BMKMANU S1000 Spatial Transcriptome
Tsarin fasaha na BMKMANU S1000 Spatial Transcriptome
Siffofin
● Ƙaddamarwa: 5 µM
● Tabo Diamita: 2.5 µM
● Yawan tabo: kusan Miliyan 2
● Tsarukan yanki mai yuwuwa 3: 6.8 mm * 6.8 mm, 11 mm * 11 mm ko 15 mm * 20 mm
● Kowane ƙulli da aka ɗora ana ɗora shi da ginshiƙai wanda ya ƙunshi sassa 4:
wutsiya poly(dT) don ƙaddamar da mRNA da haɗin cDNA
Mai Gano Kwayoyin Halitta na Musamman (UMI) don gyara son zuciya na haɓakawa
Barcode na sarari
Jerin dauri na juzu'i na karanta juzu'i 1 na farko
● H&E da tabon sassa
● Yiwuwar amfanifasahar rabuwar salula: hadewar H&E tabo, mai kyalli, da kuma jerin RNA don ƙayyade iyakokin kowane tantanin halitta da kuma sanya bayanin kwayar halitta daidai ga kowane tantanin halitta.
Amfanin BMKMANU S1000
●Ƙaddamar Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙwara: Kowane yanki na kama yana ƙunshe da > Barcoded Spots miliyan 2 tare da diamita na 2.5 µm da tazara na 5 µm tsakanin wuraren tabo, yana ba da damar nazarin bayanan sararin samaniya tare da ƙudurin ƙaramin salula (5 μm).
●Ƙididdigar Ƙimar Maɗaukaki da yawa:Binciken matakai masu sassaucin ra'ayi daga 100 μm zuwa 5 μm don warware fasalin nama iri-iri a mafi kyawun ƙuduri.
● Yiwuwar Amfani da Fasahar Rabewar Tantanin halitta "Uku cikin zamewa ɗaya":Haɗa tabo mai kyalli, tabon H&E, da jerin RNA akan faifai ɗaya, binciken mu na "uku-cikin-ɗaya" algorithm yana ba da ikon gano iyakokin tantanin halitta don bayanan tushen tantanin halitta na gaba.
●Mai jituwa tare da Platforms Sequencing Multiple: Dukansu NGS da jerin dogon karantawa suna samuwa.
●Zane mai sassauƙa na yanki 1-8 Mai AikiGirman yanki mai sassauƙa, yana yiwuwa a yi amfani da tsari 3 (6.8mm * 6.8 mm., 11 mm * 11 mm and 15 mm * 20 mm)
●Sabis Tasha Daya: Yana haɗawa da duk ƙwarewa da matakai na tushen fasaha, ciki har da sashin cryo-sectioning, tabo, inganta kyallen takarda, ƙirar sararin samaniya, shirye-shiryen ɗakin karatu, sequencing, da bioinformatics.
●Cikakken Bioinformatics da Kallon Sakamako na Abokin Amfani:kunshin ya ƙunshi nazari 29 da ƙididdiga masu inganci 100+, haɗe tare da yin amfani da ingantaccen software na cikin gida don hangowa da tsara rarrabuwar tantanin halitta da tari.
●Nazarin Bayanai na Musamman da Kallon gani: akwai don buƙatun bincike daban-daban
●Ƙwararrun Ƙwararrun Ƙwararru: tare da gogewa a cikin nau'ikan nama sama da 250 da nau'ikan 100+ da suka haɗa da ɗan adam, linzamin kwamfuta, dabbobi masu shayarwa, kifi da tsirrai.
●Sabuntawa na ainihi akan Gabaɗayan Aikin: tare da cikakken iko na ci gaban gwaji.
