mRNA-seq (NGS) - De novo

Tsarin mRNA yana ba da damar bayyana duk kwafin mRNA a cikin sel a ƙarƙashin takamaiman yanayi kuma fasaha ce da ake amfani da ita sosai a wuraren bincike daban-daban. Bututun BMKCloud De novo mRNA-seq an ƙera shi don nazarin ɗakunan karatu na haɓakar poly-A lokacin da babu kwayar halitta. Bututun yana farawa da kula da inganci, sannan kumada novotaron kwafi da zaɓin saitin unigene. Binciken tsarin unigene yana annabta jeri na coding (CDS) da sauƙaƙan maimaita maimaitawa (SSR). Bayan haka, ƙididdigar magana ta bambanta ta sami nau'ikan da aka bayyana daban-daban (DEGs) a cikin yanayin da aka gwada, sannan bayanan aiki da haɓaka DEGs don fitar da bayanan ilimin halitta.