Hi-C Based Genome Assembly
Hi-C wata hanya ce da aka ƙera don ɗaukar daidaitawar chromosome ta hanyar haɗa ma'amala ta tushen kusanci da babban tsarin aiwatarwa. An yi imanin ƙarfin waɗannan hulɗar yana da alaƙa mara kyau tare da nisa ta jiki akan chromosomes. Don haka, ana amfani da bayanan Hi-C don jagorar tari, oda, da daidaita jerin abubuwan da aka taru a cikin daftarin kwayoyin halitta da kuma rataya waɗancan kan takamaiman adadin chromosomes. Wannan fasaha tana ba da ƙarfin taro na matakin chromosome a cikin rashin taswirar tushen yawan jama'a. Kowane genome guda ɗaya yana buƙatar Hi-C.
Siffofin Sabis
● Sequencing akan Illumina NovaSeq tare da PE150.
Sabis yana buƙatar samfuran nama, maimakon acid ɗin nucleic da aka fitar, don ƙetare haɗin gwiwa tare da formaldehyde da kiyaye hulɗar DNA-protein.
● Gwajin Hi-C ya haɗa da ƙuntatawa da kuma ƙarshen gyaran ƙarshen manne tare da biotin, sannan ta hanyar zagayawa na sakamakon da ba daidai ba yayin kiyaye hulɗar. Ana cire DNA ɗin tare da beads na streptavidin kuma a tsarkake su don shirye-shiryen ɗakin karatu na gaba.
Amfanin Sabis
Rahoton da aka ƙayyade na Hi-C
(Lieberman-Aiden E et al.,Kimiyya, 2009)
●Kawar da Bukatar Bayanan Al'ummar Halitta:Hi-C yana musanya mahimman bayanan da ake buƙata don ci gaba da tsayawa.
●Maɗaukakin alamar alama:yana haifar da haɓakar haɓaka mai girma sama da 90%.
●Ƙwararrun Ƙwararru da Bayanan wallafe-wallafe:BMKGene yana da kwarewa mai yawa tare da fiye da 2000 lokuta na Hi-C Genome Assembly daga nau'ikan nau'ikan nau'ikan 1000 da wasu haƙƙin mallaka. Fiye da shari'o'in 200 da aka buga suna da tasirin tasiri sama da 2000.
●ƙwararrun ƙungiyar bioinformatics:tare da haƙƙin mallaka na cikin gida da haƙƙin mallaka na software don gwaje-gwajen Hi-C da nazarin bayanai, haɓakar software na gani na gani da kansa yana ba da damar toshe motsi na hannu, juyawa, sokewa, da sake yi.
●Tallafin Bayan-tallace-tallace:Alƙawarinmu ya wuce bayan kammala aikin tare da lokacin sabis na watanni 3 bayan-sayar. A wannan lokacin, muna ba da bibiyar aikin, taimako na warware matsala, da kuma zaman Q&A don magance duk wata tambaya da ta shafi sakamakon.
●Cikakken Bayani: muna amfani da ma'ajin bayanai masu yawa don yin aikin tantance kwayoyin halitta tare da bambance-bambancen da aka gano da kuma yin nazarin haɓaka mai dacewa, samar da basira akan ayyukan bincike da yawa.
Ƙayyadaddun Sabis
| Shirye-shiryen ɗakin karatu | Dabarun jeri | Fitowar bayanai da aka ba da shawarar | Kula da inganci |
| Hi-C library | Illumina NovaSeq PE150 | 100x | Q30 ≥ 85% |
Samfuran Bukatun
| Nama | Adadin da ake buƙata |
| Animal Viscera | 2 g ku |
| tsokar dabba | |
| Jinin Mammaliyan | ≥ 2 ml |
| Jinin Kaji/Kifi | |
| Shuka- Fresh Leaf | 3g ku |
| Kwayoyin Al'ada | 1 x107 |
| Kwari | 2 g ku |
Gudun Aikin Sabis
Gwajin ƙira
Samfurin bayarwa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
1) Raw data QC
2) Hi-C laburare QC: kimanta ingantaccen hulɗar Hi-C
3) Hi-C taro: tari na contigs a cikin ƙungiyoyi, bi da contig oda a cikin kowane rukuni da kuma sanya contig fuskantarwa.
4) Hi-C kimantawa
Hi-C Library QC - kimanta Hi-C ingantattun ma'amala nau'i-nau'i
Hi-C Majalisar - ƙididdiga
Ƙimar bayan taro - taswirar zafi na ƙarfin sigina tsakanin bins
Bincika ci gaban da sabis na taron Hi-C na BMKGene ya sauƙaƙe ta hanyar tarin wallafe-wallafe.
Tian, T. et al. (2023) 'Taron kwayoyin halitta da rarraba kwayoyin halitta na fitaccen kwayar cutar masara da ke jure fari', Nature Genetics 2023 55:3, 55(3), shafi 496-506. doi: 10.1038/s41588-023-01297-y.
Wang, ZL et al. (2020) 'Taro mai-Chromosome-Scale of the Asian Honeybee Apis cerana Genome', Frontiers in Genetics, 11, p. 524140. doi: 10.3389/FGENE.2020.00279/BIBTEX.
Zhang, F. et al. (2023) 'Bayyana juyin halitta na tropane alkaloid biosynthesis ta hanyar nazarin kwayoyin halitta guda biyu a cikin dangin Solanaceae', Nature Communications 2023 14: 1, 14 (1), shafi 1-18. doi: 10.1038/s41467-023-37133-4.
Zhang, X. et al. (2020) 'Gomemes na Bishiyar Banyan da Pollinator Wasp suna ba da haske game da juyin juya halin Fig-Wasp', Cell, 183(4), shafi 875-889.e17. doi: 10.1016/J.CELL.2020.09.043
