De novo fungal na ainihi taro
Bmkgene yana ba da mafi kyawun hanyoyin haɓaka na fungal, na dafa abinci daban-daban bukatun kuma ga nufin cikar jiki. Yin amfani da gajeren karanta ƙarancin karanta-karanta kawai ya ba da damar ƙarni na mai ɗorewa. Short-karantawa da dogon lokaci sequencing ta amfani da Nanopore ko Pacbio an haɗa su don ƙarin tsayayyen fungal tare da karin kwalliya. Haka kuma, hada kai Hi-C Sequinarin inganta karfin, yana ba da damar cimma cikakken ɗabi'ar da aka yi amfani da ita.
Abubuwan sabis
Tare da zaɓuɓɓukan da za a zaɓi uku don zaɓi daga gwargwadon matsayin da ake so na cikar Genome:
Zaɓin zaɓi na ƙarshe: Sixici na ɗan gajeren magana tare da rashin lafiya mara kyau a cikin NOVEQ pe150.
Oparancin Fright Oben-Gansome:
Binciken na Ganuwa: Ilymumina Novaseq PE150.
Majalisar na Ganuwa: Pacbio Revis (Hifio yana karantawa) ko Nanopore Promethion 48.
Ganyen Fungo
Binciken na Ganuwa: Ilymumina Novaseq PE150.
Majalisar na Ganuwa: Pacbio Revis (Hifio yana karantawa) ko Nanopore Promethion 48.
Contangar da aka tattara tare da Majalisar Hi-C.
Fa'idodi na sabis
●Dabarun jerin abubuwa da yawa: Ga burin bincike daban-daban da buƙatun cikakan gaba
●Kammala Bieriinformatics Work:Wannan ya hada da Majalisar Genome da Hasashen da yawa na abubuwa da yawa na asali, jigon kayan aikin, da kuma contigig criguring.
●Mai yawan ƙwarewa: Da sama da mahimman abubuwan ƙwayoyin cuta 12,000 suka taru, muna kawo shekaru goma na ƙwarewar bincike, babban abin da aka bincika, da kuma cikakkiyar goyon baya.
●Tallafin Post-tallace-tallace:Jinbarmu ta wuce ta wuce matakin aiki tare da lokacin 3 bayan siyarwa. A wannan lokacin, muna bayar da fa'idar bi, don taimako na matsala, da kuma zaman don magance duk wasu tambayoyin da suka shafi sakamakon.
Bayani na Sabis
| Hidima | Tsarin Sequining | Iko mai inganci |
| Mai tsara halittar | Illumina Pe150 100x | Q30≥85% |
| Mai kyau | Bincike na Ganowa: Rashin lafiya Pe150 50 x Taro: Pacbio HIFI 30X ko Nanopore 100x | Contig N50 ≥1MB (Pacbio Unicellular) Contig N50 ≥2M (ont Unicellular) Contangar N50 ≥500kb (wasu) |
| Chromosome-Stot | Bincike na Ganowa: Rashin lafiya Pe150 50 x Taro: Pacbio HIFI 30X ko Nanopore 100x Hi-C Majalisar 100x | Contangararrawa mai zane-zane> 90%
|
Buƙatar sabis
| Taro (NG / μL) | Jimlar adadin (μg) | Girma (μl) | Od260 / 280 | Od260 / 230 | |
| Pacbio | ≥20 | ≥2 | ≥20 | 1.7-2.2 | ≥1.6 |
| Nanopore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 2.0-3.0 |
| M | ≥1 | ≥0.06 | ≥20 | - | - |
Unicellular naman gwari: ≥3.5X1010 sel
Macro naman gwari: ≥10 g
Aikin aikin sabis
Samfura
Kungiyar Kwadadi
Kazalala
Bincike na bayanai
Bayan Siyarwa
Ya hada da bincike mai zuwa:
Binciken na Ganiya:
- Kulawa da ingancin ingancin bayanai
- Adalci na asali: Girma, heterozygnosn, maimaitawa
Kyakkyawan Majalisar Gastome:
- Kulawa da ingancin ingancin bayanai
- De NovoTaro
- Binciken Mahalicce: Hasashen CDs da Abubuwa da yawa
- Bayani mai aiki tare da yawancin bayanan gaba ɗaya (je, Kogg, da sauransu) da ci gaba da adana bayanai (katin, VFDB, da sauransu)
HI-C Majalisar:
- Hi-Cari na kimantawa.
- Clifiges achurer cluster, odar da kuma daidaituwa
- Kimanin taron majalisar Hi-C-C - dangane da ingantaccen taswirar zafi da zafi
Bincike na Ganiya: Ragrance Ragin K-Makariya
Babban taro na Gentome: An ambaton Gene
Majalisar Dinara: Annotation Gwardar Gentome (Go)
Bincika ayyukan ci gaba da sabis na Majalisar Dinkin Duniya na Bmkgal ta hanyar binciken tarin wallafen wallafen.
Hao, J. et al. (2023) 'Haɗa Omic mai zurfi na maganin naman kaza Indonotus Oblis Oblis a ƙarƙashin yanayin damuwa',Ƙwayoyin BMC, 24 (1), PP. 1-12. Doi: 10.1186 / s12864 (108864-0_956-z / lambobi / 3.
Lu, l. et al. (2023) 'Searquining na zuriya sun nuna juyin halitta da hanyoyin daukar ciki na alkama mai kaifi da karfi pathogen Rhizotonia Parkonia,Jaridar amfanin gona, 11 (2), PP. 405-416. Doi: 10.1016 / j.cj.2020.02.024.
Zhang, H. et al. (2023) 'Albarkatu na gaske don jinsin na Clarieeies Hissedies da ke haifar da tabo na dala cikin turbgress',Cutar shuka, 107 (3), PP. 929-934. Doi: 10.1094 / Pdis-08-22-1921-A
Zhang, s et al. (2023) 'Kayan Gashi da Shaida na Tsarin Tetrackolar na Tsarin Tetrackar a cikin Grifola Grifola Frondosa Frondosa',Jaridar Fungi, 9 (10), p. 959. Doi: 10.3390 / jof910099 / s1.
