Binciken bincike na tantancewa
Fa'idodi na sabis
Takagi et al., Jaridar shuka, 2013
● CIGABA: Haɗa yawaita Bulks tare da 30 + 30 zuwa 200 zuwa 200 200 + mutum 200 don rage amo na baya; wanda ba a saba da shi ba na ɗan ƙaramin yanki na yanki na yanki.
Iltirƙiri na bincike: Cikakken ɗan ƙaramin aikin garu na aiki, ciki har da NR, Swiss, Kogs, Kogs, da sauransu.
Lokaci mai sauri: Lokaci mai sauri: Rashin Tsarin Gashin a cikin kwanaki 45 na aiki.
Mallagara da yawa: BMK ya ba da gudummawa a cikin dubban halaye, suna rufe nau'ikan nau'ikan halaye, samfuran samfuran ruwa, fure, fure, 'ya'yan itace, da sauransu.
Bayani na Sabis
Yawan jama'a:
Rarrabuwar kawuna na iyaye da ke adawa da fasinjoji.
Misali na F2, Pencrossing (BC), an gabatar da layin da aka shigar (RIL)
Haɗuwa ga tafiye-tafiye
Don halaye masu cancanta: 30 zuwa 50 mutane (mafi ƙarancin 20) / BUlK
Don strattisative Tratis: Top 5% zuwa 10% mutane tare da ko dai matsanancin ɓarna a cikin duka yawan jama'a (mafi karancin 30 + 30).
Zuburinku na sequencation
Aƙalla 20x / mahaifa da 1x / zuriyar mutum
Binciken Bioinformatics nazarin
● Duk masu kama da gaske
● Gudanar da Bayani
● snp / indel kira
Shawarar dan takarar yankin
● Mai karbi Maimuri Genear
Bukatar samfuri da isarwa
Bukatun Samfura:
Nucheotides:
| GDNA Samfura | Samfuran nama |
| Taro: ≥30 ng / μl | Tsire-tsire: 1-2 g |
| Adadin: ≥2 μg (Ch vumn ≥15 μl) | Dabbobi: 0.5-1 g |
| Tsarkake: OD260 / 280 = 1.6-2.5 | Jin jini duka: 1.5 ml |
Aikin aikin sabis
Tsarin gwaji
Samfura
Hakar RNA
Kungiyar Kwadadi
Kazalala
Bincike na bayanai
Bayan Siyarwa
1.Shasis na bincike na lokaci akan Euclidean nesa (ED) don gano yankin ɗan takarar. A adadi mai zuwa
X-Axis: Lambar Chromosome; Kowane dot yana wakiltar darajar snp. Layi na baki ya dace da darajar Ed. Darajar Ed ta fi girma tana nuna wata ma'amala ta musamman tsakanin shafin da kuma phenotype. Red dash yana wakiltar ƙofar mahimmin tarayya.
2.Alassis na rubutu ba tare da alamar Snp-ba
X-Axis: Lambar Chromosome; Kowane dot yana wakiltar darajar SNP-Index. Layin baki yana tsaye don dacewa da darajar Snp-Index. Babban darajar shine, mafi muhimmanci kungiyar take.
BMK lamari
Babban sakamako mai inganci Locus FNL7.1 Eccodes a ƙarshen marigayi fruerener hade da tsayin dako a cikin kokwamba
Aka buga: Shuka Jaridar Kanna, 2020
Tsarin Sequinate dabarar:
Iyaye (Jin5-508, yn): Dukkanin suttura na ainihi don 34 × da 20 ×.
DNA POOLS (50 da dogon lokaci-daɗaɗɗa ne): sake sarrafawa don 61 × da 52 × da 52 × da 52 × da 52 × da 52 × da 52 × da 52 × da 52 × da 52 × da 52 ×
Sakamakon sakamako
A cikin wannan binciken, rarraba yawan jama'a (F2 da F2: 3) an samar da layin dogon kokwamba mai wuyan wuyan wuy-wuy ruwa. Mutane biyu na tafiye-tafiye sun gina ta 50 matsanancin wuya mutane da kuma mutane 50 masu rauni kaɗan. An gano manyan tasirin QTL akan Chr07 ta nazarin BSA da taswirar Qtl na gargajiya. Yankin yankin ya kara murkushe shi da dan takarar mai inganci, jumla mai faɗi da gwaje-gwaje na Transgenic, wanda aka saukarwar makult gwaje-gwaje, wanda aka bayyana makullin gene wajen sarrafa wuyan wuyansa, CSFNL7.1. Bugu da kari, an gano yankin Polymorph a cikin CSFNL7.1 Ana samun yankin mai gabatarwa na Polyl7.1. Matun da Phylogenetic ya ba da shawarar cewa FNL7.1 Locu alama da alama za a samo asali daga Indiya.
 QTl-Mappping a cikin binciken BSA don gano yankin ɗan takarar da ke hade da tsawon wuya |  Bayanan bayanan bayanan Lod na kokwamba na kokwamba a kan Chr07 |
Xu, x., et al. "Babban sakamako mai tasiri na kayan aiki FnLux7.1 Eccodes a ƙarshen marigayi prryogener da ke hade da tsayin wuya a cikin kokwamba." Itace ilmin kimiya na kimiya na jarida 18.7 (2020).
