Cikakken mrna sequecting-nanopore
Fasas
● Kulawa da Poly-Mrna ta biyo bayan Cdna kira da kuma shirye-shiryen laburare
● Rubuta na cikakken bayanan sakamako
Binciken Binciken Binciko wanda ya danganta ne da jeri na kwayar halitta
Binciken bincike na Bioini ya hada da ba kawai magana ba ne kawai a matakin Gene kuma amma kuma nazarin na LCRRNA, Gashin Gene, poly-adonylation da tsarin kwayoyin
Fa'idodi na sabis
●Daidaifi na magana a matakin neform mataki: Enagling cikakken bayani da cikakken bayani na tantancewa, canjin da aka bayyana wanda zai iya zama masked lokacin da nazarin dukkan furannin Gene
●Rage buƙatun bayanai:Idan aka kwatanta da na gaba-tsara na gaba (NGG), Nanoope Sequincing Nunin ƙananan buƙatun bayanai, ƙyale matakan daidai matakan bayyananniyar daraja jikewa tare da karami.
●Mafi girman daidaito na faɗi: duka a cikin kwayar halitta da kuma matakin gaske
●Shaida ƙarin ƙarin bayanin fassarar Transcriptomic: madadin polyadenyy, polyadenyalation, karin magana da lcnrna da kwayoyin halittunsu
●Mai yawan ƙwarewa: Teamungiyar mu tana kawo kwarewa ga kowane aiki, bayan da aka kammala sama da mutane 850 na nanopore cikakken bayanan ayyukan da aka sarrafa akan samfurori 8,000.
●Tallafin Bayanan Wasanni: Jagorarmu ta shimfida bayan kammala aikin tare da lokacin 3 bayan sayar da sabis. A wannan lokacin, muna bayar da fa'idar bi, don taimako na matsala, da kuma zaman don magance duk wasu tambayoyin da suka shafi sakamakon.
Bukatar samfuri da isarwa
| Leburare | Tsarin Sequining | Bayanai da shawarar | Iko mai inganci |
| Poly wani yanki | Illumina Pe150 | 6/12 GB | Matsakaicin maki mai inganci: Q10 |
Bukatun Samfura:
Nucheotides:
| Conc. (NG / μL) | Adadin (μg) | M | Kirki |
| 100 | 1.0 | OD260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Iyakantacce ko babu furotin ko gurbataccen DNA wanda aka nuna akan gel. | Don tsire-tsire: Rinto7.0; Ga dabbobi: Rinto7.5; 5.0≥28s / 18S≥1.0; iyakantacce ko babu wani yanki |
● tsire-tsire:
Tushen, kara ko petal: 450 mg
Ganye ko iri: 300 mg
'Ya'yan itace: 1.2 g
● dabba:
Zuciya ko hanji: 300 mg
Viscera ko kwakwalwa: 240 mg
Tsoka: 450 mg
Kasusuwa, gashi ko fata: 1g
● arthropods:
Kwari: 6g
Crustacea: 300 mg
Laifi gaba daya: 1 bututu
Kwayoyin sel: 106 sel
Bayar da Shawara
Akwati: 2 ml centrifuge bututu (tin capil ba da shawarar)
Allalan Samfura: Group + ya dace da misali a1, A2, A3; B1, B2, B3.
Jirgin ruwa:
1. Bushe-kankara: samfures bukatar a cushe a cikin jaka da binne a bushe-kankara.
2. Tumbunan rniastable: Ana iya bushewa sasƙarin RNA a cikin bututun RNA, og rnastable®) kuma an shigo cikin zazzabi a ɗakin.
Aikin aikin sabis
Nucheotides:
Samfura
Kungiyar Kwadadi
Kazalala
Bincike na bayanai
Bayan Siyarwa
Aikin aikin sabis
Fure:
Tsarin gwaji
Samfura
Hakar RNA
Kungiyar Kwadadi
Kazalala
Bincike na bayanai
Bayan Siyarwa
Processungiyoyin sarrafa bayanai
Gano gano rubutun
● madadin spclicing
● Uptaukar lamba a matakin kwayar halitta da matakin gaske
Musanya daban-daban na bincike
● Tunani na aiki da wadatarwa (robe da dets)
Madadin bincike na bincike
Mafarkin Polyadenymani na Polyadenyis (Apa)
lcranna hasashen
Annotation na kwayoyin halitta
Canjin dets
Kayan aikin jakadancin-furotin a cikin Dogs
Bincika cigaban abubuwa na bmkgene a tsakanin ayyukan Miskgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene a tsakanin ayyukan Miskgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na Mrkgene na MrKGene na MrKKDE cikakken aiki na MrKKGene na MrKGENCE Cikakke a tsakanin ayyukan Misira
Gong, B. et al. (2023) 'EpIGETTIL da kunna kunnawa na asirce Fari, da Oncogene da Generics da Generics da Generics da Generics da Generics, 50 (6), PP. 422-433. Doi: 10.1016 / j.jgg.20.0.008.
Shi, Z. et al. (2023) 'Cikakken rubutun asirin hymphocytes na lymphocytes amsa IF1 ta bayyana amsar rigakafi ta Th1, Olichthys Olivaceus)', 134, p. 108636. Doi: 10.1016 / j.fsi.023.108636.
Ma, Y. et al. (2023) na bincike na Pacbio da Ont RNAT RNA na biyu don nemopilema Nomurai Venom., Genomics, 115 (6), PA. 110709. Doi: 10.1016 / j.ygeno.2023.10709.
Yu, D. et al. (2023) 'Nazarin Nano-SeQ ya nuna bambanci daban-daban tsakanin ɓoyayyiya da kuma bambance-bambancen sel da aka samu daga Humsc', bincike sel da magani, 14 (1), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13), PP. 1-13). Doi: 10.1186 / s13287-023-03491-5 / Tables / 6.
