TGuide Smart Magnetic Shuka RNA Kit
Lambar kasida / marufi
| Cat. a'a | ID | No na preps |
| 4993552 (harsashi) | Saukewa: DP662-DE | 48 |
Jagoran zaɓi na Reagent don TGuide S16
| Nuclei acid | Nau'in Misali | Sunan samfur | Girman shiryarwa (preps) Harsashi / farantin karfe | Cat no. Harsashi / faranti |
| DNA | Naman dabba | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Shuka & iri | TGuide Smart Magnetic Shuka DNA Kit | 48 | 4993548 |
| DNA | Kasa/Stool | TGuide Smart Soil / Kit ɗin DNA | 48 | 4993549 |
| DNA | Gel & PCR samfurori | TGuide Smart DNA Tsabtace Kit | 48 | 4993550 |
| DNA | Dukan Jini | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Busashen Tabo, da sauransu | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Jini / Kwayoyin / Nama | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Shuka & iri | TGuide Smart Magnetic Shuka RNA Kit | 48 | 4993552 |
| DNA/RNA | Ruwan Jiki Kyauta (Serum, etc) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Gabatarwar samfur
Tsarkake yawan amfanin ƙasa, tsafta mai ƙarfi, jimillar RNA mai inganci daga jini/kwayoyin halitta/nama.
Babu wani abu mai guba kamar phenol/chloroform da ke ƙunshe
RNA da aka samu yana da tsafta mai girma kuma ana iya amfani dashi kai tsaye don gano guntu, babban tsari da sauran gwaje-gwaje.
Siffofin
Super sauki don amfani
Babu ƙarin aikin bututu.
Za a iya daidaita ƙarar haɓakawa kamar yadda ake buƙata.
Shigarwa da aiki yana buƙatar ƙaramin horo. Tare da shirye-shiryen da aka riga aka shigar, cire fakitin, zaɓi yarjejeniya kuma gudanar da gwajin ku. Shirye-shiryen suna shirye don amfani, kuma ana iya daidaita su.
An riga an ɗora reagents da madaidaitan abubuwan da za a iya zubarwa.
Ana kawar da sarrafa sinadarai zuwa mafi girma don tabbatar da amincin aiki.
Sakamakon Demo
Tushen alkama jimillar hakar RNA
Girman samfurin: 100 MG
Samfurin pretreatment: nika tare da ruwa nitrogen ko ƙananan zafin jiki nama homogenizer magani
Agarose gel maida hankali: 2% (TAE)
Loading girma: 2 μl
Alama: Alamar IV, TIANGEN
Sakamakon gwaji: ta amfani da TGuide S16 da aka samu tare da reagent 4993552 don cire jimillar RNA na tushen alkama ta atomatik yana da yawan amfanin ƙasa da tsabta. A cikin wannan gwaji, an fitar da kusan 20 μg na nucleic acid daga tushen alkama na 100 MG, tare da OD260 / OD280 a kusa da 2.0 ~ 2.1, da OD260 / OD230＞2.0.
Jimlar hakar RNA daga samfuran tsirrai daban-daban
Girman samfurin: 100 MG
Samfurin pretreatment: low-zazzabi homogenizer
Agarose gel maida hankali: 1% (TAE)
Ƙarar lodi: 1 μl
M: Alamar III, TIANGEN
1-4: tsaba na masara 5-8: ganyen masara 9-12: Tushen kabeji 13-16: waken soya
Samfurin biyu na farko an fitar da su ta hanyar kayan cire ginshiƙi kuma samfuran biyu na ƙarshe an fitar da su ta hanyar TGuide S16.
Sakamakon gwaji: yawan hakowar shuka RNA ta TGuide S16 tare da reagent 4993552 daidai yake da ko mafi kyau fiye da wancan ta kayan aikin cire ginshiƙi. Tsarkakewa da mutunci iri ɗaya ne. Don haka, ana iya amfani da TGuide S16 don fitar da jimillar RNA don maye gurbin maganin cirewar tushen ginshiƙi.
