shawarar:6g kowane samfurin

Babban bincike

Babban kayan bincike da kayan aikin mrna-seq (nasihu)bututun mai kamar haka:

1. Ingancin ingancin Rawdata:

• Cire na daidaitawa da ƙananan tsari, jerin adaftar,sauransu;

• Kayan aiki: A cikin-gidan da aka samo bututun ruwa;

2. Juyin bayanai zuwa mai kyau na gaske:

• Daidaita karanta tare da Algorithm mai santsi a kanMagana na gaske.

• Kayan aiki:Herat2, Samtools

3. Mai ƙididdigar ɗakin karatu na laburare:

• Sanya nazarin tsinkaye, bincike mai cikakken tsari, da sauransu;

• Kayan aiki:Samtools;

4. Bincike Tsarin Tsarin:

• Madadin bincike na Spisicing, ingantawa na tantanin halitta,Magana kwayoyi, da sauransu;

• Kayan aiki:Kunnen karfe, gffcompare, Ant,Lu'u-lu'u, Interproscan, daAmmer.

5. Bambanci ra'ayi:

• Deg allon, bincike na haɗin gwiwa, aikin aikiwadatarwa;

•Sakamako daban-daban sakamakon;

•RdaSegseq, Deseq2, GGPLOT2, Dexseq

Takardar shaida

1. Kim, Dehwan et al. "Glayer-tushen jeri dagenaroy tare da herat2 da kuma nasa. "HaliIlmin kimiya37 (2019): 907 - 915.

2. Mckenna, Haruna et al. "Kayan aikin kayan aikin na kwarai: aTsarin Kasuwanci na Mapredick don Nazarin DNADaskararren data. "Binciken Bincike na Gaskiya20 9 9 9 (2010): 1297-303.

3. Li, Heng et al. "The jerin jeri / taswirci tsari daSamtools. "Bioinformatics25 (2009): 2078 - 2079.

4. Perţea, MIHAELA et al. "Strettie yana ba da damar ingantasake gina sabar daga RNA-SeQ ya karanta. "HaliIlmin kimiya33 (2015): 290-295.

5. Soyayya, Michael I. et al. "Matsakaicin kimantawa na ninkiDiscpeontion ga bayanan RNA-seq tare da Deseq2. "MIlmin dabbobi15 (2014): n. pag.

6. Eddy, Sean R .. "hanzarta bincike na Hmm."Plro Ilmin lissafi7 (2011): n. pag.

Mrna-Seq (NSG) tare da na kwarai

Mrna-Seq (NSG) tare da na kwarai

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