T2T na Gentome, ya ba da kyauta
1stAbubuwa biyu shinkafa1
Taken: Haɗin kai da Ingancin Gaggawa na Gasa guda biyu na Kasa
Doi:https://Doiorg/10.1101/2020.12.24.424073
Lokaci da aka sanya: 01, 2021.
Cibiyar Noma: Jami'ar Aikin Noma, China
Kayan
O. Sativa Xian / IndicaIri na shinkafa 'zhenshan 97 (zs97)' da 'minghui 63 (MH63)
Tsarin Sequining
NGS Karanta + Hifi ya karanta + CHR karanta + Bionano + Hi-C
Bayanai:
Zs97: 8.34 GB (~ 23x) HIFI karanta + 48.39 GB (~ 13x) CLR karanta + 25 GB (~ 69x) Ngs + 2 Bionano Iy Sels
MH63: 37.88 GB (~ 103x) HIFI ya karanta + 48.9x) CLR (~ 132x) CLR (~ 76x) clr 60 GB (~ 76x) Ngs + 2 Bionano Iy Sells
Hoto 1 Gaggawa Na Kyauta biyu na Rana (MH63 da Zs97)
2ndWasan na farko2
Taken: Telomere-da-Telomere Gaullessomes na banana ta amfani da Nanopore Sequincing
Doi:https://Doiorg/10.101/2021.040017
Lokacin da aka buga: Afrilu 17, 2021.
Cibiyar: Université Paris-Sclay, Faransa
Kayan
Double HaploidMusa AcuminataSPPMalacCeSis(Dh-pahang)
Dabarun sequinate dabarun da bayanai:
Yanayin Heneq2550 + miniion / Promethion (93GB, ~ 200X) + Taswirar Taswirar Ept (DLE-1 + BSPQ1)
Tebur 1 Kwatantawa Musa Acuminata (DH-Pahang) manyan majalissar
Hoto na 2 Masifa Tsarin Tsarin gine-ginen gine-gine
3rdPhieadactylum Tricornutum na asali3
Take: Telomere-to-Telomere Babban MajalisarP
haeodactylum tricornutum
Doi:https://Doiorg/10.1101/2021.05.044.442596
Lokacin da aka buga: Mayu 04, 2021
Cibiyar: Jami'ar yamma, Kanada
Kayan
Phieadactylum Tricornutum(Taladin Aljila na Algae da Protozoa Ccap 1055/1)
Dabarun sequinate dabarun da bayanai:
1 Jirgin saman Oxford Nanopore ya kwarara Cell + A 2 × 75 pire-karshen Tsakanin Sputseq 550 Run
Hoto na 3 Ganewa don Majalisar Dinkin Duniya ta Telomere
4thDan Adam Chm13 na farko4
Title: Cikakken jerin 'yan Adam
Doi:https://Doi.org/10.1101/2021.05.445798
Lokacin da aka buga: Mayu 27, 2021
Cibiyar: Cibiyoyin Kiwon lafiya (NIH), Amurka
Kayan aiki: Layi na sel CHM13
Dabarun sequinate dabarun da bayanai:
30 × PACBIO Madaukakakun yarjejeniya (HIFI), 120 × Exford-Free Sequincing (1000 × Illumin / Arima Gentomics PCR-Free Sequincing (Haima Genomics PCR -, da Strand-Seq
Table 2 Kwatanta Grch38 da T2t-CHM13 Majalisar mutane na mutane
Takardar shaida
1.sgereny nurk et al. Cikakken jerin 'yan Adam. borxiv 2021.05.26.445798; Doi:https://Doi.org/10.1101/2021.05.445798
2.3Jaroline Saller et al. Telomere-to-Telomere Gaintosomes na banana ta amfani da Nanopore Sequincing. borxiv 2021.04.16.440017; Doi:https://Doiorg/10.101/2021.040017
3.Daniel J. gigie et al. Telomere-to-Telomere na Ganuwa na Phiedaactylum Tricornutum. Borxxiv 2021.05.05.05.096; Doi:https://Doiorg/10.1101/2021.05.044.442596
4.jia-ming song et al. Majalisar da Ingancin Garkunan Kyauta guda biyu na Kasa don shinkafa Xian / Indica ta nuna kyakkyawar fahimta cikin gine-gine na centrometore. borxiv 2020.12.24.424073; Doi:https://Doiorg/10.1101/2020.12.24.424073
Lokaci: Jan-06-022