●Nazari na Haɗin Haɗin Zaɓi tare da Sel na mRNA guda ɗaya
Ƙayyadaddun Sabis
|
Misali Abubuwan bukatu
| Laburare |
Dabarun jeri
| An ba da shawarar bayanai | Kula da inganci |
| Samfuran cryo da aka haɗa da OCT, tubalan 3 kowane samfurin | S1000 cDNA ɗakin karatu | Illumina PE150 (sauran dandamali akwai) | 100K PE ana karantawa akan 100 uM (60-150 Gb) | RIN> 7 |
Don ƙarin cikakkun bayanai kan jagorar shirye-shiryen samfuri da aikin sabis, da fatan za a ji daɗin magana da aMasanin BMKGENE
Gudun Aikin Sabis
A cikin lokacin shirye-shiryen samfurin, ana yin gwajin haƙar RNA na farko don tabbatar da samun RNA mai inganci. A cikin matakin inganta nama sassan sassan suna da tabo kuma an duba su kuma an inganta yanayin ƙetare don sakin mRNA daga nama. Sannan ana amfani da ingantacciyar ƙa'idar yayin aikin ginin ɗakin karatu, sannan a bi da bi da kuma nazarin bayanai.
Cikakken aikin sabis ɗin ya haɗa da sabuntawa na ainihi da kuma tabbatar da abokin ciniki don kula da madaidaicin ra'ayi, yana tabbatar da aiwatar da aikin mai santsi.
Ana nazarin bayanan da BMKMANU S1000 ya ƙirƙira ta hanyar amfani da software "BSTMatrix", wanda BMKGENE ya tsara ta kansa, yana samar da Matrix na Maganar Gene. Daga can, ana samar da daidaitaccen rahoto wanda ya haɗa da sarrafa ingancin bayanai, bincike-bincike na ciki da bincike tsakanin ƙungiyoyi.
● Kula da ingancin bayanai:
Fitowar bayanai da rarraba makin inganci
Gano kwayoyin halitta a kowane tabo
Rufin nama
● Binciken samfurin ciki:
Gene wadata
Tarin tabo, gami da raguwar ƙididdigar girma
Bambance-bambancen maganganun magana tsakanin gungu: gano kwayoyin halitta masu alama
Bayanin aiki da haɓaka ƙwayoyin halitta masu alama
● Binciken Tsakanin ƙungiyoyi:
Sake haɗa tabo daga samfuran biyu (misali marasa lafiya da sarrafawa) da sake tari
Gano kwayoyin halitta masu alamar ga kowane tari
Bayanin aiki da haɓaka ƙwayoyin halitta masu alama
Bambance-bambancen gungu iri ɗaya tsakanin ƙungiyoyi
Bugu da ƙari, BMKGENE ya haɓaka "BSTViewer" kayan aiki ne na abokantaka wanda ke ba mai amfani damar hango maganganun kwayoyin halitta da tabo tari a matakai daban-daban.
BMKGene ya haɓaka software don hangen nesa na abokantaka
Tarin tabo BSTViewer a ƙudurin matakai da yawa
BSTCellViewer: Rarraba tantanin halitta ta atomatik da hannu
Nazari-samfurin ciki
Tarin tabo:
Gane kwayoyin halitta masu alamar alama da rarraba sarari:
Binciken Inter-group
Haɗin bayanai daga rukunoni biyu da sake tari:
Alamar kwayoyin halittar sabbin tari:
Bincika ci gaban da BMKGene's spatial transcriptomics services tare da fasahar BMKManu S1000 ke gudanarwa a cikin wannan fitaccen ɗaba'ar:
Song, X. et al. (2023) 'Spatial transcriptomics yana bayyana ƙwayoyin chlorenchyma da ke haifar da haske waɗanda ke da hannu wajen haɓaka farfadowar harbi a cikin callus tumatir',Abubuwan da aka ɗauka na Cibiyar Nazarin Kimiyya ta Ƙasa ta Amurka ta Amurka, 120 (38), shafi. e2310163120. doi: 10.1073/pnas.2310163120
Ku, Y. et al. (2023) 'Kwantawar tsari na hanyoyin da suka dogara da bayanan sararin samaniya',bioRxiv, p. 2023.12.03.569744. doi: 10.1101/2023.12.03.569744.
