TGuide Smart Blood/Cell/Tissue RNA Kit
Lambar kasida / marufi
| Cat. a'a | ID | No na preps |
| 4995043 (faranti) | DP661-E | 96 |
| 4993551 (harsashi) | Saukewa: DP661-DE | 48 |
Jagoran zaɓi na Reagent don TGuide S16
| Nuclei acid | Nau'in Misali | Sunan samfur | Girman shiryarwa (preps) Harsashi / farantin karfe | Cat no. Harsashi / faranti |
| DNA | Naman dabba | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Shuka & iri | TGuide Smart Magnetic Shuka DNA Kit | 48 | 4993548 |
| DNA | Kasa/Stool | TGuide Smart Soil / Kit ɗin DNA | 48 | 4993549 |
| DNA | Gel & PCR samfurori | TGuide Smart DNA Tsabtace Kit | 48 | 4993550 |
| DNA | Dukan Jini | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Busashen Tabo, da sauransu | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Jini / Kwayoyin / Nama | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Shuka & iri | TGuide Smart Magnetic Shuka RNA Kit | 48 | 4993552 |
| DNA/RNA | Ruwan Jiki Kyauta (Serum, etc) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Gabatarwar samfur
Tsarkake yawan amfanin ƙasa, tsafta mai ƙarfi, jimillar RNA mai inganci daga jini/kwayoyin halitta/nama.
Babu wani abu mai guba kamar phenol/chloroform da ke ƙunshe
RNA da aka samu yana da tsafta mai girma kuma ana iya amfani dashi kai tsaye don gano guntu, babban tsari da sauran gwaje-gwaje.
Siffofin
Super sauki don amfani
Babu ƙarin aikin bututu.
Za a iya daidaita ƙarar haɓakawa kamar yadda ake buƙata.
Shigarwa da aiki yana buƙatar ƙaramin horo. Tare da shirye-shiryen da aka riga aka shigar, cire fakitin, zaɓi yarjejeniya kuma gudanar da gwajin ku. Shirye-shiryen suna shirye don amfani, kuma ana iya daidaita su.
An riga an ɗora reagents da madaidaitan abubuwan da za a iya zubarwa.
Ana kawar da sarrafa sinadarai zuwa mafi girma don tabbatar da amincin aiki.
Sakamakon Demo
An yi amfani da Agilent 2100 don gano gaskiya.
Samfurin tushe: CD-1 mice
Matsayin samfurin: sabon jini duka
Girman samfurin: 200 μl
Sakamakon gwaji: jimillar RNA da aka fitar daga jini ta TGuide S16 sanye take da reagent 4993551 yana da yawan amfanin ƙasa da tsafta, kuma yana iya biyan buƙatun ƙasa.
high-throughput jerin.
Samfurin tushe: CD-1 mice
Ƙarar lodi: 1 μl. An narkar da hanta sau 5 kuma an loda shi.
Agarose gel maida hankali: 1%. Electrophoresis na 20 min a 6 V / cm;
M: TIANGEN Alamar III 1-4: zuciya 5-8:
hanta 9-12: huhu 13-16: koda
Samfurori biyu na farko na kowane nama an ciro su ta hanyar kayan cirewar tushen ginshiƙi, kuma na ƙarshe an fitar da su ta atomatik ta TGuide S16.
Sakamakon gwaji: jimillar naman dabbar RNA wanda TGuide S16 ya fitar ta atomatik sanye da reagent 4993551 yana da yawan amfanin ƙasa da tsabta, wanda yayi daidai da RNA da aka fitar ta hanyar ka'idar cire ginshiƙi. Tsaftace ainihin iri ɗaya ne, tare da OD260 / OD280 a kusa da 2.0 ~ 2.1 da OD260 / OD230> 2.0. A ƙarshe, ana iya amfani da TGuide S16 don cire jimillar RNA daga kyallen dabba don maye gurbin maganin cirewar tushen ginshiƙi.
