Cikakken manna sequincing -pacbio
Fasas
● CdNA SYNTHESS DAGA POLY-Mrna ta bi ta hanyar shirye-shiryen laburare
● Maƙƙa a yanayin CCS, samar da Hifi ya karanta
● Rubuta na cikakken bayanan sakamako
● Bincike ba ya wajabta da halittar ƙarshe. Koyaya, ana iya aiki
Binciken bincike na Gudanarwa yana ba da bincike game da bincike game da fassarar Ifofrna, Gene fushin Gene, poly-adenylation, da tsarin kwayoyin
Fa'idodi na sabis
●Babban daidaito: Hifi ya karanta tare da daidaito> 99.9% (Q30), Matsala da Ngs
● Sabuwar bincike na bincike: Sequinationungiyar duka fassarar tana ba da izinin ganowa da halayensu
●Mai yawan ƙwarewa: Tare da rikodin waƙar ya kammala sama da ayyukan PACbio sama da 1100 da kuma aiki sama da samfurori 2300, ƙungiyarmu tana kawo ƙwarewa ga kowane aiki.
●Tallafin Bayanan Wasanni: Jagorarmu ta shimfida bayan kammala aikin tare da lokacin 3 bayan sayar da sabis. A wannan lokacin, muna bayar da fa'idar bi, don taimako na matsala, da kuma zaman don magance duk wasu tambayoyin da suka shafi sakamakon.
Bukatar samfuri da isarwa
| Leburare | Tsarin Sequining | Bayanai da shawarar | Iko mai inganci |
| Polya ta wadatar da Laburaren Mna CCS | Pacbio Sequel II Pacbio revio | 20/40 GB 5/10 m CCS | Q30≥85% |
Bukatun Samfura:
Nucheotides:
● tsire-tsire:
Tushen, kara ko petal: 450 mg
Ganye ko iri: 300 mg
'Ya'yan itace: 1.2 g
● dabba:
Zuciya ko hanji: 300 mg
Viscera ko kwakwalwa: 240 mg
Tsoka: 450 mg
Kasusuwa, gashi ko fata: 1g
● arthropods:
Kwari: 6g
Crustacea: 300 mg
Laifi gaba daya: 1 bututu
Kwayoyin sel: 106 sel
| Conc. (NG / μL) | Adadin (μg) | M | Kirki |
| 100 | 1.0 | OD260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Iyakantacce ko babu furotin ko gurbataccen DNA wanda aka nuna akan gel. | Don tsire-tsire: Rintox7.5; Ga dabbobi: Rinet8.0; 5.0≥ 28S≥1.0; iyakantacce ko babu wani yanki |
Bayar da Shawara
Akwati: 2 ml centrifuge bututu (tin capil ba da shawarar)
Allalan Samfura: Group + ya dace da misali a1, A2, A3; B1, B2, B3.
Jirgin ruwa:
1. Bushe-kankara: samfures bukatar a cushe a cikin jaka da binne a bushe-kankara.
2. Tumbunan rniastable: Ana iya bushewa sasƙarin RNA a cikin bututun RNA, og rnastable®) kuma an shigo cikin zazzabi a ɗakin.
Aikin aikin sabis
Tsarin gwaji
Samfura
Hakar RNA
Kungiyar Kwadadi
Kazalala
Bincike na bayanai
Bayan Siyarwa
Ya hada da bincike mai zuwa:
Ikon ingancin bayanai
● Zaɓin Binciken Polyadenyisati mai zurfi (Apa)
● Labulen fassarar bayanan bincike
● Sabuwar bincike na bincike
● perchmuring ta duniya-kwafin orthologs (Busco) na bincike
● Littattafan Rubuce-rubucen Bincike: Hasashen Tsararren jerin lambobi (CDs) da Fasali mai aiki
Tarihin ● LNCRNA: Hasashen LCRNNA da kuma hari
Shaida Micressate tantancewa (SSR)
Bincike na Bincike
Madadin bincike na bincike
Mafarkin Polyadenymani na Polyadenyis (Apa)
Bayanin bayanin labari mai aiki
Bincika ci gaba da ci gaba sauƙaƙe a cikin wannan sabis na Mrkgene na Mrkgene a cikin wannan littafin fasalin.
Ma, Y. et al. (2023) na bincike na Pacbio da Ont RNAT RNA na biyu don nemopilema Nomurai Venom., Genomics, 115 (6), PA. 110709. Doi: 10.1016 / j.ygeno.2023.10709.
Chao, Q. et al. (2019) '' Yancin Tasirin Populus Taro TransCriptome ', dasa maganin halittar kere, 17 (1), PP. 206-219. Doi: 10.1111 / pbi.12558.
Deng, H. et al. (2022) 'Canje-canje a cikin abun ciki na ASCORBIC A cikin ci gaban' ya'yan itace (ruwan 'ya'yan itace-ascorbular-wadataccen kwayoyin, na ƙasa da kwayoyin halitta, da ƙasa ta kwayoyin halitta, (10), p. 5808. Doi: 10.3390 / ijms23105808 / s1.
Hua, X. et al. (2022) 'Hasashen tsinkayar hanyoyin kwayoyin halittu na biosylyplullins a cikin paris parlyllinlins a Paris 20: 1, 5 (1), PP. 1-10. Doi: 10.1038 / s42003-022-03000-z.
Liu, M. et al. (2023) 'Haɗe Pacbio Iso-Seq da Illumis RNA-SeQ da kuma rashin bincike game da Tuta. 363. Doi: 10.3390 / kwari14040363 / s1.
Wang, lijun et al. (2019) 'Binciken bincike na rashin daidaituwa ta amfani da Pacbio mai sharhi a hade da ingantacciyar fahimtar Ricinoleic, 20 (1), PP. 1-17. Doi: 10.1186 / s12864-019-5832-9.
