●Dandamali:Novumina Novaseq 6000 da Novaseq X Plus Plus
●Yanayin Sequinate:Pe150 da Pe250
●Ingancin ingancin ɗakunan karatu kafin sequencing
●Bayanin Rubuta Qc da Isar da kai:Isar da Rahoton QC da Raw bayanai a cikin tsarin Fastq bayan Demultiplexing da tace Q30 Karatu
●Orarancin ayyukan sequin:Abokin ciniki na iya zaɓar jerin abubuwa ta hanyar layi, mai kwarara cell, ko ta adadin bayanan da ake buƙata (ɓangare ɓangare naúrar).
●Kwarewa mai yawa akan dandamali na yau da kullun:tare da dubban rufaffiyar ayyukan tare da nau'ikan daban-daban.
●Isar da Rahoton Qc:Tare da ingancin awo, daidaitaccen bayanai kuma gaba ɗaya aikin aikin aikin.
●Tsarin Sequinate girma:tare da gajeren lokaci.
●Tsauri mai inganci: Muna aiwatar da bukatun QCT don bayar da garantin isar da sakamako mai inganci.
Tukuba | Cell mai gudana | Yanayin Sequining | Guda ɗaya | An kiyasta fitarwa |
Novasq x | 10b (8 lana) | Pe150 | Guda lane Ɓangaren ɓangaren lane | 375GB / Lane |
25B (8 hanyoyi) | Pe150 | Guda lane Ɓangaren ɓangaren lane | 1000 GB / Lane | |
NOVaseQ 6000 | Spelet Spell (2 hanyoyin 2) | Pe250 | Cell mai gudana Guda lane Ɓangaren ɓangaren lane | 325-400 m Karanta / Lane |
S4 SEL (4 LAYA) | Pe150 | Cell mai gudana Guda lane Ɓangaren ɓangaren lane | ~ 800 gb / layi |
Adadin bayanai (x) | Taro (QPCR / NM) | Girma | |
M layin sequincing
| X ≤ 10 gb | 1 nm | ≥ 25 μl |
10 GB <x ≤ 50 gb | 2 nm | ≥ 25 μl | |
50 GB <x ≤ 100 gb | ≥ 3 nm | ≥ 25 μl | |
X> 100 GB | ≥ 4 nm | ≥ 25 μl | |
Lane Sequincing | Kowane layi | ≥ 1.5 nm / dakin wanka | Yawan 25 μL / dakin wanka |
Baya ga maida hankali da adadin adadin, ana buƙatar tsarin ƙwanƙwasa.
SAURARA: LANAR-LAYIN LADIRA NA LABARIN LOBRANALILILILILILILILILILILILILILILILILILILILILALI NA FARKO NA FARKO NA FARKO - don tabbatar da kira mai robust.
Muna ba da shawarar ƙaddamar da ɗakunan ɗakunan karatu a matsayin samfurori. Idan kuna buƙatar Bmkgene don yin tafkin ɗakin karatu, don Allah a koma zuwa
bukatun laburaren don ɓangaren ɓangaren ɓangare na ɓangare.
Babban ganiya ya kamata ya kasance cikin 300-450 BP.
Ya kamata ɗakunan ɗakunan karatu guda ɗaya suna da babban kololuwa guda, babu gurbataccen rashin daidaitawa kuma ba tsintsaye na shareta ba.
Rahoton akan ingancin ɗakin karatu wanda aka tanada kafin sequincing, kimanta adadin laburare, da kuma rarrabuwa.
Tebur 1. Kididdiga akan bayanan Sequincation.
Sample ID | Bmkid | Raw ya karanta | Raw bayanai (BP) | Tsabtace karanta (%) | Q20 (%) | Q30 (%) | GC (%) |
C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Hoto na 1. Rarraba inganci tare a cikin kowane samfurin
Hoto na 2. Rarrabawa Abubuwan Masaba
Hoto na 3. Rarraba bayanan abin da ke ciki a cikin bayanan Sequincing bayanai