Ainihin juyin halitta
Pnas
Asalin juyin halitta da tarihin gida na kifin gwal (carassius auratus)
Pacbio | Illumina | Taswirar Bionano Gentome Hi-C na Gentome Majalisar | Taswirar Getitic | GWAI | Rna-seq
Karin bayanai
An sabunta Lafiya na 1.goldfish tare da babban taro mai inganci, an fassara shi kashi 95.75% na contigs cikin 50 pseudochromosomes (scaffold N50 = 31.84 MB). An gano subgenomes biyu.
2. An bayyana yankuna na qarshe na zaban a yayin zanga-zangar da aka gano daga kwace bayanan mutane na 2019, sun bayyana wasu halittun na 390 wadanda ake da alaƙa da halaye na gida.
3.Gwas akan finan zinari a cikin gidafi na zinare da aka bayyana cewa ya haifar da kwararrun masana'antu wanda zai iya yin alaƙa. An gano wani mai furotin-furotin Kinase a matsayin ɗan takarar causalen da ke hade da nuna gaskiya
Baya
Golarfish (Carassius Aurassatus) yana daya daga cikin mahimman kifi, wanda ke zaune daga kifin Cracia a tsohuwar. Charles Darwin ya sharhi a matsayin "wuce kusan bambancin launuka na launi, muna haduwa da mafi yawan gyare-gyare na tsari". Mafi mahimmancin fasali da dogon tarihin gida da kiwo suna sanya kifin zinari na tsarin halittar Gene na ilimin halittar jini don Kifi na Hishology da juyin halitta.
Samun nasarori
Goldfish na zinari
JBinciken acbio da bayanan da aka yi amfani da su na Pacbio da kuma bayanan da aka yi amfani da su na farko na 1.657 g daftarin taron (Contigaig N50 = 474 KB). An samo taswirar Optanop na Bionano kuma ya gyara taron jama'a a cikin 1.73 GB a girma (kimanta girman girman: 1.8 gb). Majalisar Hi-C dangane da sikelin Hi0 daga 606 kilomita 31.84 MB kuma an cimma shi 95.75% (1.65 GB) da anchored anching. Gwardan ya ƙunshi kayan kwalliyar halittar 56,251 da kuma rubutun baƙon rubutu mai tsayi. Haka kuma, an yi hasashen yankuna na 38 daga cikin chromosomes 50.
Fifi.1 Gold Kifi
TWo bayyane saiti na subgenomes an gano shi a cikin chromshan kifi 50 wanda ya haifar da tsohuwar abin da ya faru da aka yi. The set of chromosomes with higher proportion of reads aligned between goldfish and Barbinae were defined as subgenome A (ChrA01~A25), ie subgenome common to Barbinae, and the remainings as subgenome B (ChrB01~B25).
Gida da zabar seeps
AJimlar kwalliyar murhun 16 na daji da kuma magunguna na zinare 185 suna gudanar da bincike mai zurfi tare da zurfin zage-zangar kusan 12.5x, samar da 4.3 terabasantawa na bayanai. Binciken Phylogenetic da kuma binciken PCCA na tabbatar da dangantaka kusa da kifayen zinare da crucian crup fiye da sauran kifin gwal, wanda ƙarshen ya raba kashi biyu.
LBinciken D lalacewa akan abubuwan hawa hudu da aka tallafa wa wanzuwar mutane na halittun halittun kwayar halittu a lokacin gida da kuma zabin wucin gadi a cikin zinari. Aryara yawan nau'ikan halittar (π) daga kifin giwa na gama gari zuwa Wen Goldfish da ƙwararrun kifin bambancin ƙwayar cuta a cikin gida. 50 Zaɓaɓɓen fashewar yankuna masu tarin abubuwa suna rufe 25.2 MB da 946 kwayoyin halitta daga bayanan wakilai (33 gas na zinari 16 na giciye 16). Fadada nazarin zuwa ga 201 na mutane, kwayoyin halitta 393 sun nuna yankuna na zaɓaɓɓen zaɓaɓɓu. An samo waɗannan ƙananan halittar, waɗanda zasu iya ba da gudummawa ga abubuwan da ke da alaƙa da manyan halaye na gida a cikin kifin gwal.
Fig.3 na asali-da aka danganta
Gwas a kan kifin zinari
DFin finafinan orsal babban fasalin mabuɗin ya danganta wen gwal mai zinare daga kifin gwal kwai. Gwas na dorsal finni na 96 Wen Goldfish da 87 kwai gwanayen 'yan takarar da aka bayyana a fadin chromosomes 13 da kuma rashin daidaituwa rarraba tsakanin subgenomes an lura. Nazarin aiki akan waɗannan 'yan takarar halittar halittar halittu wadanda suka hada da "Selel Studentation", "Ci gaban Sirletal", da sauransu.
FIG.4 GWAS na Dorsal Fin akan kifin zinari
IN an gano cewa an gano wata ƙungiya mai ƙarfi guda ɗaya. An gano cewa an gano cewa an gano cewa an gano karar Tyrosine na Tyrosine na Tyrome a daya daga cikin sakilla.
FIG.5 GWas na halaye masu dangantaka mai rikitarwa
Takardar shaida
Chen d et al. Asalin juyin halitta da tarihin gida na kifin gwal (carassius auren). Pnas (2020)
Labaru Manufar da za a raba lokuta masu nasara tare da nazarin halittu na biomerker, kwace jaraba da aka kwato da kuma manyan dabarun da aka yi yayin binciken.
Lokaci: Jan-04-022