Eukararyotic m mma sequecting-ngs
Fasas
● Kulawa da Poly Mrna kafin shirye-shiryen laburare
● Zaɓin tsarin Darhari na Mista Comprary don kunna samun takamaiman bayanan da aka tsara
● Bioiniformatis na bincike na fili da tsarin rubutun
Yan fa'idohu
●Mai yawan ƙwarewa: Kungiyarmu ta sarrafa sama da samfuran 600,000 a Bmkgene, nau'ikan samfurori daban-daban irin su tantanin halitta, kyallen takarda, da ruwa. Mun kawo kwarewa ga kowane aiki kuma mun samu nasarar kammala ayyukan Mrna-SeQ sama da 100,000 a cikin yankin bincike daban-daban.
●Tsauri mai inganci: Muna aiwatar da wuraren sarrafawa a duk faɗin duk matakai, daga samfurin da ɗakin karatu zuwa sequincing da kuma yin amfani da biinhormatics. Wannan sauke mai sa ido yana tabbatar da isar da ingantaccen sakamako, tabbatar muku da cewa aikinku yana cikin amintaccen hannun.
●Cikakken bayani: Muna amfani da bayanai da yawa don aiwatar da shahararrun halittu daban-daban (DOGs) kuma suna yin amfani da nazarin haɓaka. Wannan cikakkiyar hanyar tana samar da fahimta cikin hanyoyin kwayoyin halitta da ke haifar da amsar da aka ba da amsa, tabbatar da cewa an sanar da ku game da sakamakon aikinku.
●Tallafin Bayanan Wasanni: Jagorarmu ta shimfida bayan kammala aikin tare da lokacin 3 bayan sayar da sabis. A wannan lokacin, muna bayar da fa'idar bi, don taimako na matsala, da kuma zaman don magance duk wasu tambayoyin da suka shafi sakamakon.
Bukatar samfuri da isarwa
| Leburare | Tsarin Sequining | Bayanai da shawarar | Iko mai inganci |
| Poly wani yanki | Illumina Pe150 DNBSEQ-T7 | 6 -10 GB | Q30≥85% |
Bukatun Samfura:
Nucheotides:
| Conc. (NG / μL) | Adadin (μg) | M | Kirki | |
| Standary ɗakin karatu | ≥ 10 | ≥ 0.2 | OD260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Iyakantacce ko babu furotin ko gurbataccen DNA wanda aka nuna akan gel. | Don tsire-tsire: Rinaw4.0; Ga dabbobi: Rinaw4.5; 5.0≥28s / 18S≥1.0; iyakantacce ko babu wani yanki |
| Liburrary Digiri | ≥ 10 | ≥ 0.2 | OD260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Iyakantacce ko babu furotin ko gurbataccen DNA wanda aka nuna akan gel. | Don tsire-tsire: Rinaw4.0; Ga dabbobi: Rinaw4.5; 5.0≥28s / 18S≥1.0; iyakantacce ko babu wani yanki |
● tsire-tsire:
Tushen, kara ko petal: 450 mg
Ganye ko iri: 300 mg
'Ya'yan itace: 1.2 g
●Dabba:
Zuciya ko hanji: 300 mg
Viscera ko kwakwalwa: 240 mg
Tsoka: 450 mg
Kasusuwa, gashi ko fata: 1g
● arthropods:
Kwari: 6g
Crustacea: 300 mg
Laifi gaba daya: 1 bututu
Kwayoyin sel: 106 sel
Bayar da Shawara
Akwati: 2 ml centrifuge bututu (tin capil ba da shawarar)
Allalan Samfura: Group + ya dace da misali a1, A2, A3; B1, B2, B3 ... ...
Jirgin ruwa:
1. Bushe-kankara: samfures bukatar a cushe a cikin jaka da binne a bushe-kankara.
2. Tables rnas tebur za a iya bushe a cikin RNA ,®) kuma an tura su cikin dakin da zazzabi.
Aikin aikin sabis
Tsarin gwaji
Samfura
Hakar RNA
Kungiyar Kwadadi
Kazalala
Bincike na bayanai
Bayan Siyarwa
Bioinformatics
Eukaryototic Binciken Mrna Sequefing Aiki
Bioinformatics
ØRaw bayanai
ØMagana da jeri na gida
ØBinciken Masana'o
ØUptiction shaida
ØBambancin bayani na daban
ØAyyukan ANGIP da wadatarwa
Magana da jeri na gida
Jikina
Samfurin daidaituwa da kimantawa game da abubuwan kwaikwayo na halittu
Daban-daban ya bayyana kwayoyin halitta (dogs)
Bayanin magana na Dogs
Yin amfani da Dogs
Bincika cigaban sauƙaƙe ta hanyar Bmkgene na Eukaryics na Misquin Search a cikin ayyukan Curated tarin tarin wallafe-wallafe.
Huang, L. et al. (2023) 'TRICLOSAN DA TRICLOCARCARAN YAKE ILLFACKEDRAKIYYA NA OLFACK IN CIGABA DA IYALI, DA TAFIYA TAFIYA AIKIN SAUKI', Binciken ruwa, 233, p. 119736. Doi: 10.1016 / j.watres.2023.119736.
Jia, LJ et al. (2023) 'Amma Aspergillus Fumijstas Hijacks p11 don sake fasalin fungal-dauke da fungasomes fungal-dauke da zuwa hanyar da ba ta lalata ba', rundunar kwayar halitta & microbe, 31 (3), PP. 373-388.e10. Doi: 10.1016 / j.chom.020.02.002.
Jin, K. et al. (2022) 'Abubuwan da aka lissafa TCP da suka shafi ci gaban MA BOAMBO (Dendrocalus Ratilorus Munro)', iyakoki a cikin ilimin shuka, 13, p. 884433. Doi: 10.3389 / FPLS.2022.84443 / BOTtex.
Wen, X. et al. (2022) 'Gwardar Lavanduliif ta Lavandulififum Lavandulififum da Tsarin kwayar halittar Capitululum iri', Binciken Ma'aikata, 9.1093 / HR / UHab022.
Zhang, Yujie et al. (2023) 'ANANORACAC don inganta maganin sonder a kan abin da ke cikin mahaifa ta hanyar karagewa ros gros tsaka-tsaki', Nano a yau, 49, p. 101798. Doi: 10.1016 / j.nantod.22023.10179.
