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Hi-C tushen hulɗar Chromatin

Ma'anar Hi-C da aka Haɓaka Hoton hulɗar Chromatin

Hi-C wata hanya ce da aka ƙera don ɗaukar daidaitawar kwayoyin halitta ta hanyar haɗa ma'amala ta tushen kusanci da babban tsarin aiwatarwa. Hanyar ta dogara ne akan chromatin crosslinking tare da formaldehyde, biye da narkewa da sake sakewa ta hanyar da kawai gutsuttsura waɗanda ke da alaƙa da haɗin gwiwa zasu samar da samfuran ligation. Ta hanyar tsara waɗannan samfuran ligation, yana yiwuwa a yi nazarin ƙungiyar 3D na genome. Hi-C yana ba da damar nazarin rarraba sassan kwayoyin halittar da aka cika da sauƙi (A compartments, euchromatin) kuma mafi kusantar yin aiki a rubuce, da kuma yankunan da suka fi cunkoso (B compartments, Heterochromatin). Hakanan ana iya amfani da Hi-C don nunawa Topologically Associated Domains (TADs), yankuna na genome waɗanda suka naɗe da tsarin kuma suna iya samun nau'ikan maganganu iri ɗaya, da kuma gano madaukai na chromatin, yankuna DNA waɗanda sunadaran sunada alaƙa da su kuma waɗanda suke. sau da yawa wadatar da abubuwa masu tsari. Sabis ɗin jerin abubuwan Hi-C na BMKGene yana ba masu bincike damar bincika sararin sararin samaniya na genomics, buɗe sabbin hanyoyi don fahimtar ƙa'idodin kwayoyin halitta da tasirinsa a cikin lafiya da cuta.

Tuntube Mu

Cikakkun Sabis

Bioinformatics

Sakamakon demo

Fitattun wallafe-wallafe

Siffofin Sabis

● Sequencing akan Illumina NovaSeq tare da PE150.

Sabis yana buƙatar samfuran nama, maimakon acid ɗin nucleic da aka fitar, don ƙetare haɗin gwiwa tare da formaldehyde da kiyaye hulɗar DNA-protein.

● Gwajin Hi-C ya haɗa da ƙuntatawa da kuma ƙarshen gyaran ƙarshen manne tare da biotin, sannan ta hanyar zagayawa na sakamakon da ba daidai ba yayin kiyaye hulɗar. Ana cire DNA ɗin tare da beads na streptavidin kuma a tsarkake su don shirye-shiryen ɗakin karatu na gaba.

Amfanin Sabis

●Mafi kyawun Ƙuntataccen Enzyme Design: don tabbatar da ingantaccen Hi-C akan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan ma'amala masu inganci har zuwa 93%.

●Ƙwararrun Ƙwararru da Bayanan Bugawa:BMKGene yana da gogewa mai yawa tare da> 2000 Hi-C jerin ayyukan daga nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan 800 da haƙƙin mallaka daban-daban. Fiye da shari'o'i 100 da aka buga tare da tasirin tasiri sama da 900.

●Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru na Bioinformatics:tare da haƙƙin mallaka na cikin gida da haƙƙin mallaka na software don gwaje-gwajen Hi-C da nazarin bayanai da software na gani na gani da kansa.

●Tallafin Bayan-tallace-tallace:Alƙawarinmu ya wuce bayan kammala aikin tare da lokacin sabis na watanni 3 bayan-sayar. A wannan lokacin, muna ba da bibiyar aikin, taimako na warware matsala, da kuma zaman Q&A don magance duk wata tambaya da ta shafi sakamakon.

●Cikakken Bayani: muna amfani da ma'ajin bayanai masu yawa don yin aikin tantance kwayoyin halitta tare da bambance-bambancen da aka gano da kuma yin nazarin haɓaka mai dacewa, samar da basira akan ayyukan bincike da yawa.

Ƙayyadaddun Sabis

Laburare

Dabarun Jeri

Fitowar bayanai da aka ba da shawarar

Ƙaddamar Siginar Hi-C

Hi-C library

Farashin PE150

Chromatin madauki: 150x

KU: 50x

Madaidaicin Chromatin: 10Kb

Saukewa: 40KB

Bukatun Sabis

Nau'in samfurin	Adadin da ake buƙata
Naman dabba	≥2g ku
Dukan Jini	≥2 ml
Fungi	≥1g ku
Shuka- matasa nama	1g/aliquot, 2-4 aliquotes shawarar
Kwayoyin Al'ada	1 x10⁷

Na baya: TGuide Smart Magnetic Shuka RNA Kit

Na gaba: DNBSEQ dakunan karatu da aka riga aka yi

Ya haɗa da Binciken Mai zuwa:

● Raw bayanai QC;

● Taswirar taswira da ɗakin karatu na Hi-C QC: ingantattun nau'i-nau'i na hulɗar hulɗa da Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwaƙwalwa na Ƙaƙwalwa na Ƙaƙƙa ) na Ƙarfafawa ;

● Cikakkun hulɗa da ƙasa-ƙasa: CIS / trans na bincike da taswirar taɗi;

● Binciken rarraba A / B;

● Gano TADs da madaukai na chromatin;

● Bincike daban-daban akan abubuwan tsarin 3D na chromatin tsakanin samfurori da kuma bayanin aikin da ya dace na kwayoyin halitta.

Cis da rarraba rabon rabon

Taswirar zafi na hulɗar chromosomal tsakanin samfurori

Rarrabuwar sassan A/B mai faɗi

Genom-fadi rarraba madaukai chromatin

Kallon TADs

Bincika ci gaban binciken da sabis na jerin hi-C na BMKGene ya sauƙaƙe ta hanyar tarin wallafe-wallafe.

Meng, T. et al. (2021) 'Kwantanci hadedde multi-omics bincike ya gano CA2 a matsayin sabon manufa don chordoma',Neuro-Oncology, 23 (10), shafi na 1709-1722. doi: 10.1093/NEUONC/NOAB156.

Xu, L. et al. (2021) 'Rashin tsari na 3D da sake tsara kwayoyin halitta suna ba da haske game da pathogenesis na NAFLD ta hanyar haɗakarwar Hi-C, Nanopore, da RNA',Acta Pharmaceutica Sinica B, 11 (10), shafi na 3150-3164. doi: 10.1016/J.APSB.2021.03.022